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http://dx.doi.org/10.5369/JSST.2011.20.6.400

Development of Disposable Enzyme-linked Immunosensor Strip Platform  

Choi, Ji-Hye (Institute of BioPhysio Sensor Technology, Pusan National University)
Yi, Seung-Jae (School of Mechanical Engineering, Pusan National University)
Chang, Seung-Cheol (Institute of BioPhysio Sensor Technology, Pusan National University)
Kim, Kyung-Chun (School of Mechanical Engineering, Pusan National University)
Publication Information
Journal of Sensor Science and Technology / v.20, no.6, 2011 , pp. 400-405 More about this Journal
Abstract
This study introduced the development of a strip type disposable enzyme-linked immunosensor platform for the detection of IgG. Strips of the strip sensor were fabricated by using commercial nitrocellulose filter membranes and a housing holder for the strips was manufactured by using a standard injection molding process for a plastic material. An IgG-urease conjugate was prepared and used for the competitive immune-binding with sample IgG. From the enzymatic reaction between the conjugated urease and urea added, ammonia was generated and caused a localized alkaline pH change on the immobilized antibody band which was coated onto the sensor strips. This pH increase subsequently caused a color change of the antibody band in the presence of a pH indicator, phenol red. Used in conjunction with a competitive immunoassay format, the intensity of the color produced is directly linked with the concentration of target analyte, IgG, and specific measurement of IgG in a lateral flow immunoassay format was achieved over the range 100 ppb to 2000 ppb IgG.
Keywords
Enzyme-Linked Immunosensor; Disposable; Strip Sensor; IgG;
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Times Cited By KSCI : 5  (Citation Analysis)
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