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http://dx.doi.org/10.5369/JSST.2011.20.4.226

Characteristics of Protein G-modified BioFET  

Sohn, Young-Soo (Department of Biomedical Engineering, Catholic Unversity of Daegu)
Publication Information
Journal of Sensor Science and Technology / v.20, no.4, 2011 , pp. 226-229 More about this Journal
Abstract
Label-free detection of biomolecular interactions was performed using BioFET(Biologically sensitive Field-Effect Transistor) and SPR(Surface Plasmon Resonance). Qualitative information on the immobilization of an anti-IgG and antibody-antigen interaction was gained using the SPR analysis system. The BioFET was used to explore the pI value of the protein and to monitor biomolecular interactions which caused an effective charge change at the gate surface resulting in a drain current change. The results show that the BioFET can be a useful monitoring tool for biomolecular interactions and is complimentary to the SPR system.
Keywords
BioFET; Protein G; SPR;
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