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http://dx.doi.org/10.5369/JSST.2008.17.5.380

Immunoassay of haptoglobin and transferrin with proteinG-containing QCM sensor chip and unpurified antiserum  

Ha, In-Young (Department of Chemistry, Kangnung National University)
Choi, Suk-Jung (Department of Chemistry, Kangnung National University)
Publication Information
Journal of Sensor Science and Technology / v.17, no.5, 2008 , pp. 380-386 More about this Journal
Abstract
Quartz crystal microbalance immunosensor has a capacity to perform a label-free and real time detection of a trace amount of analyte through the specific interaction between antibody and antigen. However, immobilization of antibody molecules on the sensor surface is a troublesome procedure for researchers who are not experienced in chemistry. Protein G has a specific affinity to antibody and would serve as a capturing agent for antibody when immobilized on the sensor surface. In this work, we prepared a protein G sensor chip by immobilizing protein G on the surface of quartz crystal microbalance and examined its capability to detect human haptoglobin or human transferrin with unpurified corresponding antiserum. Specific and dose dependent response was observed when the protein G chip was used for detection of antigens after saturated with antiserum. We also verified several advantageous aspects of the protein G chip such as improved flexibility and sensitivity.
Keywords
protein G; immunosensor; quartz crystal microbalance(QCM); haptoglobin; transferrin;
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