Browse > Article
http://dx.doi.org/10.5369/JSST.2008.17.1.029

Tri-enzyme modified electrochemical biosensor for paracetamol detection  

Park, Deog-Su (Center for Innovative Bio. Physio Sensor Technology, Pusan National University)
Shim, Yoon-Bo (Department of Chemistry, Pusan National University)
Chang, Seung-Cheol (Center for Innovative Bio. Physio Sensor Technology, Pusan National University)
Publication Information
Journal of Sensor Science and Technology / v.17, no.1, 2008 , pp. 29-34 More about this Journal
Abstract
A new disposable amperometric tri-enzyme biosensor for the detection of paracetamol has been developed. The paracetamol sensors developed uses horseradish peroxidase modified screen-printed carbon electrodes (HRP-SPCEs) coupled with immobilized enzymes, tyrosinase and aryl acylamidase, prepared using a poly (vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ) matrix. Optimization of the experimental parameters has been performed and the paracetamol biosensor showed detection limit for paracetamol is as low as $100{\mu}M$ and the sensitivity of the sensor is $1.46nA{\mu}M^{-1}cm^{-2}$.
Keywords
paracetamol; screen printing; amperometric and enzyme electrode;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 S. C. Chang, K. Rawson, and C. J. McNeil, 'Disposable tyrosinase-peroxidase bi-enzyme sensor for amperometric detection of phenols', Biosensors & Bioelectronics, vol. 17, pp. 1015-1023, 2002   DOI   ScienceOn
2 O. W. Lau, S. F. Luk, and Y. M. Cheung, 'Simultaneous determination of ascorbic acid, caffeine and paracetamol in drug formulations by differential- pulse voltammetry using a glassy carbon electrode', Analyst, vol. 114, pp. 1047-1051, 1989   DOI
3 M. L. Ramos, J. F. Tyson, and D. J. Curran, 'Determination of acetaminophen by flow injection with on-line chemical derivatization: Investigations using visible and FTIR spectrophotometry', Analytica Chimica Acta, vol. 364, pp. 107-116, 1998   DOI   ScienceOn
4 F. W. Scheller, U. Wollenberger, A. Warsinke, and F. Lisdat, 'Research and development in biosensors', Current Opinion in Biotechnology, vol. 12, pp. 35-40, 2001   DOI   ScienceOn
5 S. C. Chang and D. S. Park, 'Disposable in-field electrochemical potable sensor system for free available chlorine (FAC) Detection', J. Korean Sensors Society, vol. 16, no. 6, pp. 449-456, 2007   과학기술학회마을   DOI
6 G. A. Messina, I. E. De Vito, and J. Raba, 'On-line microfluidic sensor integrated with an enzymemodified pre-cell for the monitoring of paracetamol in pharmaceutical samples', Analytica Chimica Acta, vol. 559, pp. 152-158, 2006   DOI   ScienceOn
7 S. F. Wang, F. Xie, and R. F. Hu, 'Carbon-coated nickel magnetic nanoparticles modified electrodes as a sensor for determination of acetaminophen', Sensors and Actuators B-Chemical, vol. 123, pp. 495-500, 2007   DOI   ScienceOn
8 A. Criado, S. Cardenas, M. Gallego, and M. Valcarcel, 'Continuous flow spectrophotometric determination of paracetamol in pharmaceuticals following continuous microwave assisted alkaline hydrolysis', Talanta, vol. 53, pp. 417-423, 2000   DOI   ScienceOn
9 M. K. Srivastava, S. Ahmad, D. Singh, and I. C. Shukla, 'Titrimetric determination of dipyrone and paracetamol with potassium hexacyanoferrate(III) in an acidic medium', Analyst, vol. 110, pp. 735-737, 1985   DOI
10 J. L. Vilchez, R. Blanc, R. Avidad, and A. Navalon, 'Spectrofluorimetric determination of paracetamol in pharmaceuticals and biological fluids', J. Pharmaceutical and Biomedical Analysis, vol. 13, pp. 1119-1125, 1995   DOI   ScienceOn
11 K. K. Verma, A. Jain, and K. K. Stewart, 'Flowinjection spectroptometric determination of acetaminophen in drug formulations', Analytica Chimica Acta, vol. 261, pp. 261-267, 1992   DOI   ScienceOn
12 G., F. Cespedes, S. Alegret, and M. del Valle, 'Sequential injection system with higher dimensional electrochemical sensor signals Part 1. Voltammetric e-tongue for the determination of oxidizable compounds', Talanta, vol. 66, pp. 1187-1196, 2005   DOI   ScienceOn
13 J. Roy, P. Saha, S. Sultana, and A.S. Kenyon, 'Rapid screening of marketed paracetamol tablets: Use of thin-layer chromatography and a semiquantitative spot test', Bulletin of the World Health Organization, vol. 75, pp. 19-22, 1997
14 S. Ravisankar, M. Vasudevan, M. Gandhimathi, and B. Suresh, 'Reversed-phase HPLC method for the estimation of acetaminophen, ibuprofen and chlorzoxazone in formulations', Talanta, vol. 46, pp. 1577-1581, 1998   DOI   ScienceOn
15 Y. T. Lee and S. R. Lee, 'Development of the disposable glucose sensor using Cu/Ni/Au electrode', J. Korean Sensors Society, vol. 15, no. 5, pp. 352-356, 2006   과학기술학회마을   DOI
16 P. Routledge, J. A. Vale, D. N. Bateman, G. D. Johnston, A. Judd, S. Thomas, G. Volans, L. F. Prescott, and A. Proudfoot, 'Paracetamol (acetaminophen) poisoning-No need to change current guidelines to accident departments', British Medical Journal, vol. 317, pp. 1609-1610, 1998   DOI   ScienceOn
17 A. Vale, 'Paracetamol (acetaminophen)', Medicine, vol. 35, pp. 643-645, 2007   DOI   ScienceOn
18 P. J. Zed and E. P. Krenzelok, 'Treatment of acetaminophen overdose', American J. of Health System Pharmacy, vol. 56, pp. 1081-1091, 1999
19 L. de Haro, L. Tichadou, N. Prost, C. Perringue, G. Drouet, F. Rodor, M. Valli, and J. Arditti, 'Accidental ingestion of paracetamol in the form of paediatric syrup (EFFERALGAN). Experience of marseille poison control centre in 1998', Therapie, vol. 54, pp. 771-773, 1999
20 E. Dinc, C. Yucesoy, and F. Onur, 'Simultaneous spectrophotometric determination of mefenamic acid and paracetamol in a pharmaceutical preparation using ratio spectra derivative spectrophotometry and chemometric methods', J. Pharmaceutical and Biomedical Analysis, vol. 28, pp. 1091-1100, 2002   DOI   ScienceOn