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http://dx.doi.org/10.5369/JSST.2006.15.3.158

Microfluidic immunoassay using superparamagnetic nanoparticles in an enhanced magnetic field gradient  

Hahn, Young-Ki (Department of BioSystems, KAIST)
Kang, Joo-H. (Department of BioSystems, KAIST)
Kim, Kyu-Sung (Department of BioSystems, KAIST)
Park, Je-Kyun (Department of BioSystems, KAIST)
Publication Information
Journal of Sensor Science and Technology / v.15, no.3, 2006 , pp. 158-163 More about this Journal
Abstract
This paper reports a novel immunoassay method using superparamagnetic nanoparticles and an enhanced magnetic field gradient for the detection of protein in a microfluidic device. We use superparamagnetic nanoparticles as a label and fluorescent polystyrene beads as a solid support. Based on this platform, magnetic force-based microfluidic immunoassay is successfully applied to analyze the concentration of IgG as model analytes. In addition, we present ferromagnetic microstructure connected with a permanent magnet to increase magnetic flux density gradient (dB/dx, ${\sim}10^{4}$ T/m), which makes limit of detection reduced. The detection limit is reduced to about 1 pg/mL.
Keywords
microfluidics; immunoassay; magnetic nanoparticles;
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