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http://dx.doi.org/10.14776/piv.2016.23.3.172

Molecular Epidemiology of Bacillus cereus in a Pediatric Cancer Center  

Kim, Jong Min (Department of Pediatrics, Myongji Hospital, Seonam University College of Medicine)
Park, Ki-Sup (Center for Clinical Research, Samsung Biomedical Research Institute)
Lee, Byung-Kee (Department of Pediatrics, Good Gang-An Hospital)
Kim, Soo Jin (Center for Clinical Research, Samsung Biomedical Research Institute)
Kang, Ji-Man (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Kim, Yanghyun (Center for Clinical Research, Samsung Biomedical Research Institute)
Yoo, Keon Hee (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Sung, Ki Woong (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Koo, Hong Hoe (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Lee, Nam Yong (Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Kim, Yae-Jean (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Publication Information
Pediatric Infection and Vaccine / v.23, no.3, 2016 , pp. 172-179 More about this Journal
Abstract
Purpose: Bacillus cereus has been reported as the cause of nosocomial infections in cancer patients. In our pediatric cancer ward, a sudden rise in the number of patients with B. cereus bacteremia was observed in 2013 to 2014. This study was performed to investigate the molecular epidemiology of increased B. cereus bacteremia cases in our center. Methods: Pediatric cancer patients who developed B. cereus bacteremia were identified from January 2001 to June 2014. The B. cereus bacteremia in this study was defined as a case in which at least one B. cereus identified in blood cultures, regardless of true bacteremia. Available isolates were further tested by multilocus sequence typing (MLST) analysis. A retrospective chart review was performed. Results: Nineteen patients developed B. cereus bacteremia during the study period. However, in 2013, a sudden increase in the number of patients with B. cereus bacteremia was observed. In addition, three patients developed B. cereus bacteremia within 1 week in July and the other three patients within 1 week in October, respectively, during emergency room renovation. However, MLST analysis revealed different sequence types without consistent patterns. Before 2013, five tested isolates were ST18, ST26, ST177, and ST147-like type, and ST219-like type. Isolates from 2013 were ST18, ST73, ST90, ST427, ST784, ST34-like type, and ST130-like type. Conclusions: MLST analyses showed variable ST distribution of B. cereus isolates. Based on this study, there was no significant evidence suggesting a true outbreak caused by a single ST among patients who developed B. cereus bacteremia.
Keywords
Bacillus cereus; Disease outbreaks; Multilocus sequence typing; Pediatrics; Neoplasms;
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