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Evaluation of Uricult Trio Test as a Rapid Screening of UTI in Children with Fever  

Lee, Jin (Department of Pediatrics, Han-il General Hospital)
Kim, Eun Jin (Department of Pediatrics, Han-il General Hospital)
Lee, Taek Jin (Department of Pediatrics, College of Medicine, Pochon CHA University)
Chang, Jin Keun (Department of Pediatrics, Han-il General Hospital)
Cha, Sung-Ho (Department of Pediatrics, College of Medicine, Kyunghee University)
Publication Information
Pediatric Infection and Vaccine / v.17, no.2, 2010 , pp. 74-82 More about this Journal
Abstract
Purpose : The Dipslide culture test is a rapid method for diagnosis of urinary tract infection (UTI). The aim of this study is to determine the diagnostic availability of a urine Dipslide test for evaluation of UTI in febrile children. Methods : Urine specimens from 151 febrile infants were inoculated by a routine blood agar urine culture method and the Dipslide test at the same time. Following incubation for 16-24 hours, the results of the Dipslide test were read at the next visit. Both results of Dipslide and those of routine culture were compared. Results : The mean age of subjects was 15${\pm}$10.6 months. There were 150 infants (99.3%) who had fever with a mean duration of 2.6${\pm}$2.6 days. Thirty two infants (21.2%) were diagnosed as having UTI. Sensitivity and specificity of Uricult Trio CLED medium were 59.4% and 84.8%, respectively. Sensitivity and specificity of Uricult Trio E. coli medium were 60.0% and 96.2%, respectively. The Pearson correlation coefficient between results of Uricult Trio CLED medium and urine culture was 0.438 (P=0.01). Correlation between results of Uricult Trio E. coli medium and urine culture was 0.617 (P=0.01). Conclusion : The Dipslide test requires only 16-24 hours with high specificity in terms of UTI caused by E. coli without the problems associated with specimen delay. This test seems to be helpful for exclusion of UTI in febrile infants and it may reduce unnecessary hospitalization and antibiotic use. However, further studies are required before the product can be recommended as a routine diagnostic tool.
Keywords
Urinary tract infection; Dipslide; Uricult Trio; Children;
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