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http://dx.doi.org/10.14702/JPEE.2022.327

Educational Peptide Mapping of Protein-based Biopharmaceuticals by using LC-MS/MS  

Kim, Junseok (Department of Biomedical Systems Engineering, Korea Polytechnics)
Publication Information
Journal of Practical Engineering Education / v.14, no.2, 2022 , pp. 327-332 More about this Journal
Abstract
This experiment presents a precise analysis method using a mass spectrometer in the biopharmaceutical market, where utility is expanding. Among various techniques for analyzing the protein drug, somatotropin, the peptide fragments through biochemical sample preparation was analyzed by LC-MS/MS characterization. The analysis process was performed by separation analysis using nanoUPLC and MS/MS analysis using Orbitrap. In the case of somatotropin with 21 tryptic peptides, 13 of them were consistent with theoretical predictions within an average of 1 ppm error.
Keywords
LC-MS/MS; Protein drug; Somatotropin; Tryptic peptide;
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