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http://dx.doi.org/10.13047/KJEE.2017.31.6.492

Genetic Diversity of Salicornia herbacea according to Habitat Area by ISSR Markers  

Kim, Suk-Kyu (Tidal Flat Research Center-National Institute of Fisheries Science)
Cho, Yoon Sik (Tidal Flat Research Center-National Institute of Fisheries Science)
Hur, Young Baek (Tidal Flat Research Center-National Institute of Fisheries Science)
Song, Jae Hee (Tidal Flat Research Center-National Institute of Fisheries Science)
Jeong, Hee Do (Tidal Flat Research Center-National Institute of Fisheries Science)
Chung, Sang Ok (Tidal Flat Research Center-National Institute of Fisheries Science)
Publication Information
Korean Journal of Environment and Ecology / v.31, no.6, 2017 , pp. 492-499 More about this Journal
Abstract
This study analyzed 96 individuals in 6 populations using ISSR marker to investigate the genetic diversity of Salicornia herbacea populations. The total of 49 PCR amplification bands was observed in 6 ISSR primers, and 30 of them had genetic polymorphisms. The Shannon's information index (I) and gene diversity index (h), which indicate the genetic diversity of the Salicornia herbacea populations, were 0.382 and 0.249, respectively. The genetic diversity according to the population size was lowest with 0.092 (I) and 0.058 (h) in $0.1m{\times}0.1m$ and highest with 0.338 (I) and 0.227 (h) in $25m{\times}25m$, which was suitable for the furtherance of the high population with high genetic diversity. The UPGMA dendrogram based on Nei's genetic distance did not show a significant correlation with the distance between the Salicornia herbacea population. The results indicate that the Salicornia herbacea habiting in the restricted environment should have an area over a certain size to ensure the formation of a population with genetic diversity.
Keywords
HALOPHYTE; POLYMORPHISMS; POPULATION SIZE; MINIMUM AREA;
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