Browse > Article
http://dx.doi.org/10.5668/JEHS.2021.47.5.479

Distribution and Characterization of Vibrio vulnificus Isolated in Coastal Areas of Chungcheongnam-do Province  

Lee, Hyunah (Chungcheongnam-do Institute of Health and Environment Research)
Ko, Young-Eun (Chungcheongnam-do Institute of Health and Environment Research)
Choi, Jihye (Chungcheongnam-do Institute of Health and Environment Research)
Lee, Dayeon (Chungcheongnam-do Institute of Health and Environment Research)
Yeo, Seoungsoon (Chungcheongnam-do Institute of Health and Environment Research)
Park, Jongjin (Chungcheongnam-do Institute of Health and Environment Research)
Lee, Miyoung (Chungcheongnam-do Institute of Health and Environment Research)
Choi, Jinha (Chungcheongnam-do Institute of Health and Environment Research)
Park, Junhyuk (Chungcheongnam-do Institute of Health and Environment Research)
Publication Information
Journal of Environmental Health Sciences / v.47, no.5, 2021 , pp. 479-485 More about this Journal
Abstract
Background: Vibrio vulnificus has been frequently detected in seawater, fish, and shellfish mainly in the coastal areas of Chungcheongnam-do Province. Objectives: This study was conducted to investigate the analyzed biochemical properties, genetic characteristics, and distribution of Vibrio vulnificus isolated from environmental sources in coastal areas of Chungcheongnam-do Province from 2019 to 2020. Methods: A total of 1,510 samples were obtained from six different sites in Chungcheongnam-do Province. Isolated strains from the samples were identified by a VITEK 2 system and MALDI-TOF. Antibiotic susceptibility testing for 85 isolates was done by microdilution minimum inhibitory concentration methods, and 11 isolates were analyzed for 16s rRNA sequences in multiple alignments. Results: Among the 1,510 samples taken during the investigation period, 306 strains were isolated and the detection rate of V. vulnificus was 20.3%. One hundred eighty-eight strains (24.6%) from seawater and 118 strains (15.8%) from mud flats were isolated. It was mainly detected in July (17.3%), August (36.5%), and September (28.8%), and the proportion was 82.0%. Based on the CLSI-recommended breakpoints, V. vulnificus isolates were all susceptible to amoxicillin/clavulanic acid, amikacin, gentamicin, ciprofloxacin and trimethoprim/sulfamethoxazole. However, nonsusceptible isolates to ampicillin, ampicillin/sulbactam, cefazolin, cefoxitin, imipenem, tetracycline and chloramphenicol were identified. In the analysis of the nucleotide sequences for 16s rRNA of V. vulnificus isolates, it was confirmed that mutations frequently occurred between nucleotide number 922 and 952, and 98.2% to 100% nucleotide identities between isolates was verified. Conclusions: The results of this study can be used as a basic data for infection control and prevention of Vibrio vulnificus infection by describing the distribution and characteristics of Vibrio vulnificus strains isolated in coastal areas of Chungcheongnam-do Province.
Keywords
16s rRNA sequence; antibiotic susceptibility; Chungcheongnam-do coastal areas; Vibrio vulnificus;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Shapiro RL, Altekruse S, Hutwagner L, Bishop R, Hammond R, Wilson S, et al. The role of Gulf Coast oysters harvested in warmer months in Vibrio vulnificus infections in the United States, 1988-1996. Vibrio Working Group. J Infect Dis. 1998; 178(3): 752-759.   DOI
2 Randa MA, Polz MF, Lim E. Effects of temperature and salinity on Vibrio vulnificus population dynamics as assessed by quantitative PCR. Appl Environ Microbiol. 2004; 70(9): 5469-5476.   DOI
3 Kim DM, Hong SJ. Vibrio vulnificus sepsis. Korean J Med. 2012; 82(6): 671-679.   DOI
4 Yang SJ, Shin JH, Cho D, Kee SJ, Shin MG, Suh SP, et al. Identification of Vibrio vulnificus by the Microscan and the Vitek II systems. Korean J Lab Med. 2005; 25(1): 33-38.
5 Elmahdi S, DaSilva LV, Parveen S. Antibiotic resistance of Vibrio parahaemolyticus and Vibrio vulnificus in various countries: a review. Food Microbiol. 2016; 57: 128-134.   DOI
6 Thompson FL, Gevers D, Thompson CC, Dawyndt P, Naser S, Hoste B, et al. Phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis. Appl Environ Microbiol. 2005; 71(9): 5107-5115.   DOI
7 Ministry of Food and Drug Safety. Prediction System of Vibrio Vulnificus. Available: http://vibrio.foodsafetykorea.go.kr/main [accessed 15 March 2021].
8 Hwang KW, Gong YW, Oh BY, Lee JM, Go JM, Kim YH. Distribution and characteristic of growth of Vibrio spp. in Incheon coastal area. J Environ Sanit Eng. 2008; 23(3): 31-38.
9 Jeong YI, Myung GE, Choi EJ, Soh SM, Park GJ, Son TJ. Distribution of pathogenic vibrios in the aquatic environment adjacent to coastal areas of South Korea and analysis of the environmental factors affecting their occurrence. J Environ Health Sci. 2018; 44(2): 133-142.
10 Blake PA, Merson MH, Weaver RE, Hollis DG, Heublein PC. Disease caused by a marine Vibrio. Clinical characteristics and epidemiology. N Engl J Med. 1979; 300(1): 1-5.   DOI
11 Jung YS, Jung SI, Kang SJ, Jun CH, Park KH, Shin JH, et al. Clinical manifestations and prognostic factors in Vibrio vulnificus septicemia. Korean J Med. 2008; 74(3): 288-295.
12 Lee JM, Lee WJ, Kim MJ, Cho YS, Lee JS, Lee HJ, et al. Comparative evaluation of the VITEK 2 system and species-specific PCR methods for the detection of Vibrio species isolated from shrimp. J Food Hyg Saf. 2015; 30(3): 281-288.   DOI
13 Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4): 406-425.
14 Inoue Y, Miyasaka J, Ono T, Ihn H. The growth of Vibrio vulnificus and the habitat of infected patients in Kumamoto. Biosci Trends. 2007; 1(3): 134-139.
15 Liu JW, Lee IK, Tang HJ, Ko WC, Lee HC, Liu YC, et al. Prognostic factors and antibiotics in Vibrio vulnificus septicemia. Arch Intern Med. 2006; 166(19): 2117-2123.   DOI
16 Kim YH, Kwon D, Lee DH. Reorganization of national notifiable infectious diseases classification system. Public Health Wkly Rep. 2020; 13(1): 2-7.
17 Song JW, Jeong IH, Park SH, Yun SJ, Hur ES, Kim YS. Characterization of Vibrio vulnificus isolated from the west coastal area of Gyeonggi-do. Korean J Microbiol. 2021; 57(3): 197-203.   DOI
18 Shaw KS, Rosenberg Goldstein RE, He X, Jacobs JM, Crump BC, Sapkota AR. Antimicrobial susceptibility of Vibrio vulnificus and Vibrio parahaemolyticus recovered from recreational and commercial areas of Chesapeake Bay and Maryland Coastal Bays. PLoS One. 2014; 9(2): e89616.   DOI
19 Yoon YH, Park S, Kim JY, Lee YJ, Jeon DY, Choi GC, et al. Prevalence of toxin genes and profiles of antibitoc resistance in Vibrio vulnificus isolates from fish, fish tanks, and patients. J Food Hyg Saf. 2020; 35(1): 6-12.   DOI
20 Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing, 30th ed. Wayne: CLSI supplement M100; 2020.