Browse > Article
http://dx.doi.org/10.5668/JEHS.2020.46.6.667

Preventive Effect of Garlic Administration on Respiratory Toxicity Induced through Intratracheal Instillation of Fine Dust (PM10) in Rats  

Lee, YoonBum (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University)
Kim, GeunWoo (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University)
Song, YoungMin (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University)
Han, YoungHoon (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University)
Ha, ChangSu (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University)
Lee, JiSun (GLP Center, Daegu Catholic University)
Kim, MinHee (GLP Center, Daegu Catholic University)
Son, HyeYoung (GLP Center, Daegu Catholic University)
Lee, GiYong (GLP Center, Daegu Catholic University)
Heo, Yong (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University)
Kim, ChangYul (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University)
Publication Information
Journal of Environmental Health Sciences / v.46, no.6, 2020 , pp. 667-675 More about this Journal
Abstract
Objectives: Exposure to fine dust (PM10) could contribute to the occurrence of cardiovascular disease or respiratory abnormalities. Since garlic is known to possess an anti-oxidative stress effect, the present study was performed to evaluate the effect of garlic intake on fine dust-mediated pulmonary toxicity. Methods: Rats were intratracheally instilled with fine dust at 15 mg/kg body weight (BW)/day for five days following five-day intragastric intubation of garlic at 0.7 or 1.4 g/kgBW/day, or 13.1 mg/kgBW/day S-allyl-cysteine (SAC) as a reference component in garlic. Blood and bronchoalveolar lavage fluid (BALF) were collected. Results: Deposit of fine dust was visually and histopathologically observed in the lungs. Body weight gain during the instillation period was significantly lowered in all the groups instilled with fine dust. Neutrophil numbers in blood were significantly elevated in the fine dust alone group, but this alteration was diminished in the groups administered with garlic. Levels of serum glutathione were lower in the rats instilled with fine dust alone, and this decrease in the glutathione level seems dose-dependently compensated among the groups administered with garlic. Similar findings were observed in the BALF with statistical significance. Typical pulmonary histopathological observation related with inflammation was demonstrated in the lungs of the rats exposed to fine dust alone, whereas such histopathologic findings were not improved in the groups administered with garlic. Conclusion: The present study suggests that garlic intake could alleviate fine dust-mediated pulmonary or systemic toxicities. Further investigation is necessary to delineate the mechanism of garlic-mediated effects on pulmonary function.
Keywords
Fine dust instillation; garlic intake; glutathione; oxidative stress; pulmonary inflammation;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Desai, G, Schelske-Santos M, Nazario CM, Rosario-Rosado, RV, Mansilla-Rivera I, Ramirez-Marrero F, et al. Onion and Garlic Intake and Breast Cancer, a Case-Control Study in Puerto Rico. Nutr Cancer. 2020; 72(5): 791-800.   DOI
2 Hsieh, CC, Liu KF, Liu, PC, Ho YT, Li WS, Peng WH, et al. Comparing the Protection Imparted by Different Fraction Extracts of Garlic (Allium sativum L.) against Der p-Induced Allergic Airway Inflammation in Mice. Int J Mol Sci. 2019; 20(19): 4879.   DOI
3 Lee H. Heo JW, Kim AR, Kweon M, Nam S, Lim, JS. et al. Z-ajoene from Crushed Garlic Alleviates Cancer-Induced Skeletal Muscle Atrophy. Nutrients. 2019; 11(11): 2724.   DOI
4 Syed Mohammed Basheeruddin Asdaq. Antioxidant and hypolipidemic potential of aged garlic extract and its constituent, s-allyl cysteine, in rats. Evid Based Complement Alternat Med. 2015; 2015: 328545.
5 American Thoracic Society. Standards for the diagnosis and care of patients with chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 1995; 152(5 pt 2): S77-S121.
6 Pauwels RA, Buist AS, Calverley PM, Jenkins CR, Hurd SS. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease: NHLBI/WHO Global Initiative for Chronic Obstructive Lung Disease (GOLD) Workshop summary. Am J Respir Crit Care Med 2001; 163: 1256-1276.   DOI
7 Gross NJ. Extrapulmonary effects of chronic obstructive pulmonary disease. Curr Opin Pulm Med. 2001; 7(2): 84-92.   DOI
8 OECD Guidelines 403 for the testing of chemicals (Acute inhalation toxicity).
9 Agusti AG, Noguera A, Sauleda J, Sala E, Pons J, Busquets X. Systemic effects of chronic obstructive pulmonary disease. Eur Respir J. 2003; 21(2): 347-360.   DOI
10 Wouters EF, Creutzberg EC, Schols AM. Systemic effects in COPD. Chest. 2002; 121(5 Suppl): 127S-130S.   DOI
11 Osier M. Oberdorster G. Intratracheal inhalation vs intratracheal instillation: differences in particle effects. Fundam Appl Toxicol. 1997; 40(2): 220-227.   DOI
12 Mikkelsen L, Jensen KA, Koponen IK, Saber AT, Wallin H, Loft S, et al. Cytotoxicity, oxidative stress and expression of adhesion molecules in human umbilical vein endothelial cells exposed to dust from paints with or without nanoparticles. Nanotoxicology. 2013; 7(2): 117-134.   DOI
13 Jaffary SRA, Ahmed SW, Shakeel S, Asif HM, Usmanghani K. Acute and Sub Chronic Oral Toxicity Studies of Weight Loss Formulation in Experimental Animal Models. RADS J. Pharm. Pharm. Sci. 2017; 5(2): 8-12.
14 Baarends EM, Schols AM, Pannemans DL, Westerterp KR, Wouters EF. Total free living energy expenditure in patients with severe chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 1997; 155: 549-554   DOI
15 Ferreira IM, Brooks D, Lacasse Y, Goldstein RS. Nutritional support for individuals with COPD: a meta-analysis. Chest. 2000; 117(3): 672-678.   DOI
16 Brook RD, Franklin B, Cascio W, Hong Y, Howard G, Lipsett, M. et al. Air pollution and cardio-vascular disease: a statement for healthcare professionals from the Expert Panel on Population and Prevention Science of the American Heart Association. Circulation. 2004; 109(21): 2655-2671.   DOI
17 Foyer CH, Lelandais M. Kunert KJ. Photooxidative stress in plants. Physiologia Plantarum. 1994; 92(4): 696-717.   DOI
18 Gruhlke MC, Antelmann H, Bernhardt J, Kloubert V, Rink L, Slusarenko A J. The human allicin-proteome: S-thioallylation of proteins by the garlic defence substance allicin and its biological effects. Free Radic Biol Med. 2019; 131: 144-153.   DOI
19 Kim I, Park K, Lee K, Park M, Lim H, Shin H, et al. Application of various cytotoxic endpoints for the toxicity prioritization of fine dust (PM2.5) sources using a multi-criteria decision-making approach. Environ Geochem Health. 2020; 42: 1775-1788.   DOI
20 Zhang Y. Yang Z. Li R. Geng H. Dong C. Investigation of fine chalk dust particles' chemical compositions and toxicities on alveolar macrophages in vitro. Chemosphere. 2015; 120: 500-506.   DOI
21 Kim HH, Min G. Inhibitory effects of S-allylcysteine on cell proliferation of human cervical cancer cell line, HeLa. J Life Sci. 2015; 25: 397-405.   DOI
22 Korea Center for Disease control and Prevention. Development of Guideline for the preventive health damage caused by fine particle and yellow dust (Cardiovascular disease) Available: http://www.ndsl.kr/ndsl/commons/util/ndslOriginalView.do?dbt=TRKO&cn=TRKO201500014244&rn=&url=&page Code=PG18 [accessed at 15 may 2020].
23 Kodera Y, Matsuura H, Sumiyoshi H. Sumi SI. Garlic chemistry: chemical and biological properties of sulfur-containing compounds derived from garlic. Food Factors in Health Promotion and Disease Prevention. 2003; 30: 346-357.
24 Zarezadeh M. Baluchnejadmojarad T, Kiasalari, Z, Afshin-Majd S, Roghani M. Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: possible involved mechanisms. Eur J Pharmacol. 2017; 795: 13-21.   DOI
25 Dockery DW, Pope CA 3rd, Xu X, Spengler JD, Ware JH, Fay ME, et al. An association between air pollution and mortality in six U.S. cities. N Engl J Med. 1993; 329: 1753-1759.   DOI
26 Atkinson RW, Fuller GW, Anderson HR, Harrison RM, Armstrong B. Urban ambient particle metrics and health: A time series analysis. Epidemiology. 2010; 21: 501-511.   DOI
27 Loomis D, Grosse Y, Lauby-Secretan B, Ghissassi FE, Bouvard V, Benbrahim-Tallaa L, et al. The carcinogenicity of outdoor air pollution. Lancet Oncol. 2013; 14: 1262-1263.   DOI
28 Korea Center for Disease Control and Prevention. Development of guideline for the prevention and management of particulate matter/Asian dust particles-induced adverse health effect (Pulmonary diseases) Available: http://www.ndsl.kr/ndsl/commons/util/ndslOriginalView.do?dbt=TRKO&cn=TRKO201500014243&rn=&url=&pageCode=PG18 [accessed at 15 may 2020].
29 Anderson JO, Thundiyil JG, Stolbach A. Clearing the air: a review of the effects of particulate matter air pollution on human health. J Med Tox. 2012; 8(2): 166-175.   DOI
30 Sunil VR, Vayas KN, Fang M, Zarbl H, Massa C, Gow AJ, et al. World Trade Center (WTC) dust exposure in mice is associated with inflammation, oxidative stress and epigenetic changes in the lung. Exp Mol Patho. 2017; 102(1): 50-58.   DOI
31 Miller KA, Siscovick DS, Sheppard L, Shepherd K, Sullivan JH, Anderson GL, et al. Long-term exposure to air pollution and incidence of cardiovascular events in women. N Engl J Med. 2007; 356(5): 447-458.   DOI
32 Qian Z, Liang S, Yang S, Trevathan E, Huang Z, Yang R, et al. Ambient air pollution and preterm birth: a prospective birth cohort study in Wuhan, China. Int J Hyg Environ Health. 2016; 219(2): 195-203.   DOI
33 Shaikh SA, Tischer S, Choi EK, Fontana RJ. Good for the lung but bad for the liver? Garlic-induced hepatotoxicity following liver transplantation. J Clin Pharm Ther. 2017; 42(5): 646-648.   DOI
34 Berges R, Siess MH, Arnault I, Auger J, Kahane R, Pinnert MF, et al. Comparison of the chemopreventive efficacies of garlic powders with different alliin contents against aflatoxin B1 carcinogenicity in rats. Carcinogenesis. 2004; 25(10): 1953-1959.   DOI
35 Adaki S, Adaki R, Shah K, Karagir A. Garlic: Review of literature. Indian J Cancer. 2014; 51(4): 577-581.   DOI
36 Asemani, Y, Zamani N, Bayat M, Amirghofran Z. Allium vegetables for possible future of cancer treatment. Phytother Res. 2019; 33(12): 3019-3039.   DOI