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http://dx.doi.org/10.5668/JEHS.2019.45.5.520

Protective Effect of Albizzia julibrissin Leaf Extract on the Cytotoxicity Induced by Cupric Acetate Metallic Mordant  

Chung, Jung-Hwa (Sanbon Hospital, School of Medicine, Wonkwang University)
Rim, Yo-Sup (Department of Bioenvironmental Science, Sunchon National University)
Seo, Young-Mi (Department of Nursing, College of Medicine, Wonkwang Health Science University)
Publication Information
Journal of Environmental Health Sciences / v.45, no.5, 2019 , pp. 520-528 More about this Journal
Abstract
Objectives: This study assessed the cytotoxicity of the metallic mordant cupric acetate (CA) and the protective effect of Albizzia julibrissin (AJ) leaf extract on CA-induced cytotoxicity in NIH3T3 fibroblasts. Methods: For this study, cell viability and antioxidative effects such as the inhibitory activity of lipid peroxidation (LP) and superoxide anion-radical (SAR) scavenging activity were assessed. Results: CA significantly decreased cell viability in a dose-dependent manner, and the $XTT_{50}$ value was measured as $55.0{\mu}M$ of CA. The cytotoxicity of CA was determined as highly toxic by Borenfreund and Puerner's toxic criteria. The catalase antioxidant significantly increased cell viability diminished by CA-induced cytotoxicity in these cultures. Regarding the protective effect of AJ leaf extract on CA-induced cytotoxicity, AJ leaf extract remarkably increased the SAR scavenging ability and the inhibitory ability of LP. From these findings, it is suggested that oxidative stress is involved in the cytotoxicity of CA, and AJ leaf extract effectively protected CA-induced cytotoxicity via antioxidative effects. Conclusions: Natural resources like AJ leaf extract may be a putative therapeutic agent for treatment or alleviation of the toxicity induced by CA metallic mordant.
Keywords
Albizzia julibrissin; lipid peroxidation; metallic mordant; oxidative stress;
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