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http://dx.doi.org/10.5668/JEHS.2017.43.6.491

Metagenomic Analysis of Airborne Bacteria Community and Diversity in Gyeonggi-do, Korea, during March 2016, Asian Dust Event  

Jang, Jun Hyeong (Department of Life Science, Dongguk University-Seoul)
Kim, Ji Hye (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
Bae, Kyung-seon (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
Kim, Jeong Myeong (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
Lee, Won seok (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
Chung, Hyen-mi (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
Park, Sangjung (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
Seo, Taegun (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
Publication Information
Journal of Environmental Health Sciences / v.43, no.6, 2017 , pp. 491-498 More about this Journal
Abstract
Objective: Bacterial abundance and community compositions have been examined in Asian dust events, clarifying their impacts on public health. This study aims to determine the bacterial community compositions and viable bacteria in Asian dust particles in the Asian dust or non-Asian dust event of March 2016. Methods: The dust samples were collected using the high volume air sampler or high volume cascade impactor, and bacterial 16S rRNA genes were amplified using PCR, followed by pyrosequencing. Bacterial diversity index, richness estimate and community composition in the particles were analyzed from the sequencing data using Mothur software. Results: The results showed that the diversity and richness during Asian dust events were higher than them in non-Asian dust events. The total bacterial community analysis showed that at the phylum Proteobacteria, Actinobacteria and Firmicutes were the most dominant of Asian dust events and non-Asian dust events. In addition, the bacterial colony counts were higher during Asian dust event, comparing with non-Asian dust event. Conclusions: This study showed that bacterial community and richness of Asian dust samples was more complex and higher than non-Asian dust samples in Gyeonggi-do, Korea, which could affect public health and environment. Thus, the continuous monitoring of Asian dust could be an alternative for managing airborne bacteria.
Keywords
Asian Dust Event; Metagenomic; Airborne Bacteria; Bacterial Community;
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Times Cited By KSCI : 8  (Citation Analysis)
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