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http://dx.doi.org/10.5668/JEHS.2017.43.1.77

A Permeation Characteristics Study of Water- or Oil-soluble Substances through Condition Setting for the In Vitro Skin Absorption Method  

Seo, Ji-Eun (Department of Public Health, College of Natural Sciences, Keimyung University)
Lee, Jinho (Department of Chemistry, College of Natural Sciences, Keimyung University)
Kim, Bae-Hwan (Department of Public Health, College of Natural Sciences, Keimyung University)
Publication Information
Journal of Environmental Health Sciences / v.43, no.1, 2017 , pp. 77-86 More about this Journal
Abstract
Objectives: The purpose of this study was to compare permeation characteristics in three skin types using oil-soluble benzoic acid and water-soluble caffeine after method condition optimization based on OECD guideline 428. Methods: A Franz diffusion cell, a reliable alternative method for skin permeation, was used. One-milliliter samples were taken and immediately replaced with fresh solution in the receptor chamber at regular time intervals (1, 2, 4, 7, 10 and 24 hr). The amount of test substances was measured by LC-MS/MS. Results: The permeation rate increased dose-dependently, and the permeation orders were $KeraSkin^{TM}$ > hairless mouse full skin > human cadaver epidermis for skin types, and benzoic acid solution > caffeine solution > benzoic acid cream > caffeine cream for type of test materials. Conclusion: According to the definitions of Marzulli, benzoic acid and caffeine would be classified as 'fast' and 'moderate' compared with the permeation of other chemical species. The setting conditions and permeation characteristics performed in this study are expected to contribute to future permeation studies.
Keywords
Franz cell; skin permeation; alternative method; condition setting;
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