Browse > Article
http://dx.doi.org/10.5668/JEHS.2009.35.4.259

Protective Effects of Red Ginseng Saponins against to 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) Induced Toxicity in Guinea Pigs  

Hwang, Seock-Yeon (Department of Biomedical Laboratory Science, Daejeon University)
Lee, Chan-Yong (Department of Biomedical Laboratory Science, Daejeon University)
Publication Information
Journal of Environmental Health Sciences / v.35, no.4, 2009 , pp. 259-268 More about this Journal
Abstract
This study was carried out to investigate the protective effect of Red Ginseng Saponins on 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induced toxicities in guinea pigs ($200{\pm}10$ g). Normal control (NC) group guinea pigs ($200{\pm}10$ g) received vehicle and saline, while the TCDD-treated (TT) group was given water-extract (WE), saponin fraction (SF) and non-saponin fraction (NSF). Korean red ginseng fractions were administered from 1 week before TCDD-exposure for 4 weeks. Body weight loss and deteriorated clinical parameters related to sugar metabolism and liver function such as lipase and AST, respectively, these were significantly reduced by both saponin and non-saponin fractions. However, increase of lipase was attenuated by the saponin fraction in a dose-dependent manner. Only AST was affected by the saponin fraction. The results suggest that saponins are active substances in the Korean red ginseng water extract against TCDD induced toxicities in Guinea pigs.
Keywords
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD); Korean red ginseng; saponin; protective effect;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Pandelova, M., Stanev, I., Henkelmann, B., Lenoir, D., Schramm, K. W. : Correlation of PCDD/F and PCB at combustion experiments using wood and hospital waste. Influence of $(NH_4)_2SO_4$ as additive on PCDD/F and PCB emissions. Chemosphere, 75, 685-691, 2009   DOI   ScienceOn
2 Kim, K.-S., Lee, S. C., Kim, K.-H., Shim, W. J., Hong, S. H., Choi, K. H., Yoon, J. H., Kim, J.-G. : Survey on organochlorine pesticides, PCDD/Fs, dioxin-like PCBs and HCB in sediments from the Han river, Korea. Chemosphere, 75, 580-587, 2009   DOI   ScienceOn
3 Matsunaga, H., Katano, M., Yamamoto, H., Mori, M. : Potentiation of cytotoxic activity of mitomycin C by polyacetylenic alcohol, panaxytriol. Cancer Chemotherapy & Pharmacology, 33(4), 291-297, 1994   DOI
4 Yokozawa, T., Zhou, J. J., Hattori, M., Inaba, S. : Effect of ginseng in nephrectomized rats. Biological & Pharmaceutical Bulletin, 17(110), 1485-1489, 1994a   DOI   ScienceOn
5 Hook, G. E., Haseman, R. J. K., Lucier, G. W. : Induction and Suppression of hepatic and extrahepatic microsomal foreign compound metabolizing enzyme system by 2,3,7,8-TCDD. Chembio-biological Interaction, 10, 199-214, 1975   DOI   ScienceOn
6 Enan, E., Lui, C., Matsumura, F. : 2,3,7,8-TCDD causes reduction in Glucose uptake through Glucose Transports on the plasma membrane of the Guinea pig Adipocyte. Journal of Environment Science Health, B27(5), 495-510, 1992
7 Thomas, T., Han, K. K. : Mortality and morbidity among Army chemical corps Vietnam veterans: A preliminary report. The American Journal of Epidemiology, 18, 665-673, 1990
8 McConnel, E. E. : Acujte and chronic toxity. Carcinogenesis, Reproduction, Teratogenesis and Mutagenesis in Animals, Elsevier/North-Holland Biomedical Press. New York, pp. 241-266, 1990
9 Brewster, D. W., Bombick, D. W., Matsumura, F. : Rabbit serum hyperglycemia after administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Journal of Toxicology and Environmental Health, 25, 495-507, 1988   DOI   ScienceOn
10 Greig, J. B., Jones, G., Butler, W. H., Barnes, J. H. : Toxic effect 2,3,7,8-tetrachlorodibenzo-p-dioxin. Food and Cosmetics Toxicology, 11, 585-595, 1973   DOI   ScienceOn
11 Kirachi, R., Fleischer, K., Kamisaka, Arias, I. M. : Effect of renal function in the rat. The Journal of Clinical Investigation, 55, 1009-1015, 1975   DOI
12 Pegg, D. G., Hewitt, W. R., McCormack, K. M., Hook, J. B. : Effect 2,3,7,8-tetrachlorodibenzo-pdioxin on renal function in the rat. Journal of Toxicology and Enviromental Health, 2, 55-65, 1976   DOI   ScienceOn
13 Kaneko, H., Nakanishi, K., Murakami, A., Aidoh, H., Kuwashima, K. : The acute effects of massive dose of red ginseng on healthy adults under the condition of cold stress. Proceeding 96 Korea-Japan Ginseng Symposium, 22-30, 1996
14 Bowes, R. C., Parrish, A. R., Steinberg, M. A., Willett, K. L., Zhao, W., Savas, U., Jefcoate, C. R., Safe, S. H., Ramos, K. S. : Atypical cytochrome P450 induction profiles in glomerular mesangial cells at the mRNA and enzyme level. Evidence for CYP1A1 and CYP1B1 expression and their involvement in benzo[a]pyrene metabolism. Biochemical Pharmacology, 52, 587-595, 1996   DOI   ScienceOn
15 Zinkle, J. G., Vos, J. G., Moore, J. A., Gupta, B. N. : Hematologic and clinical chemistry effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin in laboratory animals. Environmental Health Perspectives, 9, 111-118, 1973   PUBMED
16 Yang, B. C., Li, Y. J., Chen, X. : Correlation between protective effect of ginsenosides against myocardial ischemia/reperfusion and lipid peroxidation in rats. Asia Pacific Journal Pharmacology, 4, 265-272, 1989
17 Kang, S. Y., Kim, S. H., Schini, V. B., Kim, N. D. : Dietary ginsenosides endothelium dependent relaxation in the thoraci arota of hypercholesterolemic rabbit. General Pharmacology, 26(3), 83-487, 1995   DOI   ScienceOn
18 Weissberg, J. B., Zinkl, J. G. : Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin on hematologic and blood coagulation function in the rat. Environment Health Perspectives, 5, 119-123, 1973   DOI   ScienceOn
19 Sergio, B. : Long term effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the peripheral nervous system. Neuroepidermiology, 7, 29-37, 1988   DOI
20 McGregor, D. B., Partensky, C., Wilbourn, J., Rice, J. M. : An IARC evaluation of polychlorinated dibenzop-dioxins and polychlorinated dibenzofurans as risk factors inhuman carcinogenesis. Environment Health Perspectives, 106(2), 755-760, 1998   DOI
21 Buu-Hoi, N. P., Harris, Moore, J. A., Vos, J. G. : Organism as target of 2,3,7,8-tetrachlorodibenzo-pdioxin(TCDD) intoxication. Naturwiss, 59, 174-175, 1972   DOI   ScienceOn
22 Park, H. J. : Effect of ginsenoside on the rat liver phosphorylation induced by carbonetetrachloride. Biochemical and Biophys Research Communication, 19(6), 834-838, 1996
23 Yokozawa, T., Inara, S. : Effect of ginseng in chronic renal failure rats induced by partial nephrectomy. The Ginseng Review, 18, 40-42, 1994b
24 Yang, J. B. : Studies on the Active Substances in Korean Red Ginseng against TCDD-induced Toxicity. Ph.D. Thesis, Daejeon University, Deajeon, Korea, 2002
25 Herrlich, P., Bohmer, F. D. : Redox regulation of signal transduction in mammalian cells. Biochemical Pharmacology, 59, 35-41, 2000   DOI   ScienceOn
26 Wang, I. C., Wu, Y. L., Lin, L. F., Chang-Chien, G. P. : Human dietary exposure to polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in Taiwan. Journal of Hazardous Materials, 164, 621-626, 2009   DOI   ScienceOn
27 Elma, Z. T., Ilian, E . Z., Christina, I. H. : Effect of ginsenoside Rg1 on insulin binding in mice liver and brain membranes. Phytotheraphy Research, 5(1), 46-48, 1991   DOI
28 Hassoun, E. A., Wilt, S. C., Van Birgelen, A., Alshrif, N. Z., Birnbaum, L. S., Stohs, S. J. : Induction of oxidative stress in brain of mice after subchronic exposure to 2,3,7,8-tetrachlorodibenzo-pdioxin. Toxicological Science, 42, 23-27, 1998   DOI
29 Huh, K., Jang, B. S., Park, J. M. : Protective effect of ginseng on brombenzene-induced hepatotoxicity in mice. Korea Journal of Ginseng Science, 12(2), 114-120, 1996
30 Faqi, A. S., Dalsenter, P. R., Merker, H.-J., Chahoud, I. : Reproductive toxicity and tissue concentration of low doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin in male offspring rats exposed throughout pregnancy and lactation. Toxicology and Applied Pharmacology, 150, 383-392, 1998   DOI   ScienceOn
31 Zinkle, J. G., Vos J. G., Moore, J. A., Gupta, B. N. : Hematologic and clinical chemistry effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin in laboratory animals. Environmental Health Perspectives, 9, 111-118, 1973   PUBMED
32 Gupta, B. N., Vos, I. G., Moore, J. A., Zinkl, J. G., Bullock, B. C. : Pathologic effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin in laboratory animals. Environment Health Perspectives, 9, 125-139, 1973
33 Zhuo, D. : Preventive Geriatrics : An overview from traditional Chinese medicine. The American Journal of Chinese Medicine, 10(1-4), 32-39, 1982   DOI   ScienceOn
34 Fang, Y. X., Shen, N., Chen, X. : Beneficial changes in prostacyclin and thromboxane A2 by ginsenoside in myocardial infection and repercussion injury of dogs. Acta Pharmacologica Sinica, 7(3), 226-230, 1986
35 Roman, B. L., Peterson, R. : In utero and lactational exposure of the male rats to 2,3,7,8-tetrachlorodibenzo-p-dioxin impairs prostate development. Toxicology and Applied Pharmacology, 150, 240-253, 1998   DOI   ScienceOn
36 Klotz, D. M., Beckman, B. S., Hill, S. M., McLachlan, J. A., Walters, M. R., Arnold, S. F. : Identification of environmental chemicals with estrogenic activity using a combination of in vitro assays. Environment Health Perspectives, 104, 1084-1089, 1996   DOI   ScienceOn
37 Joo, C. N., Lee, H. B. : Effect of ginseng saponin on LDL receptor biosynthesis. Proc. 5th International Ginseng Symposium, 47-53, 1988
38 Harris, M. W., Moore, J. A., Vos, J. G., Gupta, B. N. : General biological effect of TCDD in laboratory animals. Environment Health Perspectives, 9, 101-109, 1973   PUBMED
39 Brewster, D. W., Madhukar, B. V., Matsumura, F. : Influence of 2,3,7,8-TCDD on the protein composition of the plasma membrane of the hepatic cells from the rat. Biochemical and Biophys Research Communication, 107, 68-74, 1982   DOI   ScienceOn
40 Kim, S. W. : Improvement of crude ginseng saponin preparation method and biological activities of each ginsenoside. Ph.D. Thesis, Hanyang University Seoul, Korea, 1997
41 Michalek, J. E., Ketchum, N. S., Check, I. J. : Serum dioxin and immunologic responses in veterans of Operation Ranch Hand. The American Journal of Epidemiology, 149, 1038-1046, 1999   DOI   ScienceOn
42 Ann Aschengrau Richard R. Monson : Paternal military service in Vietnam and the risk of late adverse pregnancy outcome. The America Journal of Public Health, 80(10), 1218-1224, 1990   DOI   ScienceOn
43 Kita, T., Hata, T., Kawashima, Y., Kaku, T., Itoh, E. : Pharmacological a ctions of ginseng s aponin in s tress mice. Journal of Pharmacobio-Dynamics, 4, 381-400, 1981   DOI   PUBMED
44 Moore, J. A., Gupta, B. N., Vos, J. G. : Proceeding of the National Conference on Polychlorinated Biphenyls, Chicago, III., 77-80, 1976
45 Kim, N. W., Piatyszek, M. A., Prowse, K. R., Harley, C. B., West, M. D., Ho, P. L. C., Coviello, G. M., Wright, W. E., Weinirch, S. L., Shay, J. W. : Specific association of human telomerase activity with immortal cells and cancer. Science, 266, 2011-2015, 1994   DOI   ScienceOn
46 Gillis, C., Norman : Pulmonary vascular effects of ginsenosides. Proceeding 6th International Ginseng Symposium, 36-39, 1993
47 Eui, S. C., Kim, B. Y., Paik, T. H., Joo, G. N. : Effect of Ginseng on Alcohol Metabolism. Journal of Korean Biochemistry, 11(1), 1-6, 1978
48 Whitelock, J. P. : Induction of cytochrome P4501A1. Annual Review of Pharmacology and Toxicology, 39, 103-125, 1999   DOI   ScienceOn
49 Prieto, J. G., Femandez, E., Redondo, P. A., Ferrando, A., Vila, L., Estal del, J., Alvarez, A 1. : Korean red ginseng in exhaustion. Proceeding 6th International Ginseng Symposium, 171-178, 1993
50 Song, J. H., Park, M. J., Kim, E., Kim, Y. C. : Effect of Panax ginseng on galactosamine induced cytotoxicity in primary cultured rat hepatocytes. Yakhak Hoeji, 34(5), 341-347, 1990   ScienceOn
51 Song, J. H., Park, M. J., Kim, E., Kim, Y. C. : Effect of Panax ginseng on galactosamine induced cytotoxicity in primary cultured rat hepatocytes. Yakhak Hoeji, 34(5), 341-347, 1990   ScienceOn
52 Matsuda, H., Kubo, M., Mizuno, M. : Pharmacological study on Panax ginseng C.A. Meyer. Cardiovascular ef fcte o f red g inseng a nd white g inseng. Shoykugaku Zasshi, 41(12), 125-134, 1987
53 Tong, L. S., Chao, G. Y. : Effects fo Ginsenoside Rg1 of Panax ginseng on Mitosis in Human Blood Lymphocyte in vitro. The American Journal of Chinese Medicine, 8(3), 254-262, 1980   DOI   ScienceOn
54 Hwang, S.-Y. : Protective effect of Panax Ginseng on TCDD-induced Toxicity in Male Guinea Pig. Ph.D. Thesis, Hannam University, Daejeon, Korea, 1999
55 Yokozawa T, Inara : Effect of ginseng in chronic renal failure rats induced by partial nephrectomy. The Ginseng Review, 18, 40-42, 1994b
56 Brewster, D. W., Matsumura, F. : Differential effect of 2,3,7,8-tetrachlorodibenzo- p-dioxin on adipose tissue lipoprotein lipase activity in the guinea pig, rat, hamster, rabbit and mink. Comparative Biochemistry and Physiology, 93(1), 49-53, 1989
57 Yokozawa, T., Zhou, J. J., Hattori, M., Inaba, S. : Effect of ginseng in nephrectomized rats. Biological & Pharmaceutical Bulletin, 17(110), 1485-1489, 1994a   DOI   ScienceOn