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Oxidative Transformation of Triclosan by Laccase  

Kim, Young-Jin (Department of Civil Engineering and Applied Mechanics, McGill University)
Publication Information
Journal of Environmental Health Sciences / v.31, no.1, 2005 , pp. 61-65 More about this Journal
Abstract
The oxidative transformation of triclosan with laccase from Trametes versicolor was conducted in a closed, temperature controlled system containing phosphate buffer for pH control. The optimum pH for triclosan transformation showed about 5. Despite the observation that elevated temperatures tended to inactivate the enzyme, increased transformation of triclosan was observed up to $50^{\circ}C$. Of the mediators studied, ABTS was most successful at enhancing triclosan transformation. About 80% of the toxicity of the initial mixture was reduced after the enzymatic treatment. In the presence of 1.0 mM of anions such as sulfite, sulfide, and cyanide, triclosan transformation was greatly inhibited. Chloride and fluoride ions exhibited inhibition of triclosan transformation at 25 mM. Ferric ion substantially inhibited triclosan transformation at 1.0 mM.
Keywords
laccase; triclosan; 2,4,4'-trichloro-2'-hydroxydiphenyl ether; Trametes versicolor; antibiotic;
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