Browse > Article

Reaction Conditions for Laccase Catalyzed Degradation of Bisphenol A  

Kim, Young-Jin (Department of Civil Engineering and Applied Mechanics, McGill University)
Publication Information
Journal of Environmental Health Sciences / v.30, no.2, 2004 , pp. 79-83 More about this Journal
Abstract
The oxidative degradation of BPA with laccase from Trametes versiclor was conducted in a closed, temperature controlled system containing acetate buffer for pH control. The effects of medium pH, buffer concentration, temperature and mediator on degradation of BPA were investigated. The inactivation of the enzyme by temperature and reaction product was also studied. The optimal pH for BPA degradation showed about 5. Buffer concentration did not affect BPA degradation. On the other hand, the enzyme stability was higher at low concentration buffer(25 mM). Temperature rise increased the degradation rate of BPA up to 45$^{\circ}C$. The valuable mediator of laccase for BPA was ABTS. Elevated temperature and reaction product irreversibly inactivated the enzyme.
Keywords
bishenol A; 2,2-bis(4-hydroxyphenyl)propane; endocrine disrupting chemical; laccase; Trametes versicolor;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Perez, P., Pulgar, R., Olea-Serrano, F., Villalobos, M., Rivas, A., Metzler, M., Pedraza, V. and Olea, N. : The estrogenity of bisphenol A-related diphenylalkanes with various substituents at the cental carbon and hydroxy groups. Environ. Heatth Perspect., 106, 167-174, 1998   DOI   ScienceOn
2 Schafer, T., Lapp, C., Hanes, C., Lewis, J., Wataha, J. and Schuster, G. : Estrogenicity of bisphenol A and bisphenol A dimethacrylate in vitro. J. Biomed. Mater. Res., 45, 192-197, 1999   DOI   ScienceOn
3 Wblfenden, B. S. and Wilson, R. L.: Radical cations as reference chromogens in kinetic studies of one-electron transfer reactions: pulse radiolysis of 2,2'-azinobis-(3-ethylbenz-thiazoline-6-sulphonate). J. Chem. Perkin. Trans., 2, 805-812, 1982
4 Hirano, T., Honda, Y., Watanabe, T. and Kuwahara, M. : Degradation of bisphenol A by the lignin-degrading enzyme, manganese peroxidase, Produced by the white-rot basidiomycete, Pteumtus ostreatus. Biosci. Biotechnol Biochem., 64(9), 1958-1962, 2000   DOI   ScienceOn
5 Mol, H. G. J., Sunarto, S. and Steijger, O. M. : Determination of endocrine disruptors in water after derivatization with N-methyl-N-(tert-butyldimethiltri-fluoroacetamide) using gas chromatography with mass spectrometric detection. J. Chromtogr. A, 879, 97-112, 2000   DOI   ScienceOn
6 Okazaki, S., Michizoe, J., Goto, M., Furusaki, S., Wariishi, H. and Tanaka, H. : Oxidation of bisphenol A catalyzed laccase hosted in reverse micelles in organic media. Enzyme Microb. Technol., 31, 227-232, 2002   DOI   ScienceOn
7 Steinmetz, R., Mitchener, N. A., Grant, A., Allen, D. L., Bigsby, R. M. and Ben-Jonathan, N. : The xenoestrogen bisphenol A induces growth, differentation, and c-fos gene expression in the female reproductive tract. Endocrinotogy, 136, 2741-2747, 1998
8 Uchida, H., Fukuda, T., Miyamoto, H., Kawabata, T.. Takashima, Y., Suzuki, M. and Uwajima, T.: Degradadon of Bisphenol A by Purified Laccase from Trametes villosa. Biochem. Biophys. Res. Commun., 287, 355-358, 2001   DOI   ScienceOn
9 Gianfreda, L., Xu, F. and Bollag, M. : Laccases a useful group oxidoreductive enzymes. Bioremediation Journal, 3, 1-26, 1999   DOI   ScienceOn
10 Aitken, M. D. and Heck, P. E. : Tumover capacity of Coprinus cinereus peroxidase for phenol and monosubstituted phenols. BiotechnoI. Prog., 14, 487-492, 1998   DOI   ScienceOn
11 Barreca, A. M., Fabbrini, M. F., Galli, C., Gentili, P. and Ljunggren, S. : Laccase/mediated oxidation of a lignin model for improved delignification procedures. Joumal of Molecular Catalysis B: Enzymatic 26, 105-110, 2003   DOI   ScienceOn
12 Joshi, D. and Gold, M. : Degradation of 2,4,5-trichlorophenol by the lignin-degrading basidiomycete Phanemchaete chrysosporium. Appl. Envrin. Microbiol., 59, 1779-1785, 1993
13 Takaka, T, Tonosaki, T., Nose, M., Tomidokoro, N., Kadomura, N., Fujii, T. and Taniguchi, M. : Treatment of Model Soil Contaminated with Phenolic Endorine-Disrupting Chemicals with Laccase from Trametes sp. in a Rotating Reactor J. Biosci. Bioen., 92(4), 312-316, 2001   DOI
14 Akatas, N. and A. Tanyolac : Reaction conditions laccase-catalyzed polymerization of catechol. Bioresource Technotogy, 87, 209-214, 2003   DOI   ScienceOn
15 Levin, L., Viable, A. and Forchiassin, A. : Degradation of organic pollutants by white rot basidiomycete Trametes trogii. International Biodeterioration & Biodesradation, 52, 1-5, 2003   DOI   ScienceOn
16 AkataU, N., Kibarer, G. and Tanyolag, A.: Effects of reaction conditions laccase-catalyzed l-naphthol polymerization. J. Chem. Technol. Biotechnol., 75, 840-846, 2000   DOI   ScienceOn
17 Yaropolov, A. I., Skorobogatko, O. V, Vartanov, S. S. and Varfolomeyev, S. D. : Laccase properties, catalytic mechanism and applicability. Appt. Bioch. Biotech., 49, 257-279, 1994   DOI   ScienceOn
18 Chan, H. C., Holland, R. D., Bumpus, J. A., Churchwell, M. I. and Doerge, D. R. : Inactivation of Coprinus cinereus peroxidase by 4-chloroani1ine during turnover:compahson with horseradish peroxidase and bovine lactoperoxidase. Chem. Biol. Inter., 123, 197-217, 1999   DOI   ScienceOn
19 Yaver, D. S., Xu, F,, Golightly, E. J., Brown, K. M., Rey, M. W., Schneider, P., Halkier, T., Mondorf, K. and Dalboge, H. : Purification, characterization, molecular cloning, and expression of two laccase genes from the white rot basidiomycete Trametes villosa. Appl. Environ. Microbial., 62, 834-841, 1996
20 Fukuda, T., Uchida, H., Takashima, Y., Uwajima, T., Kawabata, T. and Suzuki, M. : Degradation of Bisphenol A by PuriSed Laccase from Trametes villosa. Biochem. Biophys. Res. Commun., 284, 704-706, 2001   DOI   ScienceOn