Browse > Article

Effect of Cyclohexanone Treatment on the Activities of Oxygen Free Radical Metabolizing Enzyme in the Liver Damaged Rats  

김현희 (계명대학교 자연과학대학 공중보건학과)
조현성 (계명대학교 자연과학대학 공중보건학과)
윤종국 (계명대학교 자연과학대학 공중보건학과)
Publication Information
Journal of Environmental Health Sciences / v.28, no.2, 2002 , pp. 81-88 More about this Journal
Abstract
Effect of cyclohexanone treatment on the activities oxygen free radical and cyclohexanone metabolizing enzyme in acute liver damaged rats, was investigated. Acute liver damage was induced in rats with pretreatment of 50% $CCl_4$ in olive oil(0.1ml/100g body wt) intraperitoneally 3 times every other day. Cyclohexanone(1.56g/kg body wt, i.p.) was administered to the animals 24 hours after the last Pretreatment of CC1$_4$. Rats were sacrificed at 4 hours after injection of cyclohexanone. On the basis of liver weight/body weight(％), serum levels alanine aminotransferase activity and hepatic protein content, cyclohexanone treatment to acute liver damaged animals led to the more enhanced liver damage. On the other hand, injection of cyclohexanone to the rats led to the increased activities of hepatic cytochrome P-450 dependent aniline hydroxylase and xanthine oxidase. Furthermore, by treatment of cyclohexanone to the acute liver damaged rats hepatic xanthine oxidase activity was more increased than the $CCl_4$ treated rats. In case of oxygen free radical scavenging system, the hepatic glutathione content and the activities of hepatic glutathione S-transferase, catalase, superoxide dismutase were generally increased by injection of cyclohexanone to rats, and the hepatic glutathione content, catalase and alcohol dehydrogenase activities were more decreased in liver damaged rats by the treatment of cyclohexanone. In conclusion, the cyclohexanone treatment to acute liver damaged rats led to enhancement of liver damage that may be due to oxygen free radical together with cyclohexanone.
Keywords
Liver Damaged Rats; Cyclohexanone; Cyclohexanone and Oxygen Free Radical Metabolizing Enzyme;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Lee, H.J.. Cho, H.G. and Yoon, C.G.: Effect of CCI$_4$-induced liver damage on the metabolism of xylene in rats. Kor. J. Env. Hlth. Soc., 25(1), 102-108, 1999
2 Deneke, S.M. and Fanburg, B.L.: Normobaric oxygen toxicity of the lung. N. Engl. J. Med., 303(2), 76-86, 1980   DOI   ScienceOn
3 Reitman, S. and Frankel, S.: A Colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am. J. Clin pathol., 28, 56-63, 1957   DOI
4 Bidlack, W.R. and Lowery, G.L.: Multiple drug metabolism:p-nitroanisole reversal of acetone enhanced aniline hydroxylation. Biochem. Pharmacol., 31(3), 311-317, 1982   DOI   ScienceOn
5 Yoon, C.G.: A modified colorimetric assay for xanthine oxidase in rat liver extracts. Keimyung Research Journal(Keimyung Junior College), 2, 295-308, 1984
6 Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J.: Protein measurement with the folin phenol reagent. J. Biol. Chem., 193, 265-275, 1951
7 Leibovitz, B.E. and Siegel, B.V.: Aspects of free radical reactions in biological system:aging. J. Gerontol., 35(1), 45-56, 1980   DOI   ScienceOn
8 Free, J.A.: Superoxide, superoxide dismutase and oxygen toxicity in 'Metal ion activation of dioxygen'(JG Sprio, ed), pp. 209-237, john Willy & Sons, INC., 1980
9 Bergmeyer, H.U.: Method of enzymatic analysis, Academic Press, New York, 2, 428-429, 1974
10 Habig, W.H., Pabst, M,J. and Jakoby, W.B.: Glutathione S-transferase. The first enzymatic step in mercapturic acid formation. J. Biol. Chem, 249(22), 7130-7139, 1974
11 한선일, 윤형원, 윤종국: 연령이 다른 흰쥐에 Bromobenzene 투여가 간손상에 미치는 영향. 대한의생명과학회지, 5(2), 201-208, 1999
12 Schefler, W.C.: Statistics for the biological sciences, pp. 84-89. Addison-Wesley, London, 1980
13 Gupta, .PK., Lawrence, W.H., Turner, J.E. and Autian, J.: Toxicological aspects of cyclohexanone. Taxicol. Appl. Pharmacol., 49(3), 525-533, 1979   DOI   ScienceOn
14 Karmen, A.: A note on the spectrophotometer assay of glutamic oxaloacetic transaminase in human blood serum. J. Clin Invest., 34, 131-133, 1955
15 Ong, C.N., Chia, S.E., Phoon, W.H., Tan, K.T. and Kok, P.W.: Monitoring of exposure to cyclohexanone through the analysis of breath and urine. Scand. J. Work Environ Health, 17(6), 430-435, 1991   DOI   ScienceOn
16 Lesko, S.A, Lorentzen, R.J. and Ts, o. P.O.: Role of superoxide in deoxyribonucleic acid strand scission. Biochemistry, 19(13), 3023-3028, 1980   DOI   ScienceOn
17 Freeman, B.A. and Crapo, J.D.: Biology of disease:free radicals and tissue injury. Lab Invest, 47(5), 412-426, 1982
18 Martin, J.P., Dailey, M. and Sugarman, E.: Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch Biochem. Biophy., 255(2), 329-336, 1987   DOI   ScienceOn
19 Cha, S.E. Yoon, C.G and Lee, S.l.: Effect of hepatic damaged rats on the toluene metabolism in carbon tetrachloride pretreated rats. J. Toxicol. Pab. Health. 14(3), 321-328, 1998
20 Fridovich, I. and Hassan, H.M.: Superoxide dismutase:detoxication of a free radical In 'Enzymatic basis of detoxication'(WB Jakoby, ed), p. 311, Academic Press, New York, 1980
21 Hafeman, D.G. and Hoekstra, W.G.: Protection aganist carbon tetrachloride-induced lipid peroxidation in the rat by dietary vitamin E, selenium, and methionine as measured by ethane evolution. J. Nutr., 107(4), 656-665, 1977.9) McCord, J.M. and Fridovich, I.: Superoxide dismutase:an enzymatic function for erythrocuprein(hemocuprein). J. Biol. Chem., 244, 6049-6055, 1967
22 Aebi. H.: Catalase in 'Methods of Enzymatic Analysis' (HU Bergmeyer, ed) Vol 2, pp. 673-684, Academic Press, New York, 1974
23 Simon, R.H., Scoggin, C.M. and Patterson, D.: Hydrogen peroxide causes the fetal injury to human fibroblasts exposed to oxygen radicals. J. Biol. Chem., 266(14), 7181-7186, 1981
24 Chow, C.K. and Tappel, A.L.: Response of glutathione peroxidase to dietary selenium in rats. J. Nutr., 104(4), 444-451, 1974
25 윤종국, 이미경, 이상일: 성장기간이 다른흰쥐에 사염화탄소 투여가 oxygen free radical 생성계 및 해독계 효소 활성에 미치는 영향. 한국노화학회지, 8(1), 35-42, 1998
26 Cha, S.E., Yoon, CG. and Lee, S.I.: Effect of hepatic damage on the toluene metabolism in CCl$_4$-pretreated rats. J. Taxicol. Pub. Health, 14(3), 321-328, 1998
27 Chae, S.N., Yoon, C.G. and Cho, H.G.: Effect of toluene application to skin on the enhancement of liver injury in CCl$_4$-pretreated rats. J. Biomed. Lab. Sci., 7, 79-83, 2001
28 Stirpe, F., Della Corte, E.: The regulation of rat liver xanthine oxidase. J. Biol. Chem., 244(14), 3855-3863, 1969
29 전태원: Cyclohexane에 의한 흰쥐의 폐 및 피부독성. 계명대학교 대학원 박사학위논문, 1999
30 Ellman, G.L.: Tissue sulfhydryl group. Arch Biochem. Biophys., 82, 70-77, 1959   DOI   PUBMED   ScienceOn
31 Elliott, T.H., Parke, D.U. and Williams, R.T.: Studies in detoxication. The metabolism of cyclo '$^1^4$C' hexane and its derivatives. Biochem. J., 72, 115-120, 1959   DOI
32 Perico, A., Cassinelli, C.B,. Brugnone, F., Bavazzano, P. and Perbellini, L.: Biological monitoring of occupational exposure to cyclohexane by urinary 1,2- and 1,4-cyclohexanediol determination. Int. Arch Occup. Environ Health, 72, 115-120, 1999   DOI   ScienceOn