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http://dx.doi.org/10.5620/eht.2012.27.e2012014

Lack of Mutagenicity Potential of Periploca sepium Bge. in Bacterial Reverse Mutation (Ames) Test, Chromosomal Aberration and Micronucleus Test in Mice  

Zhang, Mei-Shu (Biotoxtech. Co. Ltd.)
Bang, In-Seok (Department of Biological Science and the Research Institute for Basic Sciences, Hoseo University)
Park, Cheol-Beom (Fusion Research Laboratory, Hoseo University)
Publication Information
Environmental Analysis Health and Toxicology / v.27, no., 2012 , pp. 14.1-14.6 More about this Journal
Abstract
Objectives: The root barks of Periploca sepium Bge. (P. sepium) has been used in traditional Chinese medicine for healing wounds and treating rheumatoid arthritis. However, toxicity in high-doses was often diagnosed by the presence of many glycosides. The potential mutagenicity of P. sepium was investigated both in vitro and in vivo. Methods: This was examined by the bacterial reverse mutation (Ames) test using Escherichia coli WP2uvrA and Salmonella typhimurium strains, such as TA98, TA100, TA1535, and TA1537. Chromosomal aberrations were investigated using Chinese hamster lung cells, and the micronucleus test using mice. Results: P. sepium did not induce mutagenicity in the bacterial test or chromosomal aberrations in Chinese hamster lung cells, although metabolic activation and micronucleated polychromatic erythrocytes were seen in the mice bone marrow cells. Conclusions: Considering these results, it is suggested that P. sepium does not have mutagenic potential under the conditions examined in each study.
Keywords
Reverse mutation; Chromosome aberration; Micronucleus; Mice; Periploca sepium Bge;
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1 Mortelmans K, Zeiger E. The Ames Salmonella/microsome mutagenicity assay. Mutat Res 2000;455(1-2):29-60.
2 Zhang J, Ni J, Chen ZH, Li X, Zhang RJ, Tang W, et al. Periplocoside A prevents experimental autoimmune encephalomyelitis by suppressing IL-17 production and inhibits differentiation of Th17 cells. Acta Pharmacol Sin 2009;30(8):1144-1152.
3 Lu ZJ, Zhou Y, Song Q, Qin Z, Zhang H, Zhou YJ, et al. Periplocin inhibits growth of lung cancer in vitro and in vivo by blocking AKT/ERK signaling pathways. Cell Physiol Biochem 2010;26(4-5):609-618.
4 Zhao L, Shan B, Du Y, Wang M, Liu L, Ren FZ. Periplocin from Cortex periplocae inhibits cell growth and down-regulates survivin and c-myc expression in colon cancer in vitro and in vivo via beta-catenin/TCF signaling. Oncol Rep 2010;24(2):375-383.
5 Wang L, Yin ZQ, Zhang QW, Zhang XQ, Zhang DM, Liu K, et al. Five new C21 steroidal glycosides from Periploca sepium. Steroids 2011;76(3):238-243.
6 Joosten HF, van Acker FA, van den Dobbelsteen DJ, Horbach GJ, Krajnc EI. Genotoxicity of hormonal steroids. Toxicol Lett 2004;151(1):113-134.
7 Wang J, Liu H, Zhao J, Gao H, Zhou L, Liu Z, et al. Antimicrobial and antioxidant activities of the root bark essential oil of Periploca sepium and its main component 2-hydroxy-4-methoxybenzaldehyde. Molecules 2010;15(8):5807-5817.
8 Feng J, Zhang R, Zhou Y, Chen Z, Tang W, Liu Q, et al. Immunosuppressive pregnane glycosides from Periploca sepium and Periploca forrestii. Phytochemistry 2008;69(15):2716-2723.
9 Itokawa H, Xu J, Takeya K, Watanabe K, Shoji J. Studies on chemical constituents of antitumor fraction from Periploca sepium. II. Structures of new pregnane glycosides, periplocosides A, B and C. Chem Pharm Bull (Tokyo) 1988;36(3):982-987.
10 Itokawa H, Xu JP, Takeya K. Studies on chemical constituents of antitumor fraction from Periploca sepium. IV. Structures of new pregnane glycosides, periplocosides D, E, L, and M. Chem Pharm Bull (Tokyo) 1988;36(6):2084-2089.
11 Itokawa H, Xu JP, Takeya K. Studies on chemical constituents of antitumor fraction from Periploca sepium. V. Structures of new pregnane glycosides, periplocosides J, K, F and O. Chem Pharm Bull (Tokyo) 1988;36(11):4441-4446.
12 Wang L, Yin ZQ, Wang Y, Zhang XQ, Li YL, Ye WC. Perisesaccharides A-E, new oligosaccharides from the root barks of Periploca sepium. Planta Med 2010;76(9):909-915.
13 Zhu YN, Zhao WM, Yang YF, Liu QF, Zhou Y, Tian J, et al. Periplocoside E, an effective compound from Periploca sepium Bge, inhibited T cell activation in vitro and in vivo. J Pharmacol Exp Ther 2006;316(2):662-669.
14 Wan J, Zhu YN, Feng JQ, Chen HJ, Zhang RJ, Ni J, et al. Periplocoside A, a pregnane glycoside from Periploca sepium Bge, prevents concanavalin A-induced mice hepatitis through inhibiting NKT-derived inflammatory cytokine productions. Int Immunopharmacol 2008;8(9):1248-1256.
15 Zhang J, Shan BE, Li QM, Zhang C, Zhao RN, Ai J. Immunoregulatory effects of periplocin from Cortex Periplocae in tumor-bearing mice. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2009;25(10):887-890 (Chinese).
16 Jiangsu New Medical College. Dictionary of Chinese herbal medicines. Shanghai: Shanghai Scientific and Technologic Pressi; 1998, p. 1683, 2388 (Chinese).
17 Maron DM, Ames BN. Revised methods for the Salmonella mutagenicity test. Mutat Res 1983;113(3-4):173-215
18 Yahagi T, Nagao M, Seino Y, Matsushima T, Sugimura T. Mutagenicities of N-nitrosamines on Salmonella. Mutat Res 1977;48(2):121-129.
19 Ishidate M Jr, Odashima S. Chromosome tests with 134 compounds on Chinese hamster cells in vitro a screening for chemical carcinogens. Mutat Res 1977;48(3-4):337-353.
20 Heddle JA. A rapid in vivo test for chromosomal damage. Mutat Res 1973;18(2):187-190.
21 FuZhou Corona Science & Technology Developoment Co Ltd. Manufacture of hervals powders-plant powders extracts [cited 2008 Jan 1]. Available from: http://www.fzrm.com/plantextracts/Chinese_Silkvine_Root-bark_extract.htm.
22 MacGregor JT, Heddle JA, Hite M, Margolin BH, Ramel C, Salamone MF, et al. Guidelines for the conduct of micronucleus assays in mammalian bone marrow erythrocytes. Mutat Res 1987;189(2):103-112.
23 Phillipson JD. Phytochemistry and pharmacognosy. Phytochemistry 2007;68(22-24):2960-2972.
24 Yook CS. Periploca sepium bge. Seoul: Gyeonwon Press; 2001, p. 116 (Korean).
25 Flixya Entertainment LLC. Chinese silkwine root-bark [cited 2007 Oct 5]. Available from: http://www.flixya.com/blog/29445/Chinese-silkwine-Root-bark.
26 Tumor Research Center. Antitumor and immunoregulatory effects of active components from cortex periplocae [cited 2011 Nov 18]. Available from: http://www.tumorres.com/tumormarker/8114.htm.
27 Chu SS, Jiang GH, Liu WL, Liu ZL. Insecticidal activity of the root bark essential oil of Periploca sepium Bunge and its main component. Nat Prod Res 2012;26(10):926-932.