Browse > Article

Effect of Lavender Oil on the Ultraviolet-Damaged Mice Skin  

Rhie, Sung-Ja (Department of Beauty Design, Halla University)
Sim, Mi-Ja (Department of Beauty and Aesthetic Science, Gyeongbuk Provincial College)
Kim, Young-Chul (Department of Public Health, Keimyung University)
Publication Information
Environmental Analysis Health and Toxicology / v.23, no.4, 2008 , pp. 315-323 More about this Journal
Abstract
In this study, the backs with a hair cut of 6-week-old healthy ICR male mice were once exposed to a dose of $400\;mJ/cm^2$ UVB. An acute dermal inflammation was observed, and the certified 100% pure and natural lavender essential oil were applied to the UVB-exposed mice skin twice a day. It was observed that the mice exposed to UVB resulted in an acute inflammation, and when treated with lavender oil the degree of inflammation was much alleviated, and the inflamed skins of both the control and lavender oil-treatment groups were cured almost completely after 6 days of the UVB exposure. At 24 hours after UVB exposure, the epidermal keratinocytes in the control group showed a cell-membrane damage with the destruction of intercellular junctions, agglutination of tonofilaments within the cytoplasm and nucleus damage, while the lavender oil-treatment group had much less cell damage than the control group. While the control group showed a significant increase (p<0.05) in the activity of XO up to 144 hours, the lavender oil-treatment group did not show any significant increase except for 48 hours after the UVB exposure. Both the control and lavender essential oil-treatment groups had a significant decrease in the activities of CAT and SOD up to 96 hours. Particularly, the CAT activity was significantly lower(p<0.05) in the lavender oil-treatment group than the control group up to 48 hours, and higher than the control group at and after 96 hours. The GST activity was significantly decreased in both the control and lavender oil-treatment groups up to 96 hours after the UVB exposure except for the control group at 24 hours, and that of the lavender oil-treatment group was higher than the control group at and after 96 hours. Therefore, it is assumed that the application of the lavender oil to the ultraviolet-damaged mice skin can be effective in treatment for the damaged skin.
Keywords
UVB; lavender oil; mouse skin; inflammation; antioxidant enzyme;
Citations & Related Records
연도 인용수 순위
  • Reference
1 한경원, 한혜기, 명기범, 국홍일. 자외선 및 광화학 처치에 의한 흑색 마우스의 표피변화, 대한피부과학회지 1986;24: 586-593
2 Aebi H. Catalase. In: Methods of Enzymatic Analysis (Bergmeyer HU, eds.), Academic Press, New York 1974
3 Burns TS, Breathnach NC and Griffiths C. Rook's Textbook of Dermatology, Massachussets, Blackwell Science 2004
4 Harber LC and Bikers DR. Photosensitivity Diseases, 2nd ed. Philadelphia, WB Sunders 1989
5 Jager W, Buchbauer G, Jirovetz L and Fritzer M. Percutaneous absorption of lavender oil from a massage oil, J Soc Cosmetic Chem 1992; 43: 49-54
6 Lawless J. The illustrated encyclopedia of essential oils, New York, Element Books 1999
7 McGimpsey JA and Porter NG. Lavender, A Grower's Guide for Commercial Production, New Zealand, New Zealand Institute for Crop & Food Research Ltd 1999
8 Rivers JK, Norris PG, Murphy GM, Chu AC, Midgley G, Morris J, Morris RW, Yong AR and Hawk JLM. UVA Sunbeds: Tanning, photoprotection, acute adverse effects and immunological change, Br J Dermatol 1989; 120:767-777   DOI   ScienceOn
9 Urbach F. Evidence of epidermiology of UV-induced carcinogenesis in man, National Cancer Institute Monograph 1978; 50: 5-10
10 Kim HM and Cho SH. Lavender oil inhibits immediate-type allergie reaction in mice and rats, British Journal of Phamacology 1999; 51: 221-226
11 Soter NA. Acute effects of ultraviolet radiation on the skin, Seminars in Dermatology 1990; 9: 11-15
12 Reynolds ES. The use of lead citrate at high pH as electron opaque stain in electron miicroscopy, J Cell Biol 1963;17: 208-212   DOI   ScienceOn
13 Cavanagh HMA and Wilkinson JM. Biological activities of lavender essential oil, Phototherapy Research 2002; 16:301-308   DOI   ScienceOn
14 Martin JP, Dailey M and Sugarman E. Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation, Archives of Biochemistry and Biophysics 1987; 255: 329-336   DOI   ScienceOn
15 Mukhtra H and Elmets CA. Photocarcinogenesis: mechanisms, models and human health implications, Photochemistry and Photobiology 1996; 63: 355-360   DOI
16 Hruza LL and Pentland AP. Mechanisms of UV-induced inflammation, The Journal of Investigative Dermatology 1993; 100: 35-41   DOI   ScienceOn
17 McCord JM and Fridovich I. The biology and pathology oxygen radicals, Am Intern Med 1978; 89: 122-127   DOI   ScienceOn
18 Kumakiri M, Hashimoto K and Willis I. Biologic changes due to long-wave ultraviolet irradiation on human skin: Ultrastructural Study, The Journal of Investigative Dermatology 1977; 69: 392-400   DOI   ScienceOn
19 Stirpe F and Della CE. The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O), The Journal of Biological Chemistry 1969; 244: 3855-3863
20 Hawk JLM and Parrish JA. Responses of normal skin to ultraviolet radiation, In: Photoimmunology (Parrish JA, Kripke ML and Morrison WL, eds.), Plenum Medical Books, New York 1983
21 Paglia DE and Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutatione peroxidase, The Journal of Laboratory and Clinical Medicine 1967; 70: 158-169
22 Gerd O. Role of xanthine oxidase in thermal injury of skin, The American Journal of Pathology 1989; 35: 195-202
23 Deneke SM and Fanburg BL. Normobaric oxygen toxicity of the lung, The New England Journal of Medicine 1980;303: 76-86   DOI   ScienceOn
24 Pence BC and Naylor MF. Effect of single dose ultraviolet radiation on skin superoxide dismutase, catalase, and xanthine oxidase in hairless mice, J Invest Dermatol 1990;95: 213-216   DOI   ScienceOn
25 Watson ML. Staining of tissue sections for electron microscopy with heavy metals, J Biophysic Biochem Cytol 1958; 226: 475-479
26 Cotran RS and Pathak MA. The pattern of vascular leakage induced by monochromatic UV irradiation in rats, guinea pigs and hairless mice, J Invest Dermatol 1968; 1: 155-164
27 Gilchrest BA. Aging of skin, In: Dermatology in general medicine (Fitzpatrick TB, Eizen AZ, Wolff K, Freedberg IM and Austen KF, eds.), 4th edn. New York, McGraw-Hill 1993
28 Tisserand R and Balacs T. Essential oil safety, A Guide for Health Care Professionals, Harcourt Glasgow, 1999
29 Habig WH, Pabst MJ and Jakoby WB. Glutathione S-transferase. The first enzymatic step in mercapturic acid and formation, The Journal of Biological Chemistry 1974;249: 7130-7139
30 Leibovitz BE and Siegel BV. Aspects of free radical reaction in biological system, J Gerontol 1980; 35: 45-56   DOI   ScienceOn
31 Pathak MA, Riley FJ and Fitzpatrick TB. Melanin formation in human skin induced by long-wave ultraviolet and visible light, Nature 1982; 193: 148-150   DOI   ScienceOn
32 Miyachi Y, Imamura S and Niwa Y. Decreased skin superoxide dismutase activity by a single exposure of ultraviolet radiation is reduced by liposomal superoxide dismutase pretreatment, J Invest Dermatol 1987; 89: 111-112   DOI   ScienceOn