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Cadmium-induced E-cadherin Expression in Cerebrovascular Endothelial Cells  

Seok, Sun-Mi (Department of Physiology, Ajou University, School of Medicine)
Lee, Tae-Gu (Department of Physiology, Ajou University, School of Medicine)
Kim, Young-Chae (Department of Physiology, Ajou University, School of Medicine)
Moon, Chang-Hyun (Department of Physiology, Ajou University, School of Medicine)
Baik, Eun-Joo (Department of Physiology, Ajou University, School of Medicine)
Jung, Yi-Sook (Department of Physiology, Ajou University, School of Medicine)
Lee, Soo-Hwan (Department of Physiology, Ajou University, School of Medicine)
Publication Information
Environmental Analysis Health and Toxicology / v.22, no.2, 2007 , pp. 137-145 More about this Journal
Abstract
The effect of cadmium chloride $(CdCl_2)$ on the expression of E-cadherin was examined in bEnd.3 mouse brain endothelial cells. $CdCl_2$ induced $PGE_2$ release, which were blocked by non-steroidal antinflamatory drugs (NSAIDs) such as indomethacin and NS398 indicating the expression of COX-2 might contribute to $PGE_2$ production. $CdCl_2$ decreased the expression of E-cadherin, but not VE-cadherin at levels of mRNA and protein. Reduced expression level of E-cadherin was restored by NSAIDs, which was reversed by the addition of $PGE_2$. $CdC_2$-induced decrease of E-cadherin level was also recovered by antioxidants including N-acetylcyteine (NAC) and trolox. Together with previous report which showed $CdCl_2$ induced COX-2 expression in a cellular oxidative stress dependent manner, these data suggest that $CdCl_2$ decreases E-cadherin expression through induction of cellular oxidative stress and in turn COX-2 expression in brain endothelial cells.
Keywords
cadmium; brain endothelial cell; E-cadherin; oxidative stress; cyclooxygenase-2;
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