Browse > Article

Induction of Oxidative Stress by Silver Nanoparticles in Cultured Leydig Cells  

Park, Eun-Jung (College of Pharmacy, Dongduk Women's University)
Park, Kwang-Sik (College of Pharmacy, Dongduk Women's University)
Publication Information
Environmental Analysis Health and Toxicology / v.22, no.1, 2007 , pp. 57-64 More about this Journal
Abstract
Nanomaterials have been used to create unique devices at the nanoscale level. However, the toxicities of nanomaterials have not been fully tested and the risk of nanomaterials has been raised as an emerging issue in these days. In this study, the cytotoxicity of silver nanoparticles was tested using cultured mouse leydig cells. As results, silver nanoparticles showed cytotoxicity with the generation of reactive oxygen species (ROS). With the increased level of ROS, intracellular glutathione level was decreased. DNA fragmentation and caspase-3 activation suggested the apoptotic mechanism of cell death in leydig cells treated with silver nanoparticles.
Keywords
silver particles; leydig cells; cytotoxicity; oxidative stress;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Braydich-Stolle L, Hussain S, Schlager JJ and Hofmann MC. In vitro cytotoxicity of nanoparticles in mammalian germline stem cells, Toxicol Sci 2005; 88(2): 412-419   DOI   ScienceOn
2 Chen Y, Xue Z, Zheng D, Xia K, Zhao Y, Liu T, Long Z and Xia J. Sodium chloride modified silica nanoparticles as a non-viral vector with a high efficiency of DNA transfer into cells, Curr Gene Ther 2003; 3(3): 273-279   DOI
3 Choi JH, Kim JS, Kim YC, Kim YS, Chung NH and Cho MH. Comparative study of PM2.5-and PM10-induced oxidative stress in rat lung epithelial cell, J Vet Sci 2004; 5: 11-18
4 Danscher G and Stoltenberg M. Silver enhancement of quantum dots resulting from (1) metabolism of toxic metals in animals and humans, (2) in vivo, in vitro and immersion created zinc-sulphur/zinc-selenium nanocyrstals, (3) metal ions liberated from metal implants and particles, Prog Histochen Cytochem 2006; 41: 57-139   DOI   ScienceOn
5 Elbekai RH and El-Kadi AOS. The role of oxidative stress in the modulation of aryl hydrocarbon receptor-regulated genes by As3+, Cd2+, and Cr6+, Free Radic Biol Med 2005; 39: 1499-1511   DOI   ScienceOn
6 Elder A, Gelein R, Silva V, Feikert T, Opanashuk L, Carter J, Potter R, Maynard A, Ito Y, Finkelstein J and Oberdorster G. Translocation of inhaled ultrafine manganese oxide particles to the central nervous system, Environ Health Perspect 2006; 114(8): 1172-1178   DOI   ScienceOn
7 Lee D, Zhang W, Shirley SA, Kong X, Hellermann GR, Lockey RF and Mohapatra SS. Thiolated chitosan/DNA nanocomplexes exhibit enhanced and sustained gene delivery, Pharm Res 2007; 24(1): 157-167   DOI   ScienceOn
8 Park EJ and Park KS. Gene expression profiles of cultured rat cardiomyocytes (H9C2 cells) in response to arsenic trioxide at subcytotoxic level and oxidative stress, J Health Sci 2006; 52(5): 512-521   DOI   ScienceOn
9 Al-Jamal KT, Ruenraroengsak P, Hartell N and Florence AT. An intrinsically fluorescent dendrimer, as a nanoprobe of cell transport, J Drug Target 2006; 14(6): 405-412   DOI   ScienceOn
10 Cho SJ, Maysinger D, Jain M, Roder B, Hackbarth S and Winnik FM. Long-Term Exposure to CdTe Quantum Dots Causes Functional Impairments in Live Cells, Langmuir 2007; 23(4): 1974-1980   DOI   ScienceOn
11 Holsapple MP, Farland WH, Landry TL, Monteiro-Riviere NA, Carter JM, Walker NJ and Thomas KV. Research strategies for safety evaluation of nanomaterials, current challenges and data needs, Toxicological Sci 2005; 88(1): 12-17   DOI   ScienceOn
12 Kuhn KP, Chaberry IF, Masholder K, Stickler M, Benz VW, Sonntag HG and Erdinger L. Disinfection of surfaces by photocatalytic oxidation with titanium dioxide and UV A light, Chemosphere 2003; 5: 71-77   DOI   ScienceOn
13 Hussain SM, Hess KL, Gearhart JM, Geiss KT and Schlager JJ. In vitro toxicity of nanoparticles in BRL 3A rat liver cells, Toxicol in Vitro 2005; 19: 975-983   DOI   ScienceOn
14 Oberdorster G, Oberdorster E and Oberdorster J. Nanotoxicology. An emerging discipline evolving from studies of ultrafine particles, Environ Health Perspect 2005; 113: 823-829   DOI   ScienceOn
15 Bhattacharya J, Choudhuri U, Siwach O, Sen P and Dasgupta AK. Interaction of hemoglobin and copper nanoparticles: implications in hemoglobinopathy, Nanomedicine 2006; 2(3): 191-199   DOI   ScienceOn
16 Park KS. Toxicity of Nanomaterials and Strategy of Risk Assessment, Kor. J. Environ. Toxicol 2005; 20(4): 259-271   과학기술학회마을
17 Peters A, Veronesi B, Calderon-Garciduenas L, Gehr P, Chen LC, Geiser M and Reed W, Rothen-Rutishauser B, Schürch S and Schulz H. Translocation and potential neurological effects of fine and ultrafine particles a critical update, Part Fibre Toxicol 2006; 3: 13   DOI
18 Soto KF, Carrasco A, Powell TG, Murr LE and Garza KM. Biological effects of nanoparticulate materials, Mater Sci Eng C 2006; 26: 1421-1427   DOI   ScienceOn
19 Fotakis G, Cemeli E, Anderson D and Timbrell JA. Cadmium chloride-induced DNA and lysosomal damage in a hepatoma cell line, Toxicol in Vitro 2005; 19: 481-489   DOI   ScienceOn
20 Hsiao W, Mo ZY, Fang M, Shi XM and Wang F. Cytotoxicity of $PM_{2.5}\;and\;PM_{2.5-10}$ ambient air pollutants assessed by the MTT and the Comet assays, Mutation Research/Genetic Toxicol Environ Mutagen, 2000; 471: 45-55   DOI   ScienceOn
21 Hollinger MA. Toxicological aspects of topical silver pharmaceuticals. Crit Rev Toxicol 1996; 26(3): 255-260   DOI
22 Vasak M. Advances in metallothionein structure and functions, J Trace Elem Med Biol 2005; 19(1): 13-17   DOI   ScienceOn
23 Thomas K and Sayre P. Research strategies for safety evaluation of nanomaterials, part II: Toxicological Sci 2005; 87(2): 316-321   DOI   ScienceOn
24 Drake PL and Hazelwood KJ. Exposure-related health effects of silver and silver compound: a review, Ann Occup Hyg 2005; 49(7): 575-585   DOI   ScienceOn
25 Pierrat S, Zins I, Breivogel A and Sonnichsen C. Self-assembly of small gold colloids with functionalized gold nanorods, Nano Lett 2007; 7(2):259-263   DOI   ScienceOn
26 Oberdoster E. Manufactured nanomaterials (Fullerene, C60), induce oxidative stress in the brain of juvenile largemouth bass, Environ Health Perspect 2004; 112: 1058-1062   DOI   ScienceOn