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Effects of Subacute Oral Administration of Mancozeb on the Immunopathological Parameters and Splenocytes Proliferation in Mice  

Pyo Myoung-Yun (숙명여자대학교 약학대학)
Cheong Ae-Hee (서울특별시보건환경연구원)
Publication Information
Environmental Analysis Health and Toxicology / v.19, no.4, 2004 , pp. 367-373 More about this Journal
Abstract
Mancozeb, a polymeric complex of zinc and manganese salts of ethylene bisthiocarbamate (EBDC), is used widely in agriculture as fungicides, insecticides, and herbicides. Mancozeb can be occupationally and environmentally exposed to human and has been reported to induce estrogenic activity, therein it is considered as an endocrine disrupter. After female ICR mice were treated Mancozeb orally at the doses of 250, 1,000 and 1,500 mg/kg/day for consecutive 30 day, we investigated the effects of Mancozeb on the immunopathological parameters (body-, thymus-, spleen-, liver- and kidny-weight, splenic cellularity, hematological parameters) and mitogen (Con A, LPS)-induced splenocyte proliferation (SP). Liver- and kidney- weight were increased, but body- and thymus-weight, number of splenocytes and WBC were decreased, when compared with control group. When splenocytes isolated from the mice exposed to Mancozeb for 30 days were cultured in presence of mitogens, the SP against Con A was significantly and dose-dependently decreased and the SP against LPS was also slightly decreased. Our present results indicate that subacute exposure of Mancozeb to mice might show immunotoxic effect.
Keywords
Mancozeb; subacute exposure; immunopathology; splenocyte proliferation;
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1 Bindali BB and Kaliwal BB. Anti-implantation effect of a carbamate fungicide mancozeb in albino mice. Ind. Health 2002; 40(2): 191-197   DOI   ScienceOn
2 Hurley PM, Hill RN and Whiting RJ. Mode of Carcinogenic Action of Pesticides Inducing Thyroid Follicular Cell Tumors in Rodents. Environmental Health Perspectives 1998; 106(8): 437-445   DOI   PUBMED   ScienceOn
3 Kackar R, Srivastava MK and Raizada RB. Induction of gonadal toxicity to male rats after chronic exposure to mancozeb. Ind. Hea1th. 1997; 35 (l): 104-111   DOI   ScienceOn
4 Monis B and Valentich MA. Promoting effects of mancozeb on pancreas of nitrosomethylurea-treated rats. Carcinogenesis 1993; 14(5): 929-933   DOI   ScienceOn
5 Szepvolgyi J, Nagy K, Sajgone Vukan K, Regoly-Merei A, Soos K, Toth K, Pinter A and Antal M. Subacute toxicological examination of Dithane M-45. Fd. Chem. Toxic. 1989; 27(8): 531-538   DOI   ScienceOn
6 Trivedi N, Kakkar R, Srivastava MK, Mithal A and Raizada RB. Effect of oral administration of fungicide-manco-zeb on thyroid gland of rat. Ind. J. Exp. Biol. 1993; 31: 564-566
7 EPA US. Health Effects Test Guidelines-Pesticides and Toxic Substances. Immunotoxicity 1996
8 Varnagy L, Budai P, Molnar E, Takacs I and Fejes S. One-generation reproduction toxicity study of mancozeb and lead acetate. Meded. Rijksuniv. Gent. Fak. Landbou-wkd. Toegep. Biol. Wet. 2001; 66(2b): 873-878
9 Colosio C, Barcellini W, Maroni M, Alcini D, Bersani M, Cavallo D, Galli A, Meroni P, Pastorelli R, Rozzardi GP, Soleo L and Foa V. Immunomodulatory effects of Occupational exposure to Mancozeb. Arch. Environ. Health 1996; 51(6): 445-451   DOI   ScienceOn
10 Kackar R, Srivastava MK and Raizada RB. Studies on Rat Thyroid after Oral Administration of Mancozeb: Morphological and Biochemical Evaluations. Journal of Applied Toxicology 1997; 17(6): 369-375   DOI   ScienceOn
11 Rakich DR, Wataha JC, Lefebvre CA and Weller RN. Effects of dentin bonding agents on macrophage mitochondrial activity. J. Endodon. 1998; 24: 528-533   DOI   ScienceOn
12 Guest IC, Paik NW, Ryu JC, Park JS and Chang IM. Hematological values in normal laboratory mice (ICR and ddY strains). Kor. J. Toxicol. 1991; 7: 47
13 Larsson KS, Arnander C, Cekanova E and Kjellberg M. Studies of teratogenic effects of the dithiocarbamates Maneb, Mancozeb, and Propineb. Teratology 1976; 14: 171-184   DOI   ScienceOn
14 Harris ML, Chora L, Bishop CA and Bogart JP. Species-and age-related differences in susceptibility to pesticide exposure for two Amphibians, Rana pipiens, and Bufo americanus. Bull. Environ. Contam. Toxicol. 2000; 64: 263-270   DOI   ScienceOn
15 Subramoniam A, Agrawal D, Srivasrava SP and Seth PK. Influence of mancozeb on mitogenically responsive lipids in rat cerebrum and liver. Indian Journal of Experimental Biology 1991; 29: 943-945
16 Kackar R, Srivastava MK. and Raizada RB. Assessment of toxicological effects of mancozeb in male rats after Chronic exposure. Indian J. Exp. Biol. 1999; 37(6): 553-559
17 Baligar PN and Kaliwal BB. Induction of gonadal toxicity to female rats after chronic exposure to mancozeb. Ind. Health 2001; 39(3): 235-243   DOI   ScienceOn
18 Mahadevaswami MP, Jadaramkunti UC, Hiremath MB and Kaliwal BB. Effect of mancozeb on ovarian Compensatory hypertrophy and biochemical constituents in hemicastrated albino rat. Reproductive Toxicology 2000; 14: 127-134   DOI   ScienceOn
19 Mehrotra NK, Kumar S and Yogeshwer S. Enhancement of tumor-initiating activity of DMBA by the carbamate fungicide Mancozeb. Bull. Environ Contam. Toxicol. 1990; 44: 39-45   DOI   ScienceOn
20 Belpoggi F, Soffritti M, Guarino M, Lamvertini L, Cevolani D and Maltoni C. Results of long-term experimental studies on the carcinogenecity of ethylene-bis-dithio-carbamate (Mancozeb) in rats. Ann. N Y Acad. Sci. 2002; 982: 123-136   DOI
21 Chung AH and Pyo MY. Effects of acute administeration of mancozeb on the immune function in mice, Yakhak Hoeji 2004; 48(1): 41-46
22 농약연보. 농약공업협회. 1999; P.62-63