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Changes in Cellular Viability and Peroxidase Activities of Green Algae Selenastrum capricornutum (Chlorophyceae) to Cadmium  

Choi Eun-Joo (경희대학교 생물학과)
Lee Sang-Goo (경희대학교 생물학과)
Lee Seung-Jin (경희대학교 생물학과)
Moon Sung-Kyung (경희대학교 생물학과)
Park Yong-Seok (경희대학교 생물학과)
Rhie Ki-tae (경희대학교 생물학과)
Publication Information
Environmental Analysis Health and Toxicology / v.18, no.4, 2003 , pp. 295-303 More about this Journal
Abstract
Physiological cellular activities responses to cadmium (Cd) exposure in green algae with several reductases activities and viability of the cell were examined. The cell division of green algae, Selenastrum capricornutum treated with 5ppm was significantly decreased than that of normal algae. The mean cell number of normal algal culture was as twice much as than that of algae at 6 days after Cd treatment. The cellular viability of algae was analysed by flow-cytometry with fluorescent dye after esterase reaction on cell membrane. The 85.35% of cellular viability of normal culture was decreased to 34.35% when algae was treated with 5 ppm of Cd at 6 days after treatment. It was considered that those method of flow-cytometry is useful tool for toxicity test on micro-organisms in the respect of identifying cellular viability. Also, the activities of both glutathione peroxidase (GPX) and ascorbate peroxidase (APX), which are indirectly react against oxidative stress through reduction of glutathione by Cd were significantly increased with 25%. It is considered that both GPX and APX are involved in the metabolic pathway of Cd -detoxification with similar portion in Selenasturm capricornutum.
Keywords
cadmium toxicity; green algae; cellular vitality; peroxidase activities;
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