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In vitro Screening of UVA Phototoxicity Inhibitors using the Natural Products  

김현진 (한국화학 연구원 부설 안전성평가연구소)
김봉희 (충남대학교 약학대학)
Publication Information
Environmental Analysis Health and Toxicology / v.17, no.3, 2002 , pp. 253-259 More about this Journal
Abstract
The phototoxicity inhibitory activity of 15 natural products having antiinflammatory effect was screened by three in vitro methods: yeast growth inhibition test with Candida albicans, RBC photohemolysis and MTT assay. We induced phototoxic reaction by irradiating UVA (365 nm) on chlorpromazine (CPZ) that has been widely documented as phototoxic agent in clinical and experimental studies and then observed the effects of the natural products after treating them with CPZ. In yeast growth inhibition test, X. stramonium showed the inhibitory effect on the UVA phototoxicity and E. officinalis, Yeast, P. suffruticosa showed phototoxicity inhibitory effect in that their ％ hemolysis compared with control were 36.14${\pm}$ 2.69, 42.82${\pm}$1.35, 36.41${\pm}$0.48 on UVA. In MTT assay, all tested natural products increased cell viability compared with the control.
Keywords
UVA phototoxicity inhibitors; Natural products; Yeast growth inhibition; RBC photohemolysis; MTT assay; Chlorpromazine;
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