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http://dx.doi.org/10.11614/KSL.2021.54.4.265

Toxicological Assessment to Environmental Stressors Using Exoskeleton Surface Roughness in Macrophthalmus japonicus: New Approach for an Integrated End-point Development  

Park, Kiyun (Fisheries Science Institute, Chonnam National University)
Kwak, Ihn-Sil (Fisheries Science Institute, Chonnam National University)
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Abstract
Intertidal mud crab (Macrophthalmus japonicus) is an organism with a hard chitinous exoskeleton and has function for an osmotic control in response to the salinity gradient of seawater. Crustacean exoskeletons change in their natural state in response to environmental factors, such as changes in the pH and water temperature, and the presence of pollutant substances and pathogen infection. In this study, the ecotoxicological effects of irgarol exposure and heavy metal distribution were presented by analyzing the surface roughness of the crab exoskeleton. The exoskeleton surface roughness and variation reduced in M. japonicus exposed to irgarol. In addition, it was confirmed that the surface roughness and variation were changed in the field M. japonicus crab according to the distribution of toxic heavy metals(Cd, Pb, Hg) in marine sediments. This change in the surface roughness of the exoskeleton represents a new end-point of the biological response of the crab according to external environmental stressors. This suggests that it may affect the functional aspects of exoskeleton protection, support, and transport. This approach can be utilized as a useful method for monitoring the aquatic environment as an integrated technology of mechanical engineering and biology.
Keywords
exoskeleton surface roughness; biological end-point; toxicity assessment; pollutant exposure; integrated bio-indicator;
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1 Park, K., T.S. Kwak and I.S. Kwak. 2014. Vitellogenin gene characterization and expression of Asian paddle crabs (Charybdis japonica) following endocrine disrupting chemicals. Ocean Science Journal 49: 127-135.   DOI
2 Prosser, C.L., S.W. Green and T.S. Chow. 1955. Ionic and osmotic concentrations in blood and urine of Pachygrapsus crassipes acclimated to different salinities. Biology Bulletin 109: 99-107.   DOI
3 Robohm, R., A. Draxler, D. Wieczorek, D. Kapareiko and S. Pitchford. 2005. Effects of environmental stressors on disease susceptibility in American lobster: a controlled laboratory study. Journal of Shellfish Research 24: 773-780.   DOI
4 Sanchez, C., H. Arribart and M.M. Giraud-Guille. 2005. Biomimetism and bioinspiration as tools for the design of innovative materials and systems. Nature Materials 4: 277-288.   DOI
5 Sapozhnikova, Y., E. Wirth, K. Schiff and M. Fulton. 2013. Antifouling biocides in water and sediments from California marinas. Marine Pollution Bulletin 69: 189-194.   DOI
6 Vincent, J.F.V. 2002. Arthropod cuticle: a natural composite shell system. Composites A33: 1311-1315.   DOI
7 Chen, P.Y., A.Y.M. Lin, J. McKittrick and M.A. Meyers. 2008. Structure and mechanical properties of crab exoskeletons. Acta Biomaterialia 4: 587-596.   DOI
8 Gagne, F., C. Blaise and J. Pellerin. 2005. Altered exoskeleton composition and vi- tellogenesis in the crustacean Gammarus sp. Collected at polluted sites in the Saguenay Fjord, Quebec, Canada. Environmental Research 98: 89-99.   DOI
9 Glenn, R. and T. Pugh. 2006. Epizootic shell disease in American lobster (Homarus americanus) in Massachussetts coastal waters: interactions of temperature, maturity and intermolt duration. Journal of Crustacean Biology 26: 639-645.   DOI
10 Kim, S.U., T.R. Kim, E.S. Lee, M.S. Kim, C.K. Kim, L.R. Kim and G.Y. Shin. 2015. Formaldehyde and heavy metal migration from rubber and metallic packaging/utensils in Korea. Food Additives & Contaminants: Part B Surveillance 8: 7-11.   DOI
11 Kim, W.S., C. Hong, K. Park and I.S. Kwak. 2019. Ecotoxicological response of Cd and Zn exposure to a field dominant species, Chironomus plumosus. Korean Journal of Ecology and Environment 52: 266-273.   DOI
12 Liu, Z., S. Pan, Z. Sun, R. Ma, L. Chen, Y. Wang and S. Wang. 2015. Heavy metal spatial variability and historical changes in the Yangtze River estuary and North Jiangsu tidal flat. Marine Pollution Bulletin 98: 115-129.   DOI
13 Luft, A., M. Wagner and T.A. Ternes. 2014. Transformation of biocides irgarol and terbutryn in the biological waste water treatment. Environmental Science & Technology 48: 244-254.   DOI
14 Park, K., T.S. Kwak, W.S. Kim and I.S. Kwak. 2019. Changes in exoskeleton surface roughness and expression of chitinase genes in mud crab Macrophthalmus japonicus following heavy metal differences of estuary. Marine Pollution Bulletin 138: 11-18.   DOI
15 Walters, D.M., E. Rosi-Marshall, T.A. Kennedy, W.F. Cross and C.V. Baxter. 2015. Mercury and selenium accumulation in the Colorado River food web, Grand Canyon, USA. Environmental Toxicology and Chemistry 34: 2385-2394.   DOI
16 Zhao, S., C. Feng, W. Quan, X. Chen, J. Niu and Z. Shen. 2012. Role of living environments in the accumulation characteristics of heavy metals in fishes and crabs in the Yangtze River Estuary, China. Marine Pollution Bulletin 64: 1163-1171.   DOI
17 Mali, B., F. Mohrlen, M. Frohme and U. Frank. 2004. A putative double role of a chitinase in a cnidarian: pattern formation and immunity. Developmental & Comparative Immunology 28: 973-981.   DOI
18 ANSI (American National Standards Institute). 1986. Surface texture (surface roughness, waviness and lay). The American Society of Mechanical Engineers 4-10.
19 Park, K., C. Nikapitiya, W.S. Kim, T.S. Kwak and I.S. Kwak. 2016. Changes of exoskeleton surface roughness and expression of crucial participation genes for chitin formation and digestion in the mud crab (Macrophthalmus japonucus) following the antifouling biocide irgarol. Ecotoxicology and Environmental Safety 132: 186-195.   DOI
20 Kitaura, J., M. Nishida and K. Wada. 2002. Genetic and behavioral diversity in the Macrophthalmus japonicus species complex (Crustacea: Brachyura: Ocypodidae). Marine Biology 140: 1-8.   DOI
21 Luo, J., S. Pei, W. Jing, E. Zou and L. Wang. 2015. Cadmium inhibits molting of the freshwater crab Sinopotamon henanense by reducing the hemolymph ecdysteroid content and the activities of chitinase and N-acetyl-β-glucosaminidase in the epidermis. Comparative Biochemistry and Physiology Part C: Toxicolology & Pharmacolology 169: 1-6.   DOI
22 Luo, W., Y. Lu, T. Wang, P. Kong, W. Jiao, W. Hu, J. Jia, J.E. Naile, J.S. Khim and J.P. Giesy. 2013. Environmental concentrations and bioaccumulations of cadmium and zinc in coastal watersheds along the Chinese Northern Bohai and Yellow Seas. Environmental Toxicology and Chemistry 32: 831-840.   DOI
23 Mok, J.S., H.D. Yoo, P.H. Kim, H.D. Yoon, Y.C. Park, T.S. Lee, J.Y. Kwon, K.T. Son, H.J. Lee, K.S. Ha, K.B. Shim and J.H. Kim. 2015. Bioaccumulation of heavy metals in oysters from the southern coast of Korea: assessment of potential risk to human health. Bulletin of Environmental Contamination and Toxicology 94: 749-755.   DOI
24 Otania, S., Y. Kozukib, R. Yamanakab, H. Sasaokac, T. Ishiyamac, O. Yoshihito, H. Sakaic and Y. Fujiki. 2010. The role of crabs(Macrophthalmus Japonicus) burrows on organic carbon cycle in estuarine tidal flat, Japan. Estuarine, Coastal and Shelf Science 86: 434-440.   DOI
25 Cho, J., S. Hyun, J.H. Han, S. Kim and D.H. Shin. 2015. Historical trend in heavy metal pollution in core sediments from the Masan Bay, Korea. Marine Pollution Bulletin 95: 427-432.   DOI
26 Herman, P.M.J., J.J. Middelburg, J. Van de Koppel and C.R. Heip. 1999. Nutrients in estuaries, p. 195-240. In: Advances in ecological research: Estuaries (Nedwell, D.B. and D.G. Raffaelli, eds.). Academic Press, San Diego, CA.
27 Melnick, C.A., Z. Chen and J.J. Mecholsky Jr. 1996. Hardness and toughness of exoskeleton material in the stone crab, Menippe mercenaria. Journal of Materials Research 11: 2903-2907.   DOI