Browse > Article
http://dx.doi.org/10.11614/KSL.2021.54.3.257

Application of NGS Analysis for the Food Source of Bivalve  

Heo, Yu-Ji (Department of Environmental Education, Sunchon National University)
Jo, Hyunbin (Institute for Environment and Energy, Pusan National University)
Jung, Eunsong (Department of Integrated Biological Science, Pusan National University)
Kim, Hyun-Woo (Department of Environmental Education, Sunchon National University)
Publication Information
Abstract
In this study, we analyzed the food components in the release product that sampled Sinonovacula constricta from the foreshore littoral at Byeongnyang-myeon, Suncheon Bay. We used microscopy and next-generation sequencing (NGS) to evaluate the applicability of morphological and molecular methods to analyze release products. The higher species diversity observed in the NGS method is due to the different levels of species identification, as microscopy displays morphological and anatomical levels of plankton species identification in S. constrita. Moreover, NGS can identify the level of species in the organic matter by using the 18s_V9 primer.
Keywords
NGS; microscopy; Sinonovacula constricta;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Davenport, J., D. Ezgeta-Balic, M. Peharda, S. Skejic, Z. Nincevic-Gladan and S. Matijevic. 2011. Size-differential feeding in Pinna nobilis L. (Mollusca: Bivalvia): Exploitation of detritus, phytoplankton and zooplankton. Estuarine Coastal and Shelf Science 92: 246-254.   DOI
2 Kharlamenko, V.I., S.I. Kiyashko, A.B. Imbs and D.I. Vyshkvartzev. 2001. Identification of food sources of invertebrates from the seagrass Zostera marina community using carbon and sulfur stable isotope ratio and fatty acid analyses. Marine Ecology Progress Series 220: 103-117.   DOI
3 Lehane, C. and J. Davenport. 2006. A 15-month study of zooplankton ingestion by farmed mussels (Mytilus edulis) in Bantry Bay, Southwest Ireland. Estuarine Coastal and Shelf Science 67: 645-652.   DOI
4 Li, W., L. Fu, B. Niu, S. Wu and J. Wooley. 2012. Ultrafast clustering algorithms for metagenomic sequence analysis. Briefings in Bioinformatics 13(6): 656-668.   DOI
5 Jo, H., D.K. Kim, K. Park and I.S. Kwak. 2019. Discrimination of spatial distribution of aquatic organisms in a coastal ecosystem using eDNA. Applied Sciences 9(17): 3450.   DOI
6 Wong, W.H. and S.G. Cheung. 2001. Feeding rhythms of the green-lipped mussel, Perna viridis(Linnaeus, 1758) (Bivalvia: Mytilidae) during spring and neap tidal cycles. Journal of Experimental Marine Biology and Ecology 257(1): 13-36.   DOI
7 Deagle, B.E., D.J. Tolli, S.N. Jarman, M.A. Hindell, A.W. Trites and N.J. Gales. 2005. Molecular scatology as a tool to study diet: analysis of prey DNA in scats from captive Steller sea lions. Molecular Ecology 14(6): 1831-1842.   DOI
8 Dubois, S., H. Blanchet, A. Garcia, M. Masse, R. Galois, A. Gremare and N. Savoye. 2014. Trophic resource use by macrozoobenthic primary consumers within a semi-enclosed coastal ecosystem: stable isotope and fatty acid assessment. Journal of Sea Research 88: 87-99.   DOI
9 Feng, B., L. Dong, D. Niu, S. Meng, B. Zhang, D. Liu and J. Li. 2010. Identification of immune genes of the Agamaki clam (Sinonovacula constricta) by sequencing and bioinformatic analysis of ESTs. Marine Biotechnology 12(3): 282-291.   DOI
10 Foged, N. 1978. Diatoms in eastern Australia. Bibliotheca Phycologica 41: 1-243, 48 pls.
11 Kim, H.S. 2016. Phytoplankton variability in digestive tract of the Manila clam Ruditapes philippinarum in Gomso Bay, west coast of Korea. The Korean Journal of Malacology 32(3): 165-174 (in Korean).   DOI
12 Cassie, V. 1989. A contribution to the study of New Zealand diatoms. Bibliotheca Diatomologica 17: 1-266, 13 pls.
13 Koh, C.H., M.R. Yang and W.K. Chang. 1997. Growth and production of Sinonovacula constricta (Bivalvia) from the Hwaseong tidal flat in the Namyang Bay, Korea. The Sea 2(1): 21-30 (in Korean).
14 Yoon, T.H., H.E. Kang, C.K. Kang, S.H. Lee, D.H. Ahn, H. Park and H.W. Kim. 2016. Development of a cost-effective metabarcoding strategy for analysis of the marine phytoplankton community. PeerJ 4: e2115.   DOI
15 Zhang, Z., S. Schwartz, L. Wagner and W. Miller. 2000. A greedy algorithm for aligning DNA sequences. Journal of Computational Biology 7(1-2): 203-214.   DOI
16 Albaina, A., M. Aguirre, D. Abad, M. Santos and A. Estonba. 2016. 18S rRNA V9 metabarcoding for diet characterization: a critical evaluation with two sympatric zooplanktivorous fish species. Ecology and Evolution 6(6): 1809-1824.   DOI
17 Alfaro, A.C. 2006. Evidence of cannibalism and bentho-pelagic coupling within the life cycle of the mussel, Perna canaliculus. Journal of Experimental Marine Biology and Ecology 329(2): 206-217.   DOI
18 Amaral-Zettler, L.A., E.A. McCliment, H.W. Ducklow and S.M. Huse. 2009. A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes. PloS ONE 4(7): e6372.   DOI
19 Carroll, E.L., R. Gallego, M.A. Sewell, J. Zeldis, L. Ranjard, H.A. Ross and R. Constantine. 2019. Multi-locus DNA metabarcoding of zooplankton communities and scat reveal trophic interactions of a generalist predator. Scientific Reports 9(1): 1-14.   DOI
20 Clarke, L.J., J.M. Beard, K.M. Swadling and B.E. Deagle. 2017. Effect of marker choice and thermal cycling protocol on zooplankton DNA metabarcoding studies. Ecology and Evolution 7(3): 873-883.   DOI
21 Einsle, U. 1993. Crustacea, Copepoda, Calanoida und Cyclopoida. Susswasswefauna von Mitteleuropa, vol. 8, part 4-1. Gustav Fisher Verlag, Stuttugart.
22 Guo, K., W. Zhao, Z. Jiang and S. Dong. 2017. A study of organic carbon, nitrogen and phosphorus budget in jellyfish-shellfish-fish-prawn polyculture ponds. Aquaculture Research 48(1): 68-76.   DOI
23 McCann, K.S. 2000. The diversity-stability debate. Nature 405(6783): 228-233.   DOI
24 Cloern, J.E. 1982. Does the benthos control phytoplankton biomass in south San Francisco Bay. Marine Ecology Progress Series. Oldendorf 9(2): 191-202.   DOI
25 Davenport, J., R.J. Smith and M. Packer. 2000. Mussels Mytilus edulis: significant consumers and destroyers of mesozooplankton. Marine Ecology Progress Series 198: 131-137.   DOI
26 Kim, H.J., T.K. Lee, S.W. Jung, I.K. Kwon and J.W. Yoo. 2018. Analyzing Vomit of Platalea minor(Black-faced Spoonbill) to Identify Food Components using Next-Generation Sequencing and Microscopy. Korean Journal of Environmental Biology 36(2): 165-173 (in Korean).   DOI
27 Lukwambe, B., W. Yang, Y. Zheng, R. Nicholaus, J. Zhu and Z. Zheng. 2018. Bioturbation by the razor clam(Sinonovacula constricta) on the microbial community and enzymatic activities in the sediment of an ecological aquaculture wastewater treatment system. Science of the Total Environment 643: 1098-1107.   DOI
28 Magoc, T. and S.L. Salzberg. 2011. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27(21): 2957-2963.   DOI
29 Koste, W. 1978. Rotatoria. Die Radertiere Mitteleuropes begrunder von Max Voigt, 2nd edn., Vol. 1. Textband, p. 673, Vol. 2. Tafelband, p. 234, Borntraeger, Stuttgart.
30 Maoxiao, P., L. Xiaojun, N. Donghong, Y. Bo, L. Tianyi, D. Zhiguo and L. Jiale. 2019. Survival, growth and physiology of marine bivalve (Sinonovacula constricta) in long-term low-salt culture. Scientific Reports 9(1): 1-9.   DOI
31 Na, Y.K., H. Jo, J.W. Park, K.H. Chang and I.S. Kwak. 2020. The Gut Content Analysis of Polypedilum scalaenum in the Large-scale Weirs of 4 Major River Ecosystems. Korean Journal of Ecology and Environment 53(1): 55-62 (in Korean).   DOI
32 Smirnov, N.N. and B.V. Timms. 1983. A revision of the Australian Cladocera (Crustacean). Records of the Australian Museum Supplement 1: 1-132.   DOI
33 Niu, D., L. Wang, F. Sun, Z. Liu and J. Li. 2013. Development of molecular resources for an intertidal clam, Sinonovacula constricta, using 454 transcriptome sequencing. PLoS ONE 8(7): e67456.   DOI
34 Niu, D., L. Wang, L.Z. Bai, S. Xie, H. Zhao and J. Li. 2015. Identification and expression characterization of the myostatin (MSTN) gene and association analysis with growth traits in the razor clam Sinonovacula constricta. Gene 555(2): 297-304.   DOI
35 Schloss, P.D., S.L. Westcott, T. Ryabin, J.R. Hall, M. Hartmann, E.B. Hollister, R.A. Lesniewski, B.B. Oakley, D.H. Parks, C.J. Robinson, J.W. Sahl, B. Stres, G.G. Thallinger, D.J. Van Horn and C.F. Weber. 2009. Introducing mothur: opensource, platform-independent, community-supported software for describing and comparing microbial communities. Applied and Environmental Microbiology 75(23): 7537-7541   DOI
36 Thomsen, P.F. and E. Willerslev. 2015. Environmental DNA - An emerging tool in conservation for monitoring past and present biodiversity. Biological Conservation 183: 4-18.   DOI
37 Round, F.E., R.M. Crawford and D.G. Mann. 1990. The Diatoms: Biology & morphology of the genera. Cambridge [England: Cambridge University Press.
38 Suh, J., T. Kim, S. Shin, H.Y. Kahng, S. Ahn, J.S. Jung and N.I. Won. 2017. Geochemical Characteristics and Benthos Distribution in the Three Shellfish Farms in Suncheon Bay, Korea. Journal of Environmental Science International 26(6): 691-710 (in Korean).   DOI