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http://dx.doi.org/10.11614/KSL.2021.54.3.151

Application and Utilization of Environmental DNA Technology for Biodiversity in Water Ecosystems  

Kwak, Ihn-Sil (Department of Ocean Integrated Science, Chonnam National University)
Park, Young-Seuk (Department of Biology, Kyung Hee University)
Chang, Kwang-Hyeon (Department of Environmental Science and Engineering, Kyung Hee University)
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Abstract
The application of environmental DNA in the domestic ecosystem is also accelerating, but the processing and analysis of the produced data is limited, and doubts are raised about the reliability of the analyzed and produced biological taxa identification data, and the sample medium (target sample, water, air, sediment, Gastric contents, feces, etc.) and quantification and improvement of analysis methods are also needed. Therefore, in order to secure the reliability and accuracy of biodiversity research using the environmental DNA of the domestic ecosystem, it is a process of actively using the database accumulated through ecological taxonomy and undergoing verification procedures, and experts verifying the resolution of the data increased by gene sequence analysis. This is absolutely necessary. Environmental DNA research cannot be solved only by applying molecular biology technology, and interdisciplinary research cooperation such as ecology-taxa identification-genetics-informatics is important to secure the reliability of the produced data, and researchers dealing with various media can approach it together. It is an area in desperate need of an information sharing platform that can do this, and the speed of development will proceed rapidly, and the accumulated data is expected to grow as big data within a few years.
Keywords
environmental DNA; DNA barcoding; metabarcoding;
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1 Taberlet, P., E. Coissac, M. Hajibabaei and L.H. Rieseberg. 2012. Environmental DNA. Molecular Ecology 21: 1789-1793.   DOI
2 Taberlet, P., A. Bonin, L. Zinger and E. Coissac. 2018. Environmental DNA for biobiversity research and monitoring. Oxford University Press.
3 Tringe, S.G., C. Von Mering, A. Kobayashi, A.A. Salamov, K. Chen, H.W. Chang and E.M. Rubin. 2005. Comparative metagenomics of microbial communities. Science 308: 554-557.   DOI
4 Valentini, A., C. Miquel and P. Taberlet. 2010. DNA barcoding for honey biodiversity. Diversity 2: 610-617.   DOI
5 Willerslev, E., A.J. Hansen, J. Binladen, T.B. Brand, M.T.P. Gilbert, B. Shapiro and A. Cooper. 2003. Diverse plant and animal genetic records from Holocene and Pleistocene sediments. Science 300: 791-795.   DOI
6 Selinger, D.W., R.M. Saxena, K.J. Cheung, G.M. Church and C. Rosenow. 2003. Global RNA half-life analysis in Escherichia coli reveals positional patterns of transcript degradation. Genome Research 13: 216-223.   DOI
7 Deagle, B.E., A. Chiaradia, J. McInnes and S.N. Jarman. 2010. Pyrosequencing faecal DNA to determine diet of little penguins: is what goes in what comes out? Conservation Genetics 11: 2039-2048.   DOI
8 Dejean, T., A. Valentini, C. Miquel, P. Taberlet, E. Bellemain and C. Miaud. 2012. Improved detection of and alien invasive species through environmental DNA barcoding: the example of the American bullfrog Lithobates catesbeianus. Journal of Applied Ecology 49: 953-959.   DOI
9 Levy-Booth, D.J., R.G. Campbell, R.H. Gulden, M.M. Hart, J.R. Powell, J.N. Klironomos and K.E. Dunfield. 2007. Cycling of extracellular DNA in the soil environment. Soil Biology & Biochemistry 39: 2977-2991.   DOI
10 Logan, J.M.J., K.J. Edwards and N.A. Saunders. 2009. Real-time PCR: current technology and applications. Horizon Scientific Press Co., Norwich, UK.
11 Pompanon, F., B.E. Deagle, W.O.C. Symondson, D.S. Brown, S.N. Jarman and P. Taberlet. 2012. Who is eating what: diet assessment using next generation sequencing. Molecular Ecology 21: 1931-1950.   DOI
12 Saiki, R.K., D.H. Gelfand, S. Stoffel, S.J. Scharf, R. Higuchi, T.G. Horn and H.A. Erlich. 1998. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487-491.   DOI
13 Deininger, P.L. 1983. Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis. Analytical Biochemistry 129: 216-223.   DOI
14 Thomsen, P.F., J. Kielgast, L.L. Iversen, P.R. Moller, M. Rasmussen and E. Willerslev. 2012. Detection of a diverse marine fish fauna using environmental DNA from seawater samplers. Plos One 7: e41732.   DOI
15 Ogram, A., G.S. Sayler and T. Barkay. 1987. The extraction and purification of microbial DNA from sediments. Journal of Microbiological Methods 7: 57-66.   DOI
16 Riaz, T., W. Shehzad, A. Viari, F. Pompanon, P. Taberlet and E. Coissac. 2011. ecoPrimers: inference of new DNA barcode markers from whole genome sequence analysis. Nucleic Acids Research 39: e145.   DOI
17 Simon, C. and R. Daniel. 2011. Metagenomic analyses: past and future trends. Applied and Environmental Microbiology 77: 1153-1161.   DOI
18 White, T.J., N. Arnheim and H.A. Erlich. 1989. The polymerase chain reaction. Trends in Genetics 5: 185-189.   DOI
19 Giovannoni, S.J., T.B. Britschgi, C.L. Moyer and K.G. Field. 1990. Genetic diversity in Sargasso Sea bacterioplankton. Nature 345: 60-63.   DOI
20 Ficetola, G.F., C. Miaud, F. Pompanon and P. Taberlet. 2008. Species detection using environmental DNA from water samples. Biology Letters 4: 423-425.   DOI
21 Pompanon, F., E. Coissac and P. Taberlet. 2011. Metabarcoding a new way to analyze biodiversity. Biofutur 319: 30-32.