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http://dx.doi.org/10.11614/KSL.2016.49.4.393

Distribution of Toxic and Non-toxic Microcystis in Korean Water Supply  

Lee, Kyung-Lak (Watershed Ecology Research Team, National Institute of Environmental Research)
Shin, Yuna (Watershed Ecology Research Team, National Institute of Environmental Research)
Lee, Jaean (Watershed Ecology Research Team, National Institute of Environmental Research)
Lee, Jae-Kwan (Water Environment Research Department, National Institute of Environmental Research)
Kim, Han Soon (Department of Biology, Kyungpook National University)
Publication Information
Korean Journal of Ecology and Environment / v.49, no.4, 2016 , pp. 393-399 More about this Journal
Abstract
This study was conducted to investigate whether the presence of mcy gene and microcystin production are related to morphological characteristics of Korean Microcystis species. We isolated 6 different species of Microcystis (M. aeruginosa, M. ichthyoblabe, M. flos-aquae, M. novacekii, M. viridis and M. wesenbergii) from drinking water supply dams (Yeongchun, Ankei, Gachang), and used microscopic method for morphological identification, molecular method for amplifying a partial region of mcyB gene and ELISA method for microcystin analysis. In the present study, 80% of M. aeruginosa strains contained mcy gene, followed by 45% (10 strains) of M. icthyoblabe, 33% (1 strain) of M. wesenbergii, and 11% (4 strains) of M. flos-aquae. Each percentage of mcy gene in Microcystis morphospecies was similar to that of microcystin production in Microcystis morophospecies. In conclusion, the present study shows that molecular method using mcy gene primers can be used as an indirect indicator for the monitoring of toxic cyanobacteria (Microcystis).
Keywords
Microcystis; ELISA; mcy gene; microcystin;
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1 Vezie, C., L. Brient, K. Sivonen, G. Bertru, J.-C. Lefeuvre and M. Salkinoja-Salonen. 1998. Variation of microcystin content of cyanobacterial blooms and isolated strains in Lake Grand-Lieu (France). Microbial Ecology 35: 126-135.   DOI
2 Via-Ordorika, L., J. Fastner, R. Kurmayer, M. Hisbergues, E. Dittmann, J. Komarek, M. Erhard and I. Chorus. 2004. Distribution of microcystin-producing and non-microcystin-producing Microcystis sp. in European freshwater bodies: detection of microcystins and microcystin genes in individual colonies. Systematic and Applied Microbiology 27: 592-602.   DOI
3 Watanabe, M.F. 1996. Production of microcystins, p. 13-34. In: Toxic Microcystis (Watanabe, M.F., K.-I. Harada, W.W. Carmichael and H. Fujiki eds.). CRC Press, Boca Raton.
4 Pavagadhi, S. and R. Balasubramanian. 2013. Toxicological evaluation of microcystins in aquatic fish species: Current knowledge and future directions. Aquatic Toxicology 142-143: 1-16.   DOI
5 Rantala, A., D.P. Fewer, M. Hisbergues, L. Rouhiainen, J. Vaitomaa, T. Borner and K. Sivonen. 2004. Phylogenetic evidence for the early evolution of microcystin synthesis. 568-573.
6 Rohrlack, T., M. Henning and J.-G. Kohl. 2001. Isolation and characterization of colony-forming Microcystis aeruginosa strains, p. 152-158. In: Cyanotoxins-Occurrence, Causes, Consequences (Chorus, I. ed.). Springer, New York.
7 Romero-Oliva, C.S., V. Contardo-Jara, T. Block and S. Pflugmacher. 2014. Accumulation of microcystin congeners in different aquatic plants and crops - A case study from lake Amatitlan, Guatemala. Ecotoxicology and Environmental Safety 102: 121-128.   DOI
8 Sabour, B., M. Loudiki, B. Oudra, V. Vasconcelos, R. Martins, S. Oubraim and B. Fawzi. 2002. Toxicology of a Microcystis ichthyoblabe waterbloom from Lake Oued Mellah (Morocco). Environtal Toxicology 17: 24-31.   DOI
9 Sivonen, K. and J. Jones, 1999. Cyanobacterial toxins, p. 41-111. In: Toxic cyanobacteria in water. a guide to their public health consequences, monitoring, and management (Chorus, I. and J. Bartram eds.). E and FN Spon (on behalf of WHO), London.
10 Runnegar, M., N. Berndt and N. Kaplowitz. 1995. Microcystin uptake and inhibition of protein phosphatases: effects of chemoprotectants and self-inhibition in relation to known hepatic transporters. Toxicology and Applied Pharmacology 134: 264-272.   DOI
11 Saker, M.L., J. Fastner, G. Dittmann, G. Christiansen and V.M. Vasconcelos. 2005. Variation between strains of the cyanobacterium Microcystis aeruginosa isolated from a Portuguese river. Journal of Applied Microbiology 99: 749-757.   DOI
12 Dittmann, E., B.A. Neilan and T. Borner. 1999. Peptide synthetase genes occur in various species of cyanobacteria. p. 615-621. In: The phototroph prokaryotes (Peschek, G.A., W. Loeffelhardt and G. Schmetterer, eds.). Kluwer Academic/Plenum Press, New York.
13 Baker, J.A., B. Entsch, B.A. Neilan and D.B. McKay. 2002. Monitoring changing toxigenicity of a cyanobacterial bloom by molecular methods. Applied and Environmental Microbiology 68: 6070-6076.   DOI
14 Bartram, J., M. Burch, I.R. Falconer, G. Jones and T. Kuiper-Goodman. 1999. Situation assessment, planning and management, p. 179-209. In: Toxic cyanobacteria in water: a guide to their public health consequences, monitoring, and management (Chorus, I. and J. Bartram, eds.). E and FN Spon (on behalf of WHO), London.
15 Bittencourt-Oliveira, M. 2003. Detection of potential microcystin-producing cyanobacteria in Brazilian reservoirs with a mcyB molecular marker. Harmful Algae 2: 51-60.   DOI
16 Ibelings, B.W., K. Bruning, J. de Jonge, K. Wolfstein, L.M.D. Pires, J. Postma and T. Burger. 2005. Distribution of microcystin in a lake foodweb: no evidence for biomagnification. Microbial Ecology 49(4): 487-500.   DOI
17 Kurmayer, R., E. Dittmann, J. Fastner and I. Chorus. 2002. Diversity of microcystin genes within a population of the toxic cyanobacterium Microcystis spp. in Lake Wannsee (Berlin, Germany). Microbial Ecology 43: 107-118.   DOI
18 Komarek, J. 1991. A review of water bloom forming Microcystis species, with regard to populations from Japan. Archiv fur Hydrobiologie 64: 115-127 (Suppl. 92, Algological studies).
19 Komarek, J. and K. Anagnostidis. 1999. Cyanoprokaryota 1. Teil: Chroococcales, p. 1-548. In: Susswasserflora von Mitteleuropa (Ettl, H., G. Gartner, H. Heynig and D. Mollenhauer eds.). Spektrum Akademischer Verlag, Heidelberg.
20 Komarek, J. and J. Komarkova. 2002. Review of the European Microcystis morphospecies (Cyanoprokaryotes) from nature. Czech Phycology, Olomouc 2: 1-24.
21 Kurmayer, R., G. Christiansen, J. Fastner and T. Borner. 2004. Abundance of active and inactive microcystin genotypes in population of the toxic cyanobacterium Planktothrix spp. Environmental Microbiology 6: 831-841.   DOI
22 Lee, K.L., W.H. Jheong, J.M. Kim, Y.S. Kim, H.J. Choi and H.S. Kim. Seasonal variations of cyanobacterial toxins (microcystins) in Yeongchun Reservoir. Korean Journal of Limnological Society 41(2): 264-274.
23 Tillett, D., D.L. Parker and B.A. Neilan. 2001. Detection of toxigenicity by a probe for the microcystin synthetase A gene (mcyA) of the cyanobacterial genus Microcystis: comparison of toxicities with 16S rRNA and phycocyanin operon (phycocyanin intergenic spacer) phylogenies. Applied and Environmental Microbiology 67: 2810-2818.
24 Tillett, D., E. Dittmann, M. Erhard, H. von Dohren, T. Borner and B.A. Neilan. 2000. Structural organization of microcystin biosynthesis in Microcystis aeruginosa PCC7806: an integrated peptide-polyketide synthetase system. The Journal of Chemical Biology 7: 753-764.   DOI
25 Ozawa, K., H. Fujioka, M. Muranaka, A. Yokoyama, Y. Katagami, T. Homma, K. Ishikawa, S. Tsujimura, M. Kumagai, M.F. Watanabe and H.-D. Park. 2005. Spatial distribution and temporal variation of Microcystis species composition and microcystin concentration in Lake Biwa. Environtal Toxicology 20: 270-276.   DOI
26 MacKintosh, C., K.A. Beattie, S. Klumpp, P. Cohen and G.A. Codd. 1990. Cyanobacterial microcystin-LR is a potent and specific inhibitor of protein phosphatases 1 and 2A from both mammals and higher plants. FEBS Letters 264: 187-192.   DOI
27 Neilan, B.A., E. Dittmann, L. Rouhiainen, A. Bass, V. Schaub, K. Sivonen and T. Boner. 1999. Non-ribosomal peptide synthesis and toxigenicity of cyanobacteria. Journal of Bacteriology 181: 4089-4097.
28 Nishizawa, T., M. Asayama, K. Fujii, K. Harada and M. Shirai. 1999. Genetic analysis of the peptide synthetase genes for a cyclic heptapeptide microcystin in Microcystis spp. The Journal of Biochemistry 126: 520-529.   DOI
29 Otsuka, S., S. Suda, R. Li, S. Matsumoto. and M.M. Watanabe. 2000. Morphological vaiability of colonies of Microcystis morphospecies in culture. The Journal of General and Applied Microbiology 46: 39-50.   DOI
30 Otsuka, S., S. Suda, S. Shibata, H. Oyaizu, S. Matsumoto and M.M. Watanabe. 2001. A proposal for the unification of five species of the cyanobacterial genus Microcystis Kutzing ex Lemmermann 1907 under the Rules of the Bacteriological Code. International Journal of Systematic and Evolutionary Microbiology 51: 873-879.   DOI