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http://dx.doi.org/10.11614/KSL.2014.47.4.342

Identification and Analysis of Geosmin Production Potential of Anabaena stain Isolated from North Han River using Genetic Methods  

Kim, Keon Hee (Department of Environmental Science, Konkuk University)
Lim, Byung-Jin (Han River Environment Research Center, National Institute of Environmental Research)
You, Kyoung-A (Han River Environment Research Center, National Institute of Environmental Research)
Park, Myung-Hwan (Department of Environmental Science, Konkuk University)
Park, Jung Hwan (Department of Environmental Science, Konkuk University)
Kim, Baik-Ho (Department of Life Science, Hanyang University)
Hwang, Soon-Jin (Department of Environmental Science, Konkuk University)
Publication Information
Korean Journal of Ecology and Environment / v.47, no.4, 2014 , pp. 342-349 More about this Journal
Abstract
This study was conducted to identify the bloom-forming Anabaena strains with two phenotypes (straight-type and coil-type) isolated from North Han River (Sambong-ri Joam-myun) using 16S rDNA sequence. The odor material producing potential was also examined using the geosmin-synthesizing gene cluster. Despite of striking morphological difference of the two stains, resembling A. circinalis and A. crassa species, the phylogenetic analysis using 16S rDNA identified the both strains as a single species of A. circinalis with high genetic similarity (98%~100%). Also, two Anabaena strains showed to possess the geosmin-synthesizing gene cluster, indicating that they are capable of producing the odor substance. This study is the first report that provides the direct evidence of geosmin production in the gene level by A. circinalis in Korea, and provides important basic information to identify the source alga of geosmin increase and its management in North Han River.
Keywords
Anabaena circinalis; phenotypes; 16S rDNA; geosmin production potential; North Han River;
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