Browse > Article
http://dx.doi.org/10.11614/KSL.2013.46.2.301

Molecular Identification of the Bloom-forming Cyanobacterium Anabaena from North Han River System in Summer 2012  

Li, Zhun (Department of Life Science, Hanyang University)
Han, Myung-Soo (Department of Life Science, Hanyang University)
Hwang, Su-Ok (K-waters, Korea Water Resources Corporation)
Byeon, Myeong-Seop (Han River Environment Research Center, National Institute of Environmental Research)
Hwang, Soon-Jin (Department of Environmental Science, Konkuk University)
Kim, Baik-Ho (Department of Life Science, Hanyang University)
Publication Information
Korean Journal of Ecology and Environment / v.46, no.2, 2013 , pp. 301-309 More about this Journal
Abstract
Between May and August 2012, a massive cyanobacterial bloom with Anabaena has been occurred throughout the North Han River. Sampling was conducted at one station on each lake, L. Uham, L. Cheongpyung, and L. Paldang, where occurred a dense bloom, in 13 July. According to the microscopic examination, the blooms was dominated by one specific filamentous cyanobacterium Anabaena and other phytoplankton. Morphologically, previous literature proven that this Anabaena species is A. crassa (Lemmermann) Komark.-Legn. & Cronberg. However, identification of species in a mixed population is complicated due to limited morphological differences. Therefore, with live sample including trichome, akinete and heterocyst, the sequences of 16S rRNA gene of Anabaena isolates were cloned and analyzed, and three 16S rRNA gene sequences of 1188~1520 bp in length were obtained. It was shown from the homologous analysis results that the obtained 16S rRNA sequences were highly homologous to the relevant sequences of A. crassa in GenBank. The 16S rRNA sequences of 63 species were retrieved from GenBank, and the phylogenetic tree was constructed by using these sequences.
Keywords
Cyanobacterium; Anabaena crassa; Han River; Harmful Algal Bloom; 16S rRNA;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Hall, T.A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/ 98/NT. Nucleic Acids Symposium Series 41: 95-98.
2 Becker, V., P. Ihara, J.S. Yunes and V.L.M. Huszar. 2010. Occurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil. Journal of Applied Phycology 22: 235-241.   DOI
3 Cho, K.S., B.C. Kim, W.M. Heo and S.J. Cho. 1989. The succession of phytoplankton in Lake Soyang. Korean Journal of Limnological Society 10: 73-82.
4 Cook, W.O., V.R. Beasley, R.A. Lovell, A.M. Dahlem, S.B. Hooser, N.A. Mahmood and W.W. Carmichael. 1989. Consistent inhibition of peripheral cholinesterases by neurotoxins from the freshwater cyanobacterium Anabaena flos-aquae: studies of ducks, swine, mice and a steer. Environmental Toxicology and Chemistry 8: 915-922.   DOI
5 Frizzo, E.E., V. Becker, F.B. Spiandorello, R. Girardi and J.S. Yunes. 2004. Implantac ao do monitoramento fitoplanctonico, com enfase em cianobacterias e cianotoxinas, nos mananciais de abastecimento do municipio de Caxias do Sul, RS. In Anais VIII Exposicao de experiencias municipais em saneamento/34a Assembleia Nacional da ASSEMAE. ASSEMAE, Caxias do Sul, 421-424.
6 Guindon, S. and O. Gascuel. 2003. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology 52: 696-704.   DOI
7 Hayes, K.P. and M.D. Burch. 1989. Odorous compounds associated with algal blooms in South Australian waters. Water Research 23: 115-121.   DOI
8 Henriksen, P., W.W. Carmichael, J. An and O. Mostrup. 1997. Detection of an anatoxin-a(s)-like anticholinesterase in natural blooms and cultures of cyanobacteria/ blue-green algae from Danish lakes and in the stomach contents of poisoned birds. Toxicon 35: 901-913.   DOI
9 Izaguirre, G., C.J. Hwang, S.W. Krasner and M.J. McGuire. 1982. Geosmin and 2-methylisoborneol from cyanobacteria in three water supply systems. Applied and Environmental Microbiology 43: 708-714.
10 Jung, S.W., J.H. Lee and H.K. Heo. 2004. Environmental studies in the lower part of the Han River VI. The statistical analysis of eutrophication factor. Korean Journal of Limnological Society 37: 78-86.
11 Lee, D.S., S.Q. Suh, S.K. Hwang, T.K. Kwon, T.H. Kim, W.C. Shin and S.D. Hong. 1996. Nucleotide sequence of 16S rRNA gene from Streptomyces melanosporofaciens 7489. Journal of Microbiology and Biotechnology 6: 364-365.
12 Kim, B.H., J.Y. Choi, M.S. Han and S.J. Hwang. 2004. Influences of nutrient deficiency on the phytoplankton in Paltang reservoir, Korea. Korean Journal of Limnological Society 37: 47-56.
13 Komarek, J. and E. Zapomelova. 2007. Planktic morphospecies of the cyanobacterial genus Anabaena=subg. Dolichospermum - 1. part: coiled types. Fottea 7: 1-31.
14 Kwon, O.Y., S.W. Chung and J.W. Lee. 2006. Environmental studies in the lower part of the Han River VIII. Physicochemical factors contributing to variation of phytoplankton communities. Korean Journal of Limnological Society 39: 340-351.
15 Lee, E.J. and I.H. Choi. 2012. Variation of the phytoplankton community and analysis of odor compounds in Han River. Co-conferences of Korean Society of water Environment and Korean Society of Drinking Water. March 21-22. KINTEX.
16 Lee, E.J., B.C. Kim and K.S. Cho. 1998. Patterns of phytoplankton community structure at inlet site (Sanggul-Ri) in Lake Soyang from 1984 to 1997. Korean Journal of Limnological Society 31: 119-128.
17 Lim, J.R., S.S. Kim, K.H. Lee, S.Y. Lee, H.J. Cheon and S.Y. Chung. 2006. The relation of occurrence of algae and taste and odeor in water treatment plant around the Han River water supply system. Conferences of Korean Society of Water Environment Engineering. April 27-29. pp. 248-251. KINTEX.
18 Mahmood, N.A., W.W. Carmichael and D. Pfahler. 1988. Anticholinesterase poisonings in dogs from a cyanobacterial (blue-green algae) bloom dominated by Anabaena flosaquae. American Journal of Veterinary Research 49: 500-503.
19 Saadoun, I., K.K. Schrader and W.T. Blevins. 2001. Identification of geosmin as a volatile metabolite of Anabaena sp. Journal of Basic Microbiology 41: 51-55.   DOI
20 Molica, R.J.R., E.J.A. Oliveira, P.V.V.C. Carvalho, A.N.S.F. Costa, M.C.C. Cunha and G.L. Melo. 2005. Occurrence of saxitoxins and an anatoxin-a(s)-like anticholinesterase in a Brazilian drinking water supply. Harmful Algae 4: 743-753.   DOI
21 Monserrat, J.M., J.S. Yunes and A. Bianchini. 2001. Effects of Anabaena spiroides (Cyanobacteria) aqueos extracts on the Aceltycholinesterase activity of aquatic species. Environmental Toxicology and Chemistry 20: 1228-1235.   DOI
22 Nubel, U., F. Garcia-Pichel and G. Muyzer. 1997. PCR primers to amplify 16S rRNA genes from cyanobacteria. Applied and Environmental Microbiology 63: 3327-3332.
23 Seo, M.Y., B.H. Kim and K.S. Bae. 2007. Fluctuation of environmental factors and dynamics of phytoplankton communities in lower part of the Han River. Korean Journal of Limnological Society 40: 395-402.
24 Swofford, D.L. 2002. PAUP (Phylogenetic Analysis Using Parsimony) and other Methods, ver. 4b10. Sinauer Associates, Sunderland, Massachusetts.
25 Thompson, J.D., D.G. Higgins and T.J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Symposium Series 22: 4673-4680.
26 Tuji, A. and Y. Niiyama. 2010. Phylogenetic study by the morphological and molecular analyses of Japanese planktonic Anabaena Species. Bulletin of the National Science Museum Series B 36: 71-80.
27 Willame, R., C. Boutte, S. Grubisic, A. Wilmotte, J. Komarek and L. Hoffmann. 2006. Morphological and molecular characterization of planktonic cyanobacteria from Belgium and Luxembourg. Journal of Phycology 42: 1312-1332.   DOI
28 Urbach, E., D. Robertsin and S. Chisholm. 1992. Multiple evolutionary origins of prochlorophytes within the cyanobacterial radiation. Nature 355: 267-270.   DOI
29 Wang, S.H., A.R. Dzialowski, J.O. Meyer, F. de Noyelles, N.C. Lim, W.W. Spotts and D.G. Huggins. 2005. Relationships between cyanobacterial production and the physical and chemical properties of a Midwestern Reservoir, USA. Hydrobiologia 541: 29-43.   DOI
30 Werner, V.R. and H.D. Laughinghouse. 2009. Bloom-forming and other planktonic Anabaena (Cyanobacteria) morphospecies with twisted trichomes from Rio Grande do Sul State, Brazil. Nova Hdewigia 89: 17-47.   DOI
31 You, K.A., M.S. Byeon, S.J. Youn, S.J. Hwang and D.H. Rhew. 2013. Growth Characteristics of Blue-green Algae (Anabaena spiroides) Causing Tastes and Odors in the North-Han River, Korea. Korean Journal of Ecology and Environment 46: 135-144.   DOI
32 Yunes, J.S., N.T. Cunha, L.A.O. Proenca, L.P. Barros and J.M. Monserrat. 2003. Cyanobacterial neurotoxins from Southern Brazilian freshwaters. Comments on Toxicology 9: 103-115.   DOI
33 Yunes, J.S., A. Matthiensen, C. Carneiro, F. Oroski, V. Becker and M.C. Carvalho. 2005. Floracoes de Cianobacterias Toxicas: Maos a obra ao problema. In: Marinho M, Roland F, Cesar D (eds) Licoes de Limnologia. RiMa, Sao Carlos, 299-323.
34 Zapomelova, E., D. Hisem, K. Rehakova, P. Hrouzek, J. Jezberova, J. Komarkova, J. Korelusova and P. Znachor. 2008. Experimental comparison of phenotypical plasticity and growth demands of two strains from the Anabaena circinalis/A. crassa complex (cyanobacteria). Journal of Plankton Research 30: 1257-1269.   DOI
35 Zapomelova, E., E. Zapomelova, O. Skacelova, P. Pumann, R. Kopp and E. Janecek. 2012. Biogeographically interesting planktonic Nostocales (Cyanobacteria) in the Czech Republic and their polyphasic evaluation resulting in taxonomic revisions of Anabaena bergii Ostenfeld 1908 (Chrysosporum gen. nov.) and A. tenericaulis Nygaard 1949 (Dolichospermum tenericaule comb. nova). Hydrobiologia 698: 353-365.   DOI