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Changes of Carassius auratus Tissues by Zn Accumulation  

Shin, Myung-Ja (Department of Biological Science, Andong National University)
Lee, Jong-Eun (Department of Biological Science, Andong National University)
Seo, Eul-Won (Department of Biological Science, Andong National University)
Publication Information
Korean Journal of Ecology and Environment / v.44, no.1, 2011 , pp. 85-94 More about this Journal
Abstract
Present study aimed to investigate the effect of zinc accumulation on the tissues of Carassius auratus with various rearing condition. Heavy metal, Zn, was accumulated in the gill, bone and muscle increased for exposure period of 40 days. Moreover, the accumulated concentration of Zn in the gill showed relatively higher than that in other tissues. Also, activities of antioxidant enzymes in the gill, kidney, liver and muscle tissues after exposure to Zn were increased in the manner of exposure period. Antioxidant enzyme activities were shown higher in the gill and muscle than those in the kidney and liver tissues. The gill of C. auratus showed abnormal shapes in its secondary lamellae by long time exposure condition and determined the increased numbers of mucous cells in gill exposed to Zn. In addition, the edema, the exfoliation of epithelial cell was shown in the gill and membrane damages were observed in mitochondria and nucleus. In the kidney, we observed not only the atrophied glomerulus, but the expansion of empty space in Bowman's capsule. Based on the above results, it is considered that the exposure to the high level of Zn for long period possibly involves in the decrease of respiratory and excretory rates, giving rise to secondary lesion of tissues.
Keywords
Carassius auratus; Zn; antioxidant enzyme; tissues;
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1 Shin, M.J., Y.M. Park, J.E. Lee and E.W. Seo. 2010a. Heavy metal contents in tissues of fishes in Andong and Imha reservoirs. Korean Journal of Life Science 20: 1378-1384.   DOI
2 Shin, M.J., O.C. Kwon, J.E. Lee and E.W. Seo. 2010b. Effects of cadmium exposure on tissues of Carassius auratus. Korean Journal of Life Science 20: 1490-1497.   DOI
3 Shin, M.Y., D.L. Choi, J.K. Chung and S.K. Chun. 1993. Histopathological changes in fish gills by potassium permanganate and influence of water quality. Journal of Fish Pathology 6: 21-55.
4 Soh, C.T., I.S. Yoo, H. Park, S.H. Kim, J.J. Kim and D.Y. Min. 1993. Experimental study on the effect of cadmium containing shellfish by the long term intake. Korean Journal of Malacology 9: 85-93.
5 Sorensen, E.M. 1991. Cadmium, p. 175-234. In: Metal Poisoning in Fish., CRC press, Boston.
6 Watzin, M.C. and P.R. Roscigno. 1997. The effects of zinc contamination on the recruitment and early survival of benethic invertebrates in an estuary. Marine Pollution Bulletin 34: 443-455.   DOI   ScienceOn
7 Wendel, A. and S. Feuerstin. 1981. Drug-induced lipid peroxidation in mice-l. Modulation by monoxygenase activity, glutathione and selsnium status. Biochemical Pharmacology 30: 2513-2520.   DOI   ScienceOn
8 Wicklund, A., L. Norrgren and P. Runn. 1990. The influence of cadmium and zinc on cadmium turnover in the zebrafish, Brachydanio rerio. Archives of Environmental Contamination and Toxicology 3: 348-353.
9 You, K.H., C.K. Choi, R.S. Choe and Y.K. Deung. 1978. Ultrastructural studies on cadmium poisoning in the liver, kidney and gills of Carassius carassius. Korean Journal Electron Microscopy 8: 33-48.
10 Zelikoff, J.T., D. Bowser, K.S. Squibb and K. Frenkel. 1995. Immunotoxicity of low level cadmium exposure in fish, an alternative animal model for immunotoxicological studies. Journal of Toxicology and Environmental Health 45: 235-248.   DOI   ScienceOn
11 Zikic, R.V., A.S. Stajn, S.Z. Pavlovic, B.I. Ognjanovic and Z.S. Saicic. 2001. Activities of superoxide dismutase and catalase in erythrocytes and plasma transaminases of goldfish (Carassius auratus gibelio Bloch) exposed to cadmium. Physiological Research 50: 105-111.
12 Kimura, L., M. Yamashita, N. Kinae, H. Kumai and G. Nakamura. 1990. The croaker (Nibea mitsukurii) and the sea catfish (Plotosus anguillaris): Useful biomarkers of coastal pollution, p. 73-84. In: Biomarkers of Environmental Contamination (Publisher, L. ed.). Academic Press. Florida.
13 Lee, C., M.J. Shin, J.E. Lee and E.W. Seo. 2006. Tissues and plasma proteins of Hemiculter eigenmanni in muddy water of reservoir. Korean Journal of Environmental Biology 24: 213-220.
14 Magee, A.C. and S. Spahr. 1964. Effects of dietary supplements on young rats fed high levels of zinc. Journal of Nutrition 82: 209-216.   DOI
15 Lee, J.C., J.C. Kang, Y.K. Shin, K.H. Ma and P. Chin. 2001. Histological responses of the flounder, Paralichthys olivaceus exposed to copper. Journal of Fish Pathology 14: 81-90.
16 Lee, K.H. 2002. A study on adsorption behaviors of activated carbon powder and loess for heavy metal in aqueous solution. Journal of the Korean Society for Environmental Analysis 5: 123-130.
17 Lee, Y.C., Y.J. Chang and B.K. Lee. 1997. Osmoregulation capability of juvenile grey mullets (Mugil cephalus) with the different salinities. Journal of the Korean Fisheries Society 30: 216-224.
18 McCord, J.M. and I. Fridovich. 1969. Superoxide dismutase an enzymic function ferythrocuprotein (Hemocuprotein). Journal of Biological Chemistry 244: 6049-6055.
19 McGeer, J.C., C. Szebedinszky, D.F. McDonald and C.M. Wood. 2000. Effects of chronic sublethal exposure to waterbrne Cu, Cd or Zn in rainbow trout. 1: Iono-regulatory disturbance and metabolic costs. Aquatic Toxicology 50: 231-243.   DOI   ScienceOn
20 Park, S.H., I.S. Song and Y.C. Cho. 2000. Experimental study on the accumulation of cadmium and other metals in the fish bodies (Oryzias latipes). Korean Journal of Environmental Health Society 26: 25-31.
21 Seo, E.W., Y.J. Kim and S.C. Baek. 2008. Soil components and elution characteristics of heavy metals in sediments of Andong and Imha reservoirs. Journal of the Korean Geotechnical Society 9: 47-52.
22 Fennel, R.H. and V.M. Pardo. 1967. Experimental glomerulonephritis in rats. Laboratory Investigation 17: 483-488.
23 Flohe, L., A. Wolfgang and W.A. Gunzler. 1984. Assay of glutathione peroxidase, p. 105-114. In: Methods in enzymatic analysis (Packer, L. ed.). Academic Press, New York.
24 Forman, H.J. and I. Fridovich. 1973. Superoxide dismutase: A comparison of rate constant. Archives of Biochemistry and Biophysics 158: 396-400.   DOI   ScienceOn
25 Friberg, L. and J. Vosta. 1972. Mercury in the environment. p. 17-23. CRS Press, Cleveland.
26 Jeon, J.S., J.S. Kim and B.C. Koo. 1993. The effect of cadmium administration in seminiferous epithelium of mouse testes: electron microscopic study. Korean Journal of Electron Microscopy 23: 125-138.
27 Gabrylak, T., M. Piatrowska, W. Leyko and G. Peres. 1983. Seasonal variation in the activities of peroxide metabolism enzymes in erythrocytes of freshwater fish species. Comparative Biochemistry and Physiology 75: 383-385.
28 Huh, M.D. and H.D. Jeong. 1993. The histological structure and the pathological lesions of gill in teleosts. Journal of Fish Pathology 6: 65-70.
29 Hwang, I.D., S.K. No, S.Y. Gi, J.H. Lee and N.S. Kim. 1989. A study on the heavy metal contents in fish and sediments of the Mankyung River. Korean Journal of Environmental Health Society 15: 33-49.
30 Kim, J.S., M.J. Shin, J.E. Lee and E.W. Seo. 2009. Heavy metal contents in tissues of Carassius auratus in Andong and Imha Reservoirs. Korean Journal of Life Science 19: 1562-1567.   DOI
31 Kim, J.S., M.J. Shin, J.E. Lee and E.W. Seo. 2010. Morphological and physiological effects of lead (Pb) exposure on tissues of Carassius auratus. Korean Journal of Limnology 43: 409-417.
32 Aebi, H. 1984. Catalase in vitro, p. 121-126. In: Methods in Enzymology (Packer, L. ed.). Academic Press, New York.
33 Ahmad, S. 1995. Oxidative stress and antioxidant defenses in biology, p. 38-272. Chapman and Hall, New York.
34 Aksnes, A. and L.R. Njaa. 1981. Catalase, glutathione peroxidase and superoxide dismutase in different fish species. Comparative Biochemistry and Physiology 69B: 893-896.
35 APHA. 1985. Standard methods for the examination of water and wastewater (16th ed.). Port City Press, Maryland.
36 Bergman, B. and R. Soremark. 1968. Autoradiographic studies on the distribution of zinc-65 in mice. Journal of Nutrition 44: 6-12.
37 Black, J.J. and P.C. Bauman. 1991. Carcinogens and cancers in freshwater fishes. Environmental Health Perspectives 90: 27-33.   DOI
38 Chen, Y., X.D. Cao, Y. Lu and X.R. Wang. 2000. Effects of rare earth metal ions and their EDTA complexes on antioxidant enzymes of fish liver. Environmental Contamination and Toxicology 65: 357-365.   DOI   ScienceOn
39 Chance, B., H. Siec and A. Boveris. 1979. Hydroperoxide metabolism in mammalian organs. Physiological Reviews 59: 527-605.   DOI