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An Application of $^{13}C$ Tracer for the Determination of Primary Productivity and Fatty Acid Production Rate in Shingu Reservoir  

Lee, Yeon-Jung (Department of Environmental Marine Sciences, Hanyang University)
Kim, Min-Seob (Department of Environmental Marine Sciences, Hanyang University)
Shin, Kyung-Hoon (Department of Environmental Marine Sciences, Hanyang University)
Hwang, Soon-Jin (Department of Environmental Science, Konkuk University)
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Abstract
The in situ incubation experiment was carried out using $^{13}C$ tracer to determine primary productivity and fatty acid production rate in the shallow, eutrophic Shingu reservoir on 4th July. Particulate organic matter (POM) accounted for 76% in suspended particulate matter (SPM), and average concentration of chlorophyll-${\alpha}$ was $89{\mu}g\;L^{-1}$ in the euphotic layer. Total amount of chlorophyll-${\alpha}$ (Chl-${alpha}$), primary productivity and Chl-${alpha}$ specific productivity in euphotic layer were 112 mg Chl-${alpha}m^{-2}$, 3.53 g C $m^{-2}\;d^{-1}$, 32mg C mg Chl-${\alpha}^{-1}\;d^{-1}$, respectively. The fatty acid composition in newly produced organic matter and suspended organic matter didn't show any significant difference, demonstrating that autochthonous organic matter should be a major source of POM pool. In addition, the fatty acids of bacterial origin were increased through extracellular release of newly photosynthesized DOC, and closely coupled with bacterial assimilation. This result suggests that organic carbon should be actively cycled through the microbial loop in Shingu reservoir in summer.
Keywords
$^{13}C$ tracer method; primary productivity; fatty acid; Shingu reservoir;
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