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Genetic Diversity of an Endangered Fish, Iksookimia choii (Cypriniformes), from Korea as Assessed by Amplified Fragment Length Polymorphism  

Lee, Il-Ro (Department of Marine Biotechnology, Soonchunhyang University)
Lee, Yoon-A (Department of Marine Biotechnology, Soonchunhyang University)
Shin, Hyun-Chur (Department of Marine Biology, Soonchunhyang University)
Nam, Yoon-Kwon (Department of Aquaculture, Pukyong National University)
Kim, Woo-Jin (Biotechnology Research Institute, National Fisheries Research and Development Institute)
Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
Publication Information
Korean Journal of Ecology and Environment / v.41, no.1, 2008 , pp. 98-103 More about this Journal
Abstract
Genetic diversity and population genetic structure within or among three stream populations (Gab, Baekgok and Ji streams) of Korean endangered natural monument fish, Iksookimia choii, were assessed by amplified fragment length polymorphism (AFLP). AFLP analysis using three primer combinations generated 104 to 106 AFLP bands, and percent polymorphic bands were similar in those three populations ranging 21.5 to 24.5%. Heterozygosity and genetic diversity within or among populations were quite low for all of these populations with average values ranging from 0.067 to 0.084 and from 0.076 to 0.087, respectively. Analyses of pairwise distance and genetic similarity among three populations of I. choii also revealed the similar results with very low genetic differentiation one another. Although pairwise Fst values were very low, our data clearly indicated distinct genetic differentiation among the three populations. This is the first report concerning the genetic diversity and differentiation of this species, and provides basic genetic information that should facilitate attempts to conserve this species.
Keywords
endangered fish; Iksookimia choii; AFLP; genetic diversity; genetic differentiation;
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