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Detection of Toxigenicity of Cyanobacteria by Molecular Method  

Lee, Kyung-Lak (National Institute of Environmental Research)
Jheong, Weon-Hwa (National Institute of Environmental Research)
Kim, Jong-Min (National Institute of Environmental Research)
Kim, Han-Soon (Department of Biology, Kyungpook University)
Publication Information
Korean Journal of Ecology and Environment / v.40, no.1, 2007 , pp. 149-154 More about this Journal
Abstract
In the present study, we performed the PCR assay using TOX2P/TOX2M primer targeting a specific region within mcyB gene to identify potential microcystin-producing cyanobacteria. TOX2P/TOX2M primer set was effective in amplifying mcy gene in the field samples containing Microcystis spp. of 1,000 cells per mL. Moreover, the results from the PCR assay agreed with those of the ELISA analysis. Consequently, this study demonstrated that TOX2P/TOX2M primer set can be used as a genetic probe for the early detection of cyanobacterial toxigenicity in Korean water bodies.
Keywords
cyanobacteria; microcystin biosynthetase gene; toxigenicity; TOX2P/TOX2M primer; ELISA;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 환경부. 2006. 조류예보제 시행계획. 환경부 수질보전국, 서울
2 Dittmann, E., B.A. Neilan and T. Borner. 1999. Peptide synthetase genes occur in various species of cyanobacteria. p. 615-621. In: The phototrophic prokaryotes (Peschek, G.A, W. Loeffelhardt and G. Schmetterer, eds.). Kluwer Academic/Plenum Press, New York
3 Kaebernick, M. and B.A. Neilan. 2001. Ecological and molecular investigations of cyanotoxin production. FEMS Microbiol. Ecol. 35: 1-9   DOI
4 Neilan, B.A., D. Jacobs, T.D. Dot, L.L. Blackall, P.R. Hawkins, P.T. Cox and A.E. Goodman. 1997. rRNA sequences and evolutionary relationships among toxic and nontoxic cyanobacteria of the genus Microcystis. Int. J. Syst. Bacteriol. 47: 693-697   DOI   ScienceOn
5 Tillett, D., D.L. Parker and B.A. Neilan. 2001. Detection of toxigenicity by a probe for the microcystin synthetase A gene (mcyA) of the cyanobacterial genus Microcystis: comparison of toxicities with 168 rRNA and phycocyanin operon (phycocyanin intergenic spacer) phylogenies. Appl. Environ. Microbiol. 67: 2810-2818   DOI   ScienceOn
6 Dittmann, E. and C. Wiegand. 2006. Cyanobacterial toxins -occurrence, biosynthesis and impact on human affairs. Mol. Nutr. Food Res. 50: 7-17   DOI   ScienceOn
7 Baker, J.A., B.A. Neilan, B. Entsch and D.B. Mckay. 2001. Identification of cyanobacteria and their toxigenicity in environmental samples by rapid molecular analysis. Environ. Toxicol. 16: 472-482   DOI   ScienceOn
8 Sivonen, K. and G. Jones. 1999. Cyanobacterial toxins. p. 41-111. In: Toxic cyanobacteria in water-Chorus, I. and J. Bartram, eds.), A guide to their public health consequences, monitoring and management. E and FN Spon Press, London
9 Tillett, D., E. Dittmann, M. Erhard, H. von Dohren, T. Borner and B.A. Neilan. 2000. Structural organization of microcystin biosynthesis in Microcystis aeruginosa PCC7806: an integrated peptide-polyketide synthetase system. Chem. Biol. 7: 753-764   DOI   ScienceOn
10 Neilan, B.A., E. Dittmann, L. Rouhiainen, R.A. Bass, V. Schaub, K Sivonen and T. Borner. 1999. Nonribosornal peptide synthesis and toxigenicity of cyanobacteria. J. Bacteriol. 181: 4089-4097
11 Carmichael, W.W. 1988. Toxins offreshwater algae. p. 121-147. In: Marine Toxins and Venoms, Handbook of Natural Toxins (Tu, A.T., ed.). Marcel Dekker Press, New York
12 Carmichael, W.W. 1996. Cyanobacterial toxins. IOC Manuals and Guides 33: 163-175
13 Meissner, K., E. Dittmann and T. Borner. 1996. Toxic and non-toxic strains of the cyanobacterium Microcystis aeruginosa contain sequences homologous to peptide synthetase genes. FEMS Microbiol. Lett. 135: 295-303   DOI
14 Sivonen, K. 1996. Cyanobacterial toxins and toxin production. Phycologia 35: 12-24   DOI   ScienceOn
15 Dittmann, E. and B.A. Borner. 2005. Genetic contribution to the risk assessment of microcystin in the environment. Toxicol. Appl. Pharmacol. 203: 192-200   DOI   ScienceOn
16 Park, H.D., B.C. Kim, E.K. Kim and T. Okino. 1998. Hepatotoxic microcystins and neurotoxic anatoxin-a in cyanobacterial blooms from Korean lakes. Environ. Toxicol. Water Qual. 13: 225-234   DOI   ScienceOn
17 Falconer, I.R. and A.R. Humpage. 1996. Tumour promotion by cyanobacterial toxins. Phycologia 35: 74-79   DOI   ScienceOn
18 Via-Ordorika, L., J. Fastner, R. Kurmayer, M. Hisbergues, E. Dittmann, J. Komarek, M. Erhard and I. Chorus. 2004. Distribution of microcystin-producing and nonmicrocystin-producing Microcystis sp. in European freshwater bodies: detection of microcystins and microcystin genes in individual colonies. System. Appl. Microbiol. 27: 592-602   DOI   ScienceOn
19 Otsuka, S., S. Suda, R. Li, M. Watanabe, H. Oyaizu, S. Matsumoto and M.M. Watanabe. 1999. Phylogenetic relationship between toxic and nontoxic strains of the genus Microcystis based on 16S to 23S internal transcribed spacer sequence. FEMS Microbiol. Lett. 172: 15-21   DOI   ScienceOn
20 Nishizawa, T., M. Asayama, K. Fujii, K. Harada and M. Shirai. 1999. Genetic analysis of the peptide synthetase genes for a cyclic heptapeptide microcystin in Microcystis spp. J. Biochem. 126: 520-529   DOI
21 Neilan, B.A., D. Jacobs and A.E. Goodman. 1995. Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol. 61: 3875-3883
22 Watanabe, M.F. 1996. Toxic Microcystis. p. 35-56. In: Production of microcystin (Watanabe, M.F., K. Harada, W.W. Carmichael and H. Fujiki, eds.). CRC Press, Tokyo
23 김한순, 문주환. 2000. 공산호에서 수화발생동안 Microcystis aeruginosa 수직분포의 변동, 육수지 33: 119-127
24 Bittencourt-Oliveira, M.C. 2003. Detection of potential microcystin-producing cyanobacteria in Brazilian reservoirs with a mcyB molecular marker. Harmful Algae 2: 51-60   DOI   ScienceOn
25 Codd, G.A, C.J. Ward, K.A. Beattie and S.G. Bell. 1999. Widening perceptions of the occurrence and significance of cyanobacterial toxins. p. 623-632. In: The phototrophic prokaryotes (Peschek, G.A, W. Loeffelhardt and G. Schmetterer, eds.), Kluwer Academic Press, New York
26 Kemp, A and J. John. 2006. Microcystins associated with Microcystis dominated blooms in the Southwest wetlands, Western Australia. Environ. Toxicol. 21: 125-130   DOI   ScienceOn
27 김종민, 한명수, 정원화, 박준대. 2005. 팔당호에서 식물플랑크톤 군집 동태와 수질과의 상관성, 육수지 38: 217-224   과학기술학회마을
28 Carmichael, W.W. 1992. Cyanobacterial secondary metabolites-the cyanotoxins. J. Appl. Bacteriol. 72: 445-459   DOI
29 Falconer, I.R., AM. Beresford and M.T.C. Runnegar. 1983. Evidence of liver damage by toxin from a bloom of the blue-green alga Microcystis aeruginosa. Med. J. Aust. 1: 511-514
30 Pan, H., L. Song, Y. Liu and T. Borner. 2002. Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples. Arch. Microbiol. 178: 421-427   DOI
31 박종근. 2005. 대청호의 남조세균 수화 발달 특성, 환경생물 23: 304-314   과학기술학회마을
32 Otsuka, S., S. Suda, R. Li, M. Watanabe, H. Oyaizu, S. Matsumoto and M.M. Watanabe. 1998. 16S rDNA sequence and phylogenetic analysis of Microcystis strains with and without phycoerythrin. FEMS Microbiol. Lett. 164: 119-124   DOI
33 Poon, K.F., M.H. Lam, P.K Lam and B.S. Wong. 2001. Determination of microcystins in cyanobacterial blooms by solid-phase microextraction-high-performance-liquid -chromatography. Environ. Toxicol. Chem. 20: 1648-1655   DOI
34 Sabour, B., M. Loudiki, B. Oudra, V. Vasconcelos, R. Martins, S. Oubraim and B. Fawzi. 2002. Toxicology of a Microcystis ichthyoblabe Waterbloom from Lake Oued Mellah (Morocco). Environ. Toxicol. 17: 24-31   DOI   ScienceOn
35 Baker, J.A., B. Entsch, B.A. Neilan and D.B. McKay. 2002. Monitoring changing toxigenicity of a cyanobacterial bloom by molecular methods. Appl. Environ. Microbiol. 68: 6070-6076   DOI