Browse > Article

Vertical Profiles of Alkaline Phosphatase Activity in Dam Reservoirs and its Relation with Microbial Parameters  

Nakagawa, Ayumi (Faculty of Agriculture, Ehime Univ)
Kagawa, Hisanori (Faculty of Agriculture, Ehime Univ)
Hiroshi, Hirotani (Dept. of Arts and Sciences, Osaka Kyoiku Univ)
Publication Information
Abstract
The alkaline phosphatase activity (APA) of two dam reservoirs and inflowing streams were measured monthly in 2000. During summer months in 2001, the vertical profiles of APA and related parameters were also examined in one of the reservoirs. The APA was relatively high during the summer season in the epilimnion while it was almost invariable in the hypolimnion. A small increase in APA was observed at just above the bottom. The APA fluctuation was independent of the concentration of soluble reactive phosphorus. It was assumed that APA is not indicative of the phosphorus availability status. An examination of size-fractionated samples suggested that APA in reservoirs was attached to particles larger than $0.4{\mu}m$, whereas in streams it existed in a dissolved form. There was a positive significant correlation between chlorophyll a concentration and APA in the photic zone. In the aphotic zone, APA correlated positively with the colony count of heterotrophic bacteria, but not with microscopic total bacterial counts.
Keywords
alkaline phosphatase activity; dam reservoir; photic and aphotic zones; chlorophyll a; heterotrophic bacteria;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Berman, T. 1970. Alkaline phosphatases and phosphorus availability in lake Kinneret. Limnol. Oceanogr. 15: 663-674
2 Hirotani, H., A. Nagagawa, K. Ochi and M. Daijo. 2001. Fluctuation of alkaline phosphatase activity in stream and reservoir water in adjacent watersheds (in Japanese). J. Japan Soc. Water Environ. 24: 762-765
3 Hirotani, H., A. Nagagawa and H. kagawa. 2004. Vertical profile of alkaline phosphatase activity in the water column of a dam reservoir (in Japanese). J. Japan Soc. Water Environ. 27: 175-180
4 Hirotani, H., K. Ochi and A. Nakagawa. 2003. Fluctuation of alkaline phosphatase activity in the headwaters and the factors affecting the activity. IWA-Asia Pacific Regional Conference (Asian Waterqual 2003), Bangkok, October, Proceedings 408 (3Q5K15:1-5)
5 Obst, U. 1985. Test instructions for measuring the microbial metabolic activity in water samples. Fresenius Z. Anal. Chem. 321: 166-168
6 Francko, D.A. 1983. Size-fractionation of alkaline phosphatase activity in lake water by membrane filtration. J. Fresh. Wat. Ecol. 2: 305-309
7 Lorenzen, C.J. 1967. Determination of chlorophyll and pheopigments: spectrophotometric equations. Limnol. Oceanogr. 12: 343-346
8 Koroleff, F. 1983. Determination of phosphorus, in “Methods of Seawater Analysis” (Eds. Grasshoff, K., Ehrhardt, M. and Kremling, K.), 2nd revised ed., pp. 125-139, Verlag Chemie, Weinheim
9 Thingstad, T.F., U.L. Zwifel and F. Rossoulzadegan. 1988. P limitation of heterotrophic bacteria and phytoplankton in the northwest Mediterranean. Limnol. Oceanogr. 43: 88-94
10 Reichardt, W., J. Overbeck and L. Steubing 1967. Free dissolved enzymes in lake waters. Nature 216: 1345-1347
11 Stewart, A.J. and R.G. Wetzel. 1982. Phytoplankton contribution to alkaline phosphatase activity. Arch. Hydrobiol. 93: 265-271
12 AHPA-AWWA-WPCF, 1995. Standard methods for the examination of water and wastewater, 19th ed
13 Jamet, D., C. Amblard and J. Devaux. 2001. Sizefractionated alkaline phosphatase activity in the hypereutrophic Villerest reservoir (Roanne, France). Wat. Environ. Res. 73: 132-141
14 Porter, K.G. and Y.S. Feig 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25: 943-948
15 Chrost, R.J., W. Siuda and G.Z. Halemejko. 1984. Longterm studies on alkaline phosphatase activity (APA) in a lake with fish-aquaculture in relation to lake eutrophication and phosphorus cycle Arch. Hydrobiol. 70: 1-32