[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.14249/eia.2021.30.1.1

Identification of Freshwater Fish Species in Korea Using Environmental DNA Technique - From the Experiment at the Freshwater Fish Ecological Learning Center in Yangpyeong, Gyeonggi Do -

Kim, Gawoo (Dept. of Landscape Architecture, Graduate School of Environmental Studies, Seoul National University)
Song, Youngkeun (Dept. of Landscape Architecture, Graduate School of Environmental Studies, Seoul National University)

Publication Information

Journal of Environmental Impact Assessment / v.30, no.1, 2021 , pp. 1-12 More about this Journal

Abstract

This study focused on verifying the identification of freshwater fish species in Korea using Environmental DNA (eDNA) technique. The research of DNA is increasing in the field of ecology, since this is more sensitive of identify rather than traditional investigation method. Which is difficult to detect species hidden in water and be easily influenced by diverse factors (sites, bad weather, researchers and so on). We applied the pilot test in aquarium (Freshwater Fish Ecological Learning Center in Yangpyeong, Gyeonggi Do), where freshwater fish species are inhabits. We conducted to sampling and analyzing the sixteen water samples (50 species from 7 orders and 13 families) using MiFish primer set. The results showed that 45 species (90%) was investigated by eDNA. It highlight that eDNA with universal primer is possible to detect freshwater fish species of Korean. However, the errors on species identification seems to be caused by the primer that be not suited perfectly and the pollution such as aquarium, sampling collectors.

Keywords

Freshwater ecosystem; Fish community; Fish species survey; Aquatic ecosystem monitoring;

Citations & Related Records

Reference

1	Miya M, Sato Y, Fukunaga T, Sado T, Poulsen JY, Sato K, Minamoto T, Yamamoto S, Yamanaka H, S Araki H, Kondoh M, Iwasaki W. 2015. MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes : detection of more than 230 subtropical marine species. Royal Society Open Science 2(7): p. 3-34.
2	Nelson JS. 1994. Fishes of the world. 3rd edn Wiley, New York, p. 600.
3	Naiman R, Prieur-Richard A, Arthington A, Dudgeon D, Gessner M, Kawabata Z, Knowler D, O'Keefe J, Leveque C, Soto D, Stiassny M, Sullivan C. 2006. Challenges for freshwater biodiversity research : Science plan and implementation strategy. DIVERSITAS, Report No. 5. DIVERSITAS, Paris, pp. 13-15.
4	Rees HC, Maddison BC, Middleditch DJ, Patmore JR, Gough KC. 2014. The detection of aquatic animal species using environmental DNA - a review of eDNA as a survey tool in ecology. Journal of Applied Ecology 51: 1450-1459. DOI
5	Song YK, Kim JH, Won SY, Park C. 2019. Possibility in identifying species composition of fish communities using the environmental DNA metabarcoding technique - with the preliminary results at urban ecological streams. The Korea Society of Environmental Restoration Technology 22(6): 125-138. [Korean Literature]
6	Thomsen PF, Willerslev E. 2015. Environmental DNA - An emerging tool in conservation for monitoring past and present biodiversity, Biological Conservation 183: 4-18. DOI
7	Takahara T, Minamoto T, Yamanaka H, Doi H, Kawabata Z. 2012. Estimation of fish biomass using environmental DNA. PLoS ONE. 7(4): e35868(1)-e35868(9). DOI
8	Takahara T, Minamoto T, Doi H. 2013. Using environmental DNA to estimate the distribution of an invasive fish species in ponds. PloS one. 8(2): e56584. DOI
9	Wheeler Q, Raven P, Wilson E. 2004. Taxonomy: impediment or expedient. Science 303: 285. DOI
10	Yamamoto S, Masuda R, Sato Y, Sado T, Araki H, Kondoh M, Minamoto T, Miya M. 2017. Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea. Scientific Reports 7(40368): 2-11. DOI
11	Alice V, Francois P, Pierre T. 2009. DNA bar oding for ecologists. Trends in Ecology & Evolution 24(2): 110-117. DOI
12	Ficetola GF, Miaud C, Pompanon F, Taberlet P. 2008. Species detection using environmental DNA from water samples. Biology Letter 4(4): 423-425. DOI
13	Ficetola GF, Coissac E, Zundel S, Riaz T, Shehzad W, Bessiere J, Taberlet P, Pompanon F. 2010. An in silico approach for the evaluation of DNA barcodes. BMC Genomics 11(434): 2-8. DOI
14	Fukumoto S, Shimaru A and Minamoto T. 2015. A basin-scale application of environmental DNA assessment for rare endemic species and closely related exotic species in rivers: a case study of giant salamanders in Japan. Journal of Applied Ecology 52(2): 358-365. DOI
15	Hopkins GW, Freckleton RP. 2002. Declines in the numbers of amateur and professional taxonomists: implications for conservation. Animal Conservation 5: 245-249. DOI
16	Kim HM, Kim SY, Park YS, Lee HJ, Kim KT, Kim Y, Kim HJ, Kawk MH, Lim TY, Park C, Song WK. 2020. Review and application of environmental DNA (eDNA) investigation of terrestrial species in urban ecosystem. The Korea Society of Environmental Restoration Technology 23(2): 69-89. [Korean Literature]
17	Magoc T, Salzberg SL. 2011. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27(21): 2957-2963. DOI