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Imaging of Lung Metastasis Tumor Mouse Model using $[^{18}F]FDG$ Small Animal PET and CT  

Kim, June-Youp (Laboratory of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Woo, Sang-Keun (Laboratory of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Lee, Tae-Sup (Laboratory of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Kim, Kyeong-Min (Laboratory of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Kang, Joo-Hyun (Laboratory of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Woo, Kwang-Sun (Laboratory of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Chung, Wee-Sup (Laboratory of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Jung, Jae-Ho (Laboratory of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Cheon, Gi-Jeong (Laboratory of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Choi, Chang-Woon (Laboratory of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Lim, Sang-Moo (Laboratory of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS))
Publication Information
Nuclear Medicine and Molecular Imaging / v.41, no.1, 2007 , pp. 42-48 More about this Journal
Abstract
Purpose: The purpose of this study is to image metastaic lung melanoma model with optimal pre-conditions for animal handling by using $[^{18}F]FDG$ small animal PET and clinical CT. Materials and Methods: The pre-conditions for lung region tumor imaging were 16-22 h fasting and warming temperature at $30^{\circ}C$. Small animal PET image was obtained at 60 min postinjection of 7.4 MBq $[^{18}F]FDG$ and compared pattern of $[^{18}F]FDG$ uptake and glucose standard uptake value (SUVG) of lung region between Ketamine/Xylazine (Ke/Xy) and Isoflurane (Iso) anesthetized group in normal mice. Metastasis tumor mouse model to lung was established by intravenous injection of B16-F10 cells in C57BL/6 mice. In lung metastasis tumor model, $[^{18}F]FDG$ image was obtained and fused with anatomical clinical CT image. Results: Average blood glucose concentration in normal mice were $128.0{\pm}23.87$ and $86.0{\pm}21.65\;mg/dL$ in Ke/Xy group and Iso group, respectively. Ke/Xy group showed 1.5 fold higher blood glucose concentration than Iso group. Lung to Background ratio (L/B) in SUVG image was $8.6{\pm}0.48$ and $12.1{\pm}0.63$ in Ke/Xy group and Iso group, respectively. In tumor detection in lung region, $[^{18}F]FDG$ image of Iso group was better than that of Ke/Xy group, because of high L/B ratio. Metastatic tumor location in $[^{18}F]FDG$ small animal PET image was confirmed by fusion image using clinical CT. Conclusion: Tumor imaging in small animal lung region with $[^{18}F]FDG$ small animal PET should be considered pre-conditions which fasting, warming and an anesthesia during $[^{18}F]FDG$ uptake. Fused imaging with small animal PET and CT image could be useful for the detection of metastatic tumor in lung region.
Keywords
melanoma; lung metastastic tumor; $[^{18}F]FDG$; small animal PET; clinical CT;
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