Browse > Article

Comparison of $Na^+/I^-$ Symporter Expression Rate in Malignant and Benign Thyroid Diseases: Immunohistochemical Study  

Kang, Do-Young (Department of Nuclear Medicine, Dong-A University College of Medicine)
Jeong, Young-Jin (Department of Nuclear Medicine, Dong-A University College of Medicine)
Lee, Kyung-Eun (Medical Research Center for Cancer Molecular Therapy, Dong-A University College of Medicine)
Park, Heon-Soo (Department of Otolaryngology-Head and Neck Surgery, Dong-A University College of Medicine)
Yoo, Young-Hyun (Medical Science Research Center, Dong-A University College of Medicine)
Roh, Mee-Sook (Medical Research Center for Cancer Molecular Therapy, Dong-A University College of Medicine)
Publication Information
Nuclear Medicine and Molecular Imaging / v.40, no.1, 2006 , pp. 9-15 More about this Journal
Abstract
Purpose: Previous studies have not showed consistent results for the level of expression of sodium/iodide symporter (NIS) in thyroid diseases, especially malignant tumor. We undertook this study to evaluate the distribution of NIS expression in malignant thyroid diseases and compare with that in benign thyroid disease. Materials and Methods: Total patients were 119 cases (Men 15, $48{\pm}13$ yrs). Total number of samples were 205 pieces. In malignant thyroid disease, there were 153 samples: 90 in papillary carcinoma, 4 in follicular carcinoma, 2 in medullary carcinoma and 57 in metastatic lymph node. In benign thyroid disease, there were 52 samples: 36 in goiter/cyst, 11 in thyroiditis and 5 in follicular adenoma. Using immunohistochemical methods, we probed 205 samples with monoclonal anti-NIS Ab. Grading of staining was stored as 0 (negative or absent), 1 (weakly positive), 2 (moderately positive) or 3 (strongly positive). Expression rate (ER) of NIS positivity in individual disease entity was expressed as percentage of total number divided by number in 2 plus 3 grade. Results: ERs of malignant thyroid diseases were 63% in papillary carcinoma, 81% in metastatic lymph node, 71% in follicular carcinoma and 100% in medullary carcinoma. ERs of benign thyroid disease were 53% in goiter/cyst, 64% in thyroiditis and 40% in follicular adenoma. ER of malignant thyroid diseases was higher than benign thyroid diseases (71% vs 54%). Grading of NIS expression in papillary carcinoma or goiter/cyst was heterogeneously distributed in considerable cases. Normal tissue also showed heterogeneous distribution of NIS expression, which was not correlated with that of primary lesion. Conclusion: In papillary thyroid carcinoma, distribution of NIS expression was heterogeneous and increased, and not different compared with that of benign thyroid disease.
Keywords
sodium/iodide symporter(NIS); immunohistochemistry; thyroid carcinoma; benign thyroid disease;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Saito T, Endo T, Kawaguchi A, Ikeda M, Katoh R, Kawaoi A, et al. Increased expression of the sodium/iodide symporter in papillary thyroid carcinomas. J Clin Invest 1998;101:1296-300   DOI   ScienceOn
2 Min J-J, Chung J-K, Lee YJ, Jeong JM, Lee DS, Jang JJ, et al. Relationship between expression of the sodium/iodide symporter and $^{131}I$ uptake in recurrent lesions of differentiated thyroid carcinoma. Eur J Nucl Med 2001;28:639-45   DOI   ScienceOn
3 Wapnir IL, Rijn M, Nowels K, Amenta PS, Walton K, Montgomery K, et al. Immunohistochemical profile of the sodium/iodide symporter in thyroid, breast, and other carcinomas using high density tissue microarrays and conventional sections. J Clin Endocrinol Metab 2003;88:1880-8   DOI
4 Levy O, Ginter CS, Vieja AD, Dai G, Riedel C, Carrasco N. N-linked glycosylation of the thyroid $Na^{+}$/$I^{-}$ symporter (NIS). Thyroid 1997;7(Suppl1):S22
5 Kogai T, Curcio F, Hyman S, Cornford EM, Brent GA, Hershman JM. Induction of follicle formation in long-term cultured normal human thyroid cells treated with thyrotropin stimulates iodide uptake but not sodium/iodide symporter messenger RNA and protein expression. J Clin Endocrinol Metab 2000;167:125-35
6 Vadysirisack DD, Shen DH, Jhiang SM. Correlation of Na/$I^{-}$ symporter expression and activity: Implications of Na/$I^{-}$ symporter as an imaging reporter gene. J Nucl Med 2006;47:182-90
7 Riedel C. Levy O, Carrasco N. Post-transcriptional regulation of the sodium/iodide symporter by thyrotropin. J Biol Chem 2001;276:21458-63   DOI   ScienceOn
8 Filetti S, Bidart JM, Arturi F, Caillou B, Russo D, Schlumberger M. Sodium/iodide symporter: a key transport system in thyroid cancer cell metabolism. Eur J Endocrinol 1999;141:443-57   DOI
9 Smanik PA, Ryu Ky, Theil KS, Mazzaferri EL, Jhiang SM. Expression, exon-intron organization, and chromosome mapping of the human sodium iodide symporter. Endocrinology 1997;138: 3555-8   DOI   ScienceOn
10 Vayre L, Sabourin J-C, Caillou B, Ducreux M, Schlumberger M, Bidart J-M. Immunohistochemial analysis of $Na^{+}$/$I^{-}$ symporter distribution in human extra-thyroidal tissues. Eur J Endocrinol 1999;141:382-6   DOI
11 Castro MR, Bergert ER, Goellner JR, Hay ID, Morris JC. Immunohistochemical analysis of sodium iodide symporter expression in metastatic differentiated thyroid cancer: correlation with radioiodine uptake. J Clin Endocrinol Metab 2001;86: 5627-32   DOI
12 Arturi F, Russo D, Schlumberger M, Villard JA, Caillou B, Vigneri P, et al. Iodide Symporter Gene Expression in Human Thyroid Tumors. J Clin Endocrinol Metab 1998:83:2493-6   DOI
13 Lee C-H, Yang A-H, Chang J-Y, Huang C-L, Chi C-W. Immunohistochemical studies of $Na^{+}$/$I^{-}$symporter in human thyroid tissues - A correlation with clinical thyroid scintigraphy. Chin Med J(Taipei) 2001;64:141-6
14 Chung JK. Sodium iodide symporter: Its role in nuclear medicine. J Nucl Med 2002;43:1188-200
15 Kaminsky SM, Levy O, Salvador C, Dai G, Carrasco N. $Na^{+}/I^{-}$ symporter activity is present in membrane vesicles from thyrotropin-deprived non-$I^{-}$-transporting cultured thyroid cells. Proc Natl Acad Sci USA 1994;91:3789-93
16 Dai G, Levy O, Carrasco N. Cloning and characterization of the thyroid iodide transporter. Nature 1996;379:458-60   DOI   ScienceOn
17 Lewis MR. A 'New' reporter in the field of imaging reporter genes: Correlating gene expression and function of the sodium/iodide symporter. J Nucl Med 2006;47:1-3
18 Hedinger C, Williams ED, Sobin LH. Histological typing of thyroid tumors. In: World Health Organization. International histological classification of tumors. 2nd ed. Berlin : Springer- Verlag: 1988
19 Spitzweg C, Joba W, Eisenmenger W, Heufelder AE. Analysis of human sodium iodide symporter gene expression in extrathyroidal tissues and cloning of its complementary deoxyribonucleic acids from salivary gland, mammary gland, and gastric mucosa. J Clin Endocrinol Metab 1998;83:1746-51   DOI
20 Smanik PA, Liu Q, Furminger TL, Ryu K-Y, Xing S, Mazzaferri EL, et al. Cloning of the human sodium iodide symporter. Biochem. Biophys Res Commun 1996;226:339-45   DOI   ScienceOn
21 Caillou B, Troalen F, Baudin E, Talbot M, Filetti S, Schlumberger M, et al. $Na^{+}$/$I^{-}$ symporter distribution in human thyroid tissues: An immunohistochemical study. J Clin Endocrinol Metab 1998;83:4102-6   DOI
22 Carrasco N. Thyroid iodide transport: The $Na^{+}/I^{-}$ symporter (NIS). In: Braverman LE, Utiger RD, editors. The thyroid. 8th ed. Philadelphia : Lippincott Williams & Wilkins; 2000. p. 52-61