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In Vivo Nuclear Imaging of Apoptosis  

Lee, Tae-Sup (Laboratory of Cyclotron Application, Korea Institute of Radiological and Medical Sciences)
Cheon, Gi-Jeong (Department of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences)
Publication Information
The Korean Journal of Nuclear Medicine / v.38, no.2, 2004 , pp. 190-197 More about this Journal
Abstract
Apoptosis plays a role in the pathophysiology of many kinds of diseases and in the response of treatment. Compared to the necrosis, the apoptosis is a genetically controlled and energy-dependent process which removes the unwanted cells from the body; programmed cell death or cell suicide. During the apoptosis, phosphatidylserine is expressed in the cytoplasmic outer membrane in the early phase. Annexin V, an endogenous human protein (MW=35 kD), has an affinity of about $10^{-9}\;M$ for the phosphatidylserine exposed on the outer membrane of apoptotic cells. Annexin V can be radiolabeled with $^{99m}Tc$ by HYNIC or EC chelators, which can be used as an radiotracer for the in vivo imaging of apoptosis. In this article, we reviewed the apoptosis, radiolabeling of annexin V, and the experimental and clinical data using annexin V imaging.
Keywords
Aapoptosis; $^{99m}Tc$-annexin V;
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