Browse > Article
http://dx.doi.org/10.14578/jkfs.2018.107.3.258

Optimization Conditions for Cryopreservation of Potentilla discolor Bunge  

Yang, Woo Hyeong (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
Yong, Seong Hyeon (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
Park, Dongjin (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
Seol, Yuwon (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
Choi, Eunji (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
Jeong, Mi Jin (Plant Conservation Division, Korea National Arboretum of the Korea Forest Service)
Choi, Myung Suk (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
Publication Information
Journal of Korean Society of Forest Science / v.107, no.3, 2018 , pp. 258-265 More about this Journal
Abstract
This study was conducted to investigate the effective cryopreservation condition of Potentilla discolor Bunge, a rare native plant. Seed viability was more than 80% in PVS2 and PVS3 solution treatments. Seed viability was higher in PVS3 than PVS2 treatment. Seed viability was lower than control in spite of sucrose pretreatment. The germination rate was 95% at 60 min of PVS2 treatment and 30 min of PVS3 treatment but the germination rate was low at other treatments. The growth of the seedling wasn't doing that of the control except for treat PVS2 and PVS3 solution for 30 min. There was no statistically significant difference between the encapsulation method and the vitrification method in the seedling growth between the two cryogenic storage methods. This study is expected to be applied to future conservation methods of Potentilla discolor.
Keywords
cyropreservation; encapsulation; fresh weight; germination rate; vitrification;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Niino, T.A., Yakuwa, S.S.H. and Nojiro, K. 1992. Cryopreservation of in vitro grown shoot tips of apple and pear by vitrification. Plant Cell Tissue and Organ Culture. 28: 261-266.   DOI
2 Panis, B. 2008. Cryopreservation of monocots. In: Reed, B. (Ed.), Plant Cryopreservation: A Practical Guide - Springer pp. 241-280. New York.
3 Park, H.J., Lee, K.T. and Park, J.H. 2007. Isolation of two steroids and a triterpenoid from the roots of Potentilla discolor. Korean Journal of Pharmacognosy 38: 354-357.
4 Pawlowska, B. and Szewczyk-Taranek, B. 2014. Droplet vitrification cryopreservation of Rosa canina and Rosa rubiginosa using shoot tips from in situ plants. Scientia Horticulturae 168: 151-156.   DOI
5 Porter, R., Durrell, M. and Romm, H. 1947. The use of 2, 3, 5-triphenyl-tetrazoliumchloride as a measure of seed germinability. Plant Physiology 22: 149-159.   DOI
6 Pritchard, H.W., Poyner, A.L.C. and Seaton, P.T. 1999. Interspecific variation in orchid seed longevity in relation to ultra-dry storage and cryopreservation. Lindleyana 14: 92-101.
7 Rajametov, S., Lee, Y.Y., Kim, Y.C., Lee, S., Yi, J.Y., Jeon, Y.A., Sung, J.S., Lee, G.A. and Gwak, J.G. 2014. Response of pre and post treatments for cryopreservation of Korean ginseng seeds on recovering viability. Korean Journal of Breeding Science 46: 408-416.   DOI
8 Reed, B.M. 2011. Choosing and applying cryopreservation protocols to new plant species or tissues. Acta Horticulturae 908: 363-372.
9 Sakai A., Kobayashi, S. and Oiyama, I. 1990. Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Reports 9: 30-3.
10 Sakai, A. and Engelmann, F. 2007. Vitrification, encapsulation -vitrification and droplet-vitrification: a review. Cryo Letters 28: 151-172.
11 Sakai, A. and Kobayashi, S. 1990. A simple and efficient procedure for cryopreservation of nuclear cells of naverl orange by vitrification. Cryobiology 27: 657.
12 Salomaao, A.N., Santos, I.R.I., Josee, S.C.B.R., Silva, J.P.D. and Laviola, B.G. 2016. Methods to assess the viability of cryopreserved Jatropha curcas L. seed germplasm. Recista Brasileira de Plantas Medicinais 18: 391-398.   DOI
13 Schäfer-Menuhr, A., Schumacher H.M. and Mix-Wagner, G. 1997. Long-term storage of old potato varieties by cryopreservation of shoot tips in liquid nitrogen. Plant Genetic Resources Newsletter 111: 19-24.
14 Seaton, P.T. and Hailes, N.S.J. 1989. Effect of temperature and moisture content on the viability of Cattleya aurantiaca seed. In: Pritchard HW (ed) Modern methods in orchid conservation: the role of physiology, ecology and management. Cambridge University Press. Cambridge, U.K. pp. 17-29.
15 Seijo, G. 2000. Effects of preculture with sucrose and ABA on cell suspensions water status and its relation with vitrification resistance. Brazilian Journal of Plant Physiology 12: 166-180.
16 Stanwood, P.C. 1980. Tolerance of crop seeds to cooling and storage in liquid nitrogen. Journal of Seed Technology 5: 26-31.
17 Stanwood, P.C. and Bass, L.N., 1981. Seed germplasm preservation using liquid nitrogen. Seed Science and Technology 9: 423-437.
18 Benell, C., Carlo, A.D. and Engelmann, F. 2013. Recent advances in the cryopreservation of shoot-derived germplasm of economically important fruit trees of Actinidia, Diospyros, Malus, Olea, Prunus, Pyrus and Vitis. Biotechnology Advances 31: 175-185.   DOI
19 Ahn, Y.H. and Sakai, A. 1994. Survival by vitirification of shoot tips of Chrysanthermum morifolium L. cooled to -195${\circ}C$. Journal of Korean Society for Horticultural Science 35: 451-457.
20 Barrientos-Priego, A.F., Borys, M.W., Escamilla-Prado, E., Ben-Ya'acov, A., Cruz-Torres, E. and Lopez-Lopez, L. 1992. A study of the avocado germplasm resources, 1988 -1990 IV Findings in the Mexican Gulf region. Proceedings of the Second World Avocado Congress. 551-558.
21 Benson, E.E. 2008. Cryopreservation theory. pp.15-32. In: Reed, B.M. (Ed.) Plant Cryopreservation: A Practical Guide. Springer, New York, U.S.A.
22 Choi, Y.C., Ryu, K.S. and Bang, H.S. 2000. Cryopreservation of Mulberry(Morus) Seeds in Liquid Nitrogen(LN2). Korean Journal of Sericultural and Entomological Science 42: 1-5.
23 Engelmann, F. 1991. In vitro conservation of tropical plant germplasm - a review. Euphytica 57: 227-243.   DOI
24 Engelmann, F. 2004. Plant cryopreservation: progress and prospects. In Vitro Cellular & Developmental Biology - Plant 40: 427-433.   DOI
25 Engelmann, F. 2011. Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cellular & Developmental Biology - Plant. 47: 17-25.
26 Engelmann, F. 2012. Germplasm collection, storage and preservation. In: Altman A, Hazegawa PM, editors. Plant biotechnology and agriculture - Prospects for the 21st Century. Oxford: Academic Press. New York, U.S.A. pp. 255-268.
27 Engelmann, F., Dumet, D., Chabrillange, N., Abdelnour-Esquivel, A., Assy-Bah, B., Dereuddre, J. and Duval, Y. 1995. Factors affecting the cryopreservation of coffee, coconut and oil palm embryos. Plant Genetic Resources News 103: 27-31.
28 Fahy, G.M., MacFarlane, D.R., Angell, C.A. and Meryman, H.T. 1984. Vitrification as an approach to cryopreservation. Cryobiology 21: 407-26.   DOI
29 Flachsland, E., Terada, G., Scocchi, A., Mroginski, L. and Engelmann, F. 2006. Cryopreservation of Seed and in vitiro-cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration. Cryo Letters 27(4): 235-42.
30 Ferrari, E.A.P., Columbo, R.C., Faria, R.T., Takane, R.J. 2016. Cryopreservation of seeds of Encholirium spectabile Martius ex Schultes f. by the vitrification method. Revista Ciencia Agronomica 47: 172-177.
31 Galdiano, R.F., Lemos, E.G.M., Faria, R.T. and Vendrame, W.A. 2012. Cryopreservation of Dendrobium hybrid seeds and protocorms as affected by phloroglucinol and Supercool X1000. Journal of Scientia Horticulturae 148: 154-160.   DOI
32 Gonzalez-Arnao, M.T., Panta, A., Roca, W.M., Escobar, R.H. and Engelmann, F. 2008 Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops. Plant Cell Tissue and Organ Culture 92: 1-13.
33 Gonzalez-Arnao, M.T. and Engelmann, F. 2006. Cryopreservation of plant germplasm using the encapsulation-dehydration technique: review and case study on sugarcane. Cryo Letters 27: 155-68.
34 Hu, W.H., Yang, Y.H., Liaw, S.I. and Chang, C. 2013. Cryopreservation the seeds of a Taiwanese terrestrial orchid, Bletilla formosana (Hayata) Schltr. by vitrification. Botanical studies 54: 33.   DOI
35 Jeong, H.R., Choi, K. and Park, K.W. 2012. The regional folk plants in Southern Inland area of Gyeonggi-do. Korean Journal of Plant Resources 25: 523-542.   DOI
36 Jitsopakul, N., Thammasiri, K., Yukawa, T. and Ishikawa, K. 2012. Effect of cryopreservation on seed germination and protocorm development of Vanda tricolor. ScienceAsia 38: 244-249.   DOI
37 Kuranuki, Y. and Sakai, A. 1995. Cryopreservation of in vitro grown shoot tips of tea (Camelia sinensis) by vitrification. Cryo Letters 16: 345-352.
38 Kaviani, B. 2011. Conservation of plant genetic resources by cryopreservation. Australian Journal of Crop Science 5: 778-800.
39 Kim, H.H., Lee, Y.G., Ko, H.C., Gwag, J.G., Cho, E.G. and Engelmann, F. 2009. Development of alternative plant vitrification solutions in droplet-vitrification procedures. Cryo Letters 30: 320-334.
40 Korea National Arboretum. 2009. Rare Plants Data Book of Korea. Geobook Publishing. Seoul, Korea pp.332.
41 Langis, R., Schnable, B., Earle, E.D. and Steponkus, P.L. 1989. Cryopreservation of Brassica campestris L. cell suspensions by vitrification. Cryo Letters 10: 421-8.
42 Ma, Y.Y., Zhang, Y.L,, Lu, J. and Shao, H.B. 2009. Roles of plant soluble sugars and their responses to plant cold stress. African Journal of Biotechnology 8: 2004-2010.
43 Matsumoto, T., Sakai, A. and Yamada, K. 1994. Cryopreservation of in vitro grown apical meristems of Wasabi (Wasabia japonica) by vitrification and subsequent high plant regeneration. Plant Cell Reports 13: 442-446.
44 Michalak, M., Plitta, B.P. and Chmielarz, P. 2013. Desiccation sensitivity and successful cryopreservation of oil seeds of European hazelnut (Corylus avellana). Annals of Applied Biology 163: 351-358.
45 Murashige, T. and Skoog, F. 1962. A revise medium for rapid growth and bioassay with tobacco tissue culture. Physiologia Plantarum 15: 473-497.   DOI