[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.14578/jkfs.2014.103.1.72

Effect of Culture Medium Strength, Plant Growth Regulators and Ethylene Inhibitors for Adventitious Bud Induction from Mature Zygotic Embryo in Larix kaempferi

Kim, Yong Wook (Division of Tree Biotechnology, Korea Forestry Research Institute)

Publication Information

Journal of Korean Society of Forest Science / v.103, no.1, 2014 , pp. 72-79 More about this Journal

Abstract

Adventitious buds were produced from the cultures of mature zygotic embryos of Larix kaempferi with the highest frequency in Quoirin & Lepoivre (LP) medium containing 1.0 mg/L zeatin (76.1%). The effective treatments for inducing adventitious shoots growth above 2 mm were shown in Litvay (LM) medium with 0.5 mg/L zeatin (75.2%) or LP medium with 2.0 mg/L zeatin (70.2%), respectively. In experiment with half strength salts medium for induction of the adventitious buds, the effective treatments were obtained from 1/2LP medium with 1.0 (83.3%) or 2.0 mg/L (81.7%) zeatin, respectively. However, the best adventitious shoot growth more than 2 mm appeared in 1/2LM medium with 1.0 mg/L zeatin (66.7%). In experiment with half strength salts medium for induction of the adventitious buds, the effective treatments were obtained from 1/2LP medium with 1.0 (83.3%) or 2.0 mg/L (81.7%) zeatin, respectively. However, the best adventitious shoot growth more than 2 mm appeared in 1/2LM medium with 1.0 mg/L zeatin (66.7%). In experiment of subsequent treatment with various cytokinins for induction of the adventitious buds, the best one (52.9%) was obtained from 1.0 mg/L zeatin for 2weeks, and then subcultured to the medium with 1.0 mg/L thidiazuron (TDZ). The effect of ethylene synergist or inhibitor on adventitious buds induction was examined. The highest rate (34.6%) of adventitious buds marked from the treatments of 1.0 mg/L zeatin+2.0 mg/L MGBG (methylglyoxal bis-[guanylhydrazone]). And the highest no. of adventitious buds(1.5/explant) was shown in the medium with 1.0 mg/L zeatin+2.0 mg/L $CoCl_2$ .

Keywords

Japanese larch; mature zygotic embryo; rooting; shoot growth;

Citations & Related Records

Reference

1	Chesick, E.E., Bilderback, D.E., and Blake, G.M. 1990. In vitro multiple bud formation by 20-year-old western larch buds and stems. HortScience 25(1): 114-116.
2	Afele, J.C., and Preveen, K.S. 1995. Somatic embryogenesis in blue spruce (Picea pungens Engelmann). In: Somatic Embryogenesis in Woody Plants, Jain S, Gupta P and Newton RE (eds.). Kluwer Academic Publisher, Dordrecht, Boston and London, pp. 99-109.
3	Bercetche, J. and Paques, M. 1995. Somatic embryogenesis in maritime pine (Pinus pinaster). In: Somatic embryogenesis in woody plants, Jain S, Gupta P and Newton RE (eds). Kluwer Academic Publisher, Dordrecht, Boston and London, pp. 221-242.
4	Biddington, N.L. 1992. The influence of ethylene in plant tissue culture. Plant Growth Regulation 11(2): 173-187. DOI ScienceOn
5	Bonga, J.M. and McInnis, A.H. 1983. Origin and early development of roots in plantlets derived from embryo sections of Larix laricina in vitro. Canadian Forestry Service Research 3(1): 12-14.
6	Bonga, J.M. and Pond, S.E. 1991. Adventitious shoot formation in cultures of 30-year-old Larix decidua, L. leptolepis, L. eurolepis, and L. laricina trees. Plant Cell, Tissue and Organ Culture 26(1): 45-51. DOI
7	Diner, A.M., Strickler, A., and Karnosky, D. 1986. Initiation, elongation and remultiplication of Larix decidua micropropagules. New Zealand Journal of Forestry Science 16(2): 306-318.
8	Ellis, D.D., Barczynska, H., McCown, B.H., and Nelson, N. 1991. A comparison of BAP, zeatin and thidiazuron for adventitious bud formation from Picea glauca embryos and epicotyl explants. Plant Cell, Tissue and Organ Culture 27(3): 281-287. DOI
9	Flygh, G., Gronroos, R., and von Arnold, S. 1993. Induction, rooting, and growth capacity of adventitious shoots of Pinus contorta. Canadian Journal Forest Research 23(9): 1907-1916. DOI ScienceOn
10	Harry, I.S., Pulido, C.M., and Thorpe, T.A. 1995. Plantlet regeneration from mature embryos of Juniperus cedrus. Plant Cell, Tissue and Organ Culture 41(1): 75-78. DOI
11	Harry, I.S., Thompson, M.R., Lu, C.Y., and Thorpe, T.A. 1987. In vitro plantlet formation from embryonic explants of eastern white cedar (Thuja occidentalis L). Tree Physiology 3(3): 273-283. DOI ScienceOn
12	Hatanaka, T., Sawabe, E., Azuma, T., and Uchida, N. 1995. The role of ethylene in somatic embryogenesis from leaf discs of Coffea canephora. Plant Science 107(2): 199-204. DOI ScienceOn
13	Lambardi, M., Sharma, K.K., and Thorpe, T.A. 1991. In vitro regeneration of shoot buds and plantlet formation from mature embryos of Pinus halepensis Mill. Acta Horticulturae 289(3): 123-124.
14	Karnosky, D.F. and Diner, A.M. 1984. Techniques for increasing clone sizes in Larix. In: Proceeding 6th International Congress Plant Tissue Cell Culture University Minnesota, Minneapolis, p. 10.
15	Litvay, J.D., Verma, D.C., and Johnson, M.A. 1985. Influence of a loblolly pine (Pinus taeda L.) culture medim and its components on growth and somatic embryogenesis of the wild carrot (Darcus carota L.). Plant Cell Reports 4(6): 325-328. DOI ScienceOn
16	Kumar, P.P., Richard, W.I., and Thorpe, T.A. 1989. Ethylene and carbon dioxide accumulation, and growth of cell suspension cultures of Picea glauca (white spruce). Journal of Plant Physiology 135(5): 592-596.
17	Lambardi, M., Sharma, K.K. and Thorpe, T.A. 1993. Optimization of in vitro bud induction and plantlet formation from mature embryos of aleppo pine (Pinus halepensis Mill.). In Vitro Cellular and Developmental Biology-Plant 29(4): 189-199.
18	Lapp, M.S., Malinek, J., and Coffey, M. 1995. Micropropagation of western white pine (Pinus monticola) starting with mature embryos. Plant Cell Reports 15(3-4): 264-267.
19	Morgenstern, E.K. 1987. Methods for rooting of larch cuttings and application in clonal selection. Forest Chronicle 63(3): 174-178. DOI
20	Muriithi, W.T., Harry, L.S., Yeung, E.C., and Thorpe, T.A. 1993. Plantlet regeneration in chir pine (Pinus roxburghii Sarg.): morphogenesis and histology. Forest Ecology Management 57(1-4): 141-160. DOI ScienceOn
21	Pulido, C.M., Harry, I.S., and Thorpe, T.A. 1994. Effect of various bud induction treatments on elongation and rooting of adventitious shoots of Canary Island pine (Pinus canariensis). Plant Cell, Tissue and Organ Culture 39(3): 225-230. DOI ScienceOn
22	Palmer, C.E. 1992. Enhanced shoot regeneration from Brassica campestris by silver nitrate. Plant Cell Reports 11(11): 541-545.
23	Roustan, J.P., Latche, A., and Fallot, J. 1990. Control of carrot somatic embryogenesis by AgNO3, an inhibitor of ethylene action: effect on arginine decarboxylase activity. Plant Science 67(1): 89-95. DOI ScienceOn
24	Patel, K.R. and Thorpe, T.A. 1984. In vitro differentiation of plantlets from embryonic explants of lodgepole pine (Pinus contorta Dougl. ex Loud.). Plant Cell, Tissue and Organ Culture 3(2): 131-142. DOI
25	Patel, K.R., Kim, H.R., and Thorpe, T.A. 1986. Plantlet formation in pitch pine (Pinus rigida Mill.) by tissue culture methods. Forest Ecology Management 15(2): 147-160. DOI ScienceOn
26	Quoirin, M. and Lepoivre, P. 1977. E'tude de milleux adates aux cultures in vitro de Prunus. Acta Horticulturae 78: 439-432.
27	von Arnold, S. and Eriksson, T. 1981. In vitro studies of adventitious shoot formation in Pinus contorta. Canadian Journal of Botany 59(5): 870-874. DOI
28	Zel, J., Gogala, N., and Camloh, M. 1988. Micropropagation of Pinus sylvestris. Plant Cell, Tissue and Organ Culture 14(3): 169-175. DOI
29	Kong, L.S., and Yeung, E.C. 1994. Effects of ethylene and ethylene inhibitors on white spruce somatic embryo maturation. Plant Science 104(1): 71-80. DOI ScienceOn