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http://dx.doi.org/10.14578/jkfs.2013.102.1.129

Stem Girding Increases Seed Production and Nitrogenous Compounds in Larix leptolepis  

Lee, Wi Young (Division of Forest Tree Improvement, Korea Forest Research Institute)
Park, Eung-Jun (Division of Forest Biotechnology, Korea Forest Research Institute)
Publication Information
Journal of Korean Society of Forest Science / v.102, no.1, 2013 , pp. 129-135 More about this Journal
Abstract
Japanese larch (Larix leptolepis) has been extensively planted in Korea as a reforestation species but their supply has been a major bottleneck due to sporadic natural seed production. In this study, stem girdling was applied to 32-year-old grafted Japanese larches, resulting in significantly enhanced seed production compared to the controls. Stem girdling induced about 4 times higher cone production than that of controls in the Japanese larch seed orchard. Time-dependent metabolic alterations after girdling were investigated by stable isotope ratio mass spectrometer, HPLC, and GC-MS analysis. In girdled trees, the contents of total nitrogen, sucrose, and total free amino acids were significantly higher than the non-girdled trees at the flowering differentiation season (from July to August). Moreover, the numbers of female strobilus per tree were positively correlated with the contents of both total nitrogen (r=0.765, p<0.01) and total amino acids (r=0.802, p<0.01) in the bark being collected at the flowering differentiation time (August 20). Interestingly, the levels of various individual amino acids at the flowering differentiation times, such as aspartic acid, glutamic acid, glycine, serine, and cysteine, were also significantly correlated (p<0.05) with the numbers of strobilus, suggesting that those amino acids might be involved in the induction of female strobilus formation of Japanese larches.
Keywords
Larix leptolepis; seed production; metabolite; girdling; nitrogenous compounds;
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