Browse > Article

Effect of Soil Environment on Diversity and Population of Aerobic Soil Bacteria from Baekdudaegan Mountain Forests in Gyeongsangbuk-do, Korea  

Park, Chul Yeong (Department of Forest Environment Protection, Kangwon National University)
Lee, Sun Keun (Department of Forest Environment Protection, Kangwon National University)
Kim, Ji Hong (Department of Forest Management, Kangwon National University)
Lee, Sang Yong (Department of Forest Environment Protection, Kangwon National University)
Lee, Jong Kyu (Department of Forest Environment Protection, Kangwon National University)
Publication Information
Journal of Korean Society of Forest Science / v.101, no.3, 2012 , pp. 501-508 More about this Journal
Abstract
This study was carried out to compare species diversity of soil bacteria from Baekdudaegan mountain forests (Bonghwa-gun, Mungyeong-si and Sangju-si) in Gyeongsangbuk-do and to analyze the effects of soil environments on diversity and population of soil bacteria. Soil bacteria were isolated from soil samples by streak plate method, and identified by DNA extaction and 16S rDNA sequence analyses. The population of soil bacteria from the soil samples of Bonghwa-gun was the highest with $5.1{\times}10^5cfu/g$, and followed by those from Mungyeong-si and Sangju-si with $1.9{\times}10^5cfu/g$ and $1.1{\times}10^5cfu/g$, respectively. The population of soil bacteria from surface layer soil was the highest, and then gradually decreased according to soil depth. The increase in population of soil bacteria from soil samples of different sites was correlated with the increase of the altitude of soil sampling site, depth of A horizon, liquid phase among three phases of soil, water content and bulk density of soil. Two hundreds and sixty eight bacterial colonies from Bonghwa-gun were classified into 10 species, 8 genera. One hundred and thirty four bacterial colonies from Mungyeong-si were classified into 15 species, 9 genera. Forty four bacterial colonies from Sangju-si were classified into 5 species, 2 genera. The dominant species (occupancy rate) from Bonghwa-gun and Mungyeong-si were Bacillus weihenstephanensis (36% and 40%, respectively), and Sangju-si was Bacillus cereus (39%). The relationships between soil environment and community structure of soil bacteria were analyzed statistically by using ecological indices. The diversity, evenness and dominance indices of soil bacteria were 6.30, 2.04 and 0.59 in Bonghwa-gun, 9.09, 2.94 and 0.51 in Mungyeong-si, and 4.55, 2.34 and 0.71 in Sangju-si, respectively. The diversity and evenness indices were increased by the increase of water content, drainage condition and gravel content of soil, while the dominance index was decreased.
Keywords
soil bacteria; soil environment; population; diversity; ecological indices; Bacillus;
Citations & Related Records
연도 인용수 순위
  • Reference
1 강원대. 2011. 백두대간 산림생물다양성 사업단 1차년도 연차 실적.계획서. pp. 108-135.
2 경상북도. 2010. 경상북도 2010년 통계연보.
3 김남규. 2006. 오대산 국립공원내 임상별 토양미생물 및 고등 균류상에 대한 연구. 농학석사학위논문. 강원대학교.
4 김성욱, 황인규, 박병근, 황의일, 김영태, 이재준, 이향복, 김나래. 2000. 항진균성 생물농약 소재를 개발하기 위한 길항미생물 Bacillus의 육종. 과학기술부.
5 김태훈, 정진현, 구교상, 김규훈, 차순형, 김준섭, 이충화, 구창덕. 1988. 산림토양분석에 관한 연구. 임업연구원 연구보고 37: 19-34.
6 문경시. 2010. 문경시 연보.
7 봉화군. 2010. 봉화군 2010년 통계연보.
8 산림청. 2008. 백두대간 2008년도 현지조사정보.
9 상주시. 2010. 상주시 2010년 통계연보(토지기후).
10 성치남, 백근식, 김종홍. 1998. 백두산의 식생에 따른 토양 미생물의 분포 및 특성. 한국생태학회지 21(5-2): 575-583.   과학기술학회마을
11 손희성, 한송이, 황경숙. 2007. DNA 직접추출법에 따른 산림토양 부식층 내 세균군집의 계통학적 다양성 비교. 미생물학회지 43(3): 210-216.   과학기술학회마을
12 양창술 외. 2002. 토양미생물실험법. 월드사이언스. 서울. pp.453.
13 한송이, 김윤지, 황경숙. 2006. 16S rDNA-ARDRA법을 이용한 소나무림과 상수리나무림 토양 내 VBNC 세균군집의 계통학적 특성 비교. 미생물학회지 42(2): 116-124.   과학기술학회마을
14 Anderson, J.P.E. and Domsch, K.H. 1978. A physiological method for the quantitative measurement for microbial biomass in soil. Soil Biology & Biochemistry 10: 210-221.
15 Bavykin, S.G., Lysov, Y.P., Zakhariev, V., Kelly, J.J., Jackman, J., Stahl, D.A. and Cherni, A. 2004. Use of 16S rRNA, 23S rRNA, and gyrB Gene sequence analysis to determine phylogenetic relationships of Bacillus cereus group microorganisms. Journal of Clinical Microbiology 42(8): 3711-3730.   DOI   ScienceOn
16 Dommergues, Y.R., Belser, L.W. and Schmidt, E.L. 1978. Limiting factors for microbial growth and activity in soil. In Alexander, M. (ed.), Advences in Microbial Ecology. Plenum Press, N.Y.
17 Ekelund, F., Ronn, R. and Christensen, S. 2001. Distribution with depth of protozoa, bacteria and fungi in soil profiles from three Danish forest sites. Soil Biology & Biochemistry 33: 475-481.   DOI   ScienceOn
18 Goris, J., Vos, P.D., Caballero-mellado, J., Park, J.H., Falsen, E., Quensen, III J.F., Tiedje, J.M. and Vandamme, P. 2004. Classification of the biphenyl- and polychlorinated biphenyl-degrading strain LB400T and relatives as Burkholderia xenovorans sp. nov.. International Journal of Systematic and Evolutionary Microbiology 54: 1677-1681.   DOI   ScienceOn
19 Groot, M.N., Nieboer, F. and Abee, T. 2008. Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA. Applied and Environmental Microbiology 74(24): 7817-7820.   DOI   ScienceOn
20 Hill, T.C.J., Walsh, K.A., Harris, J.A. and Moffett, B.F. 2003. Using ecological diversity measures with bacterial communities. Microbiology Ecology 43: 1-11.   DOI   ScienceOn
21 Mandelbaum, R.T., Allam, D.L. and Wackett, L.P. 1995. Isolation and characterization of a Pseudomonas sp. that mineralizes the s-Triazine Herbicide Atrazine. Applied and Environmental Microbiology 61(4): 1451-1457.
22 Smit, E., Leeflang, P., Gommans, S., Broek, J.V.D., Mil, S.V. and Wernars, K. 2001. Diversity and seasonal fluctuations of the dominant members of the bacterial soil community in a wheat field as determined by cultivation and molecular methods. Applied and Environmental Microbiology 67(5): 2284-2291.   DOI   ScienceOn
23 Stephan, A., Meyer, A.H. and Schmid, B. 2000. Plant diversity affects culturable soil bacteria in experimental grassland communities. Journal of Ecology 88: 988-998.   DOI   ScienceOn
24 Tuazon, C.U., Murray, H.W., Levi, C., Solny, M.N., Curtin, J.A. and Sheagren, J.N. 1979. Serious infection from Bacillus sp.. The Journal of the American Medical Association 241(11): 1137-1140.   DOI   ScienceOn
25 Troussellier, M. and Legendre, P. 1981. A functional evenness index for microbial ecology. Microbial Ecology 7: 283-296.   DOI   ScienceOn