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Morphological and Physiological Characteristics of Acclimated Liriodendron tulipifera Tree Produced by Several Types of In Vitro Germination Culture  

An, Chan Hoon (Department of Forest Resources, Graduate School, Kangwon National University)
Yi, Jae Seon (College of Forest and Environmental Sciences, Kangwon National University)
Moon, Heung Kyu (Division of Forest Biotechnology, Korea Forest Research Institute)
Kim, Yong Wook (Division of Forest Biotechnology, Korea Forest Research Institute)
Publication Information
Journal of Korean Society of Forest Science / v.101, no.1, 2012 , pp. 158-162 More about this Journal
Abstract
The acclimatization of in vitro propagated plants is an important step to produce vigorous plants for clonal forestry and in vitro micro-environment may affect the growth in ex vitro condition. To monitor in vitro environmental effects on the growth in ex vitro condition, several culture systems such semi-solid medium(SS), temporary immersion bioreactor(TIB) and continuous immersion bioreactor(CIB) culture types were tested to compare for the growth of acclimated plants of Liriodendron tulipifera. Results suggested that morphological characters, stomatal conductance, evapotranspiration and chlorophyll contents of acclimated plants were affected by the different of in vitro culture conditions. CIB type of culture was resulted to the lowest value in the biomass of acclimated plants. Net photosynthsis rate of CIB was the same level as those of SS and TIB. However, stomatal conductance, evapotranspiration and $CO_2$ partial pressure in the intercellular air space were lower than those of SS and TIB. The amounts of chlorophyll a, b and carotenoids were also lower than those of the other two culture systems. TIB, showing a little lower or higher value than SS in many growth character, is recommended rather than CIB to produce healthy yellow poplar plants in ex situ condition.
Keywords
temporary immersion bioreactor(TIB); continuous immersion bioreactor(CIB); Liriodendron tulipifera; growth trait; acclimatization;
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Times Cited By KSCI : 2  (Citation Analysis)
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