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Plant Regeneration via Secondary Somatic Embryogenesis and Acclimatization in Panax ginseng  

Lee, Su-Gwang (Lab. of Environmental Biotechnology, Department of Environmental & Ecological Engineering, College of Life Science, Dongguk University)
Kim, Ji-Hee (Lab. of Environmental Biotechnology, Department of Environmental & Ecological Engineering, College of Life Science, Dongguk University)
Kang, Ho-Duck (Lab. of Environmental Biotechnology, Department of Environmental & Ecological Engineering, College of Life Science, Dongguk University)
Publication Information
Journal of Korean Society of Forest Science / v.97, no.1, 2008 , pp. 127-133 More about this Journal
Abstract
This study was conducted to establish the optimal condition for plant regeneration and acclimatization from somatic embryos of Panax ginseng. Cotyledon segments of Panax ginseng produced primary and secondary somatic embryos when cultured on MS and WPM media supplemented with 7% sucrose. To induce plantlet conversion, cotyledonary somatic embryos were cultured on WPM solid medium with $GA_3$ at various concentrations (1~30 mg/L) for 4 weeks. Plantlets were transferred to 1/2 WPM solid medium with $GA_3$ at various concentrations (0~5 mg/L) and 0.5% activated charcoal for shoot and root elongations. Elongated plantlets further developed into well-developed leaf and root system on 1/3 SH medium with 0.5% activated charcoal under ventilation condition for 5 months. The highest survival rate to soil was 75% when plantlets were regenerated on 1/3 SH medium without sucrose under ventilation condition.
Keywords
acclimatization; Panax ginseng; somatic embryogenesis; ventilation;
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Times Cited By KSCI : 4  (Citation Analysis)
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