Extractives from the Bark of Platycarya strobilacea

The dried bark of Platycarya strobilacea were ground, extracted with 95% EtOH, concentrated, and one of EtOH extracts was fractionated with a series of n-hexane, dichloromethane and another was fractionated with a series of petroleumether, $Et_2O$, ethyl acetate on a separatory funnel. A portion of dichloromethane soluble was chromatographed on a Sephadex LH-20 column ($72.0{\times}5.0cm$) using EtOH-$CHCl_3$ (7:3, v/v) as eluent and A portion of $Et_2O$ soluble was chromatographed on a silica gel column ($42.0{\times}3.5cm$) using $CHCl_3$-MeOH (9:3, v/v) as eluent. The isolated compounds were identified by TLC, $^1H$-, $^{13}C$-NMR, HMBC and EI-MS. Two flavonoids and three flavonoid glycosides were isolated from the bark of P strobilacea. The structures were determined to quercetin (compound 1), myricetin (compound 2) as flavonol compounds and afzelin (compound 3), quercitrin (compound 4), myricitrin (compound 5) as flavonol glycosides, respectively, on the basis of spectrosopic data.

굴피나무(Platycarya strobilancea) 수피의 Flavonol glycosides

굴피나무 수피를 채취하여 건조시킨 후 분말로 제조하여 95% EtOH로 추출하고 여기서 얻어진 EtOH 조추출물의 하나는 헥산(n-hexane), 디클로로메탄($CH_2Cl_2$)으로, 또 하나는 석유에테르(petroleumether), 디에틸에테르($Et_2O$) 및 에틸아세테이트(EtOAc)를 사용하여 순차적으로 분획하였다. 이중 디클로로메탄($CH_2Cl_2$) 가용부(6.0 g)는 EtOH-$CHCl_3$ (7:3, v/v)를 용출용매로 한 Sephadex LH-20 칼럼($72.0{\times}5.0cm$)을 사용한 칼럼크로마토그래피를 실시하였다. 또한 디에틸에테르($Et_2O$) 가용부는 $CHCl_3$-MeOH (9:3, v/v)을 용출용매로 한 silica gel 칼럼($42.0{\times}3.5cm$)을 사용하여 칼럼크로마토그래피를 실시하였다. 단리된 화합물들은 TLC로 확인한 후 $^1H$-, $^{13}C$-NMR, HMBC 등의 스펙트럼을 사용하여 화학구조를 규명하였고 EI-MS로써 분자량을 측정하였다. 추출물로부터 3, 3', 4', 5, 7-pentahydroxyflavone (quercetin), 3, 3', 4', 5, 5', 7-hexahydroxyflavone (myricetin), flavonoids 배당체인 kaempferol-3-O-${\alpha}$-L-rhamnopyranoside (afzelin), quercetin-3-O-${\alpha}$-L-rhamnopyranoside (quercitrin), myricetin-3-O-${\alpha}$-L-rhamnopyranoside (myricitrin)등을 단리할 수 있었다.