Plant Regeneration from Leaf derived Callus of Hybrid Kiwi (Actinidia deliciosa × A. arguta)

Whole plants were regenerated from callus induced from leaf explants in hybrid kiwi (Actinidia deliciosa${\times}$A. arguta). Callus was induced from leaf explants which cultured on MS solid medium supplemented with combination of auxin (2,4-D, NAA: 0.1~0.5 mg/l) and cytokinin (BA: 0.1~0.2 mg/l). them, the highest callus formation (96.2%) was obtained from the treatment of 0.5 mg/1 2,4-D+0.1 mg/l NAA+0.05 mg/l BA. In the experiment of adventitious shoots induction from primary shoots, only a few shoots were produced in the treatment of 1.0 mg/l BA+0.05 mg/l IBA or 2.0 mg/l BA+0.05 mg/l lBA. As the callus were transferred to the secondary shoot-inducing medium, multiple shoots were obtained from the medium supplemented with 1.0, 2.0 or 5.0 mg/l zeatin in addition to the mixed treatments of BA, thidiazuron (TDZ) or zeatin. However, no multiple shoots were induced on the BA-contained medium of concentrations. Therefore it turned out that addition of BA to medium was less effective for induction of multiple shoots from callus in Actinidia deliciosa${\times}$A. arguta. For producing adventitious roots from shoots, the best frequency of rooting (83.3%) were recorded on the treatment of in vitro rooting (Standardi (St)+1.0 mg/l IBA). On the other side, the lowest result (40.0%) were shown in the treatment of 500 mg/l IBA, 1 hr. Whole plants with shoots and roots were recovered and acclimatized successfully.

잡종키위 (양다래×다래)의 엽조직 캘러스로부터 식물체 재분화

잡종키위(Actinidia deliciosa${\times}$A. arguta) 엽조직의 캘러스로부터 완전한 식물체를 재분화시킬 수 있었다. 캘러스는 옥신류(2,4-D, NAA: 0.1~0.5 mg/l)와 싸이토키닌류(BA: 0.1~0.2 mg/l)를 조함하여 첨가된 MS배지에 엽절편체를 배양하여 캘러스를 유도하였다. 가장 높은 캘러스 유도율(96.2%)은 0.5 mg/1 2,4-D+0.1 mg/l NAA+0.05 mg/l BA의 처리구에서 나타났다. 신초유도는 1차 신초 유도배지에서 1.0 mg/l BA+0.05 mg/l IBA 혹은 2.0 mg/l BA+0.05 mg/l lBA 첨가구에서만 소수의 신초가 유도되었다. 그러나 캘러스를 1.0, 2.0 혹은 5.0 mg/l zeatin의 단독처리구 혹은 BA, TDZ 혹은 zeatin이 첨가된 2차 신초 유도배지처리구에서 다경유도가 가능하였지만 BA 첨가구에는 그 농도에 관계없이 다경유도는 이루어지지 않아 잡종키위의 캘러스로부터 신초유도시 BA첨가는 별 효과가 없는 것으로 나타났다. 신초로부터 발근은 기내발근 [Standardi (St)+1.0 mg/l IBA]시 가장 높은 발근율(83.3%)를 보였지만 500 mg/l IBA의 수용액에 신초를 1시간동안 침지한 처리구에서 가장 저조한 발근율(40.0%)을 보였다. 줄기와 뿌리가 유도된 완전한 식물체를 재분화가 가능하였고 순화 또한 성공적으로 이루어졌다.