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Detection method of Genetic Variation of Mulberry Dwarf Phytoplasma by PCR-SSCP Analysis  

Han, Sangseop (Division of Forest Science, Chonbuk National University)
Cha, Byeongjin (Dept. of Plant medicine, Chonbuk National University)
Seong, Gyoobyoung (National Institute of Agricultural Science and Technology, RDA)
Publication Information
Journal of Korean Society of Forest Science / v.95, no.6, 2006 , pp. 631-635 More about this Journal
Abstract
Single-strand conformation polymorphism (SSCP) analysis of MD and JWB phytopalsma isolates which amplified PCR products using the R16F2n/R2 phytoplamsa universal primer pair were compared for variations of their nucleotide sequence. The MD and JWB phytoplasmas were clearly distinct each of the band patterns from about 1.2 kb PCR products. To clearly distinct of close SSCP band patterns, the MD and JWB phytoplasma PCR products were mixed and performed to detect their polymorphism. The SSCP band patterns show all of bands of MD and JWB on single lane and easily distinct their each band patterns. The PCR-SSCP analysis was possible to detect of 1.2 kb nucleotide sequence and near close band patterns were easily distinct by mixing two samples.
Keywords
SSCP; MD; JWB; phytopalsma; polymorphism;
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Times Cited By KSCI : 2  (Citation Analysis)
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