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Extraction and Determination of Chlorophyll Contents of Korean Pine Needles Using Acetone and DMSO (Dimethylsulfoxide)  

Seo, Keum Young (Division of Environmental Science and Ecological Engineering, Korea University)
Son, Yowhan (Division of Environmental Science and Ecological Engineering, Korea University)
Koo, Jin Woo (Division of Environmental Science and Ecological Engineering, Korea University)
Noh, Nam Jin (Division of Environmental Science and Ecological Engineering, Korea University)
Yi, Myong Jong (College of Forest Sciences, Kangwon National University)
Publication Information
Journal of Korean Society of Forest Science / v.94, no.4, 2005 , pp. 264-268 More about this Journal
Abstract
Chlorophyll contents in Korean pine (Pinus koraiensis) needles of an advance growth stand and a plantation were compared using 80% acetone and DMSO (dimethylsulfoxide). Total chlorophyll contents in DMSO were 4-6 times higher than those in 80% acetone. There were no significant differences in chlorophyll contents between two stand types in both 80% acetone and DMSO. It appeared that 6 hours in DMSO would be appropriate to successfully extract chlorophyll from Korean pine needles of advance growth and planted trees.
Keywords
80% acetone; chlorophyll contents; DMSO; Pinus koraiensis;
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Times Cited By KSCI : 3  (Citation Analysis)
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